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Method for carrying out surface-enhancement Raman spectrum detection on surface of cellular material

A technology of surface-enhanced Raman and porous materials, applied in the field of chemical analysis, can solve the problems of low detection sensitivity and low stability of the enhanced substrate, and achieve a wide range of sources, improve the detection sensitivity, and satisfy the detection effect.

Inactive Publication Date: 2012-12-26
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide an improved method for surface-enhanced Raman spectroscopy detection, which enhances the stability of the substrate. Well, the detection sensitivity is greatly improved, and the detectable concentration is as low as 10 -18 mol / L analyte

Method used

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  • Method for carrying out surface-enhancement Raman spectrum detection on surface of cellular material
  • Method for carrying out surface-enhancement Raman spectrum detection on surface of cellular material
  • Method for carrying out surface-enhancement Raman spectrum detection on surface of cellular material

Examples

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Embodiment 1

[0034] The monolithic column commonly used in chromatographic analysis is a kind of organic porous material with a certain surface morphology and pore size. The following polymethacrylate monolithic column, combined with nano-silver sol, is used for low-concentration rhodamine 6G (R6G) The surface-enhanced Raman detection is taken as an example, and the present invention will be further described in conjunction with the accompanying drawings. See the operation process figure 1 , see the experimental results figure 2 .

[0035] (1) Polymethacrylate monolithic column

[0036] Synthesis of polymethacrylate monolithic column: Weigh (monomer) glycidyl methacrylate GMA 1.2960g, (crosslinking agent) ethylene glycol dimethacrylate EDMA 0.8640g, (initiator) benzyl peroxide Acyl BPO 0.0216g, (porogen) dodecanol 0.5184g and cyclohexanol 2.7216g are poured into a straight plastic mold with a length of 8cm and a diameter of 1.5cm, and blow nitrogen until the BPO is completely dissolved...

Embodiment 2

[0047] The monolithic column commonly used in chromatographic analysis is a kind of organic porous material with a certain surface morphology and pore size. The silica gel monolithic column, combined with nano-gold sol, is used for surface-enhanced Raman of low-concentration rhodamine 6G (R6G). The detection is taken as an example, and the present invention will be further described in conjunction with the accompanying drawings. See the operation process figure 1 , see the experimental results image 3 .

[0048] (1) Silica gel monolithic column

[0049] Polyethylene glycol (PEG) and tetramethoxysilane (TMOS) are mixed in a certain ratio and dissolved in acetic acid solution. Stir for 40 min under ice bath to make it evenly mixed. After the mixture was ultrasonically degassed, it was injected into a straight plastic mold with a length of 10 cm and a diameter of 1.0 cm. At 40°C, let stand for 2 hours to gel. Aged for 24 hours at the same temperature. Then heat treated wi...

Embodiment 3

[0060] The monolithic column commonly used in chromatographic analysis is a kind of organic porous material with a certain surface morphology and pore size. The following polymethacrylate monolithic column, combined with nano-silver sol, is used for surface-enhanced Raman of low-concentration thymine The detection is taken as an example, and the present invention will be further described in conjunction with the accompanying drawings. See the operation process figure 1 , see the experimental results Figure 4 .

[0061] (1) Polymethacrylate monolithic column

[0062] Synthesis of polymethacrylate monolithic column: Weigh (monomer) glycidyl methacrylate GMA 1.2960g, (crosslinking agent) ethylene glycol dimethacrylate EDMA 0.8640g, (initiator) benzyl peroxide Acyl BPO 0.0216g, (porogen) dodecanol 0.5184g and cyclohexanol 2.7216g are poured into a straight plastic mold with a length of 8cm and a diameter of 1.5cm, and blow nitrogen until the BPO is completely dissolved. Put i...

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Abstract

The invention discloses a method for carrying out surface-enhancement Raman spectrum detection on the surface of a cellular material. The method comprises the steps of: a) attaching an analyte combined with metal nanoparticles to the surface of the cellular material; or b) attaching the metal nanoparticles to the surface of the cellular material first, and then combining the analyte with the metal nanoparticles attached to the surface of the cellular material; and c) then, detecting a surface-enhancement Raman signal of the surface of the material obtained in the step a) or step b). According to the method for carrying out surface-enhancement Raman spectrum detection on the surface of the cellular material provided by the invention, the cellular material and the metal nanoparticles are combined as an enhanced substrate, so that the Raman signal of the analyte is extremely enhanced, therefore, surface-enhancement Raman ultra-sensitive detection or even detection of monomolecular tested substance can be realized. The detection concentration is low to 10-18mol / L, the quantity demanded for a sample of the analyte is less, and microlitres of the sample are required, so that the detection of trace substances can be met.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis, in particular to a method for surface-enhanced Raman spectrum detection on the surface of a porous material. Background technique [0002] Since Fleischmann, Van Duyne and Creighton discovered and confirmed the surface-enhanced Raman scattering (SERS) phenomenon in 1974, after decades of development, SERS technology has gradually become a A very active research field with wide applications in chemistry, catalysis, polymers, surface science, life science, etc. [0003] Many compounds can produce SERS effect, including inorganic molecules, organic molecules, and even macromolecules. The most studied are heterocyclic compounds such as pyridine, such as picoline, methyl violet, bipyridine, piperidine, pyrazine, and cyanopyridine, which generally have strong SERS effects. The SERS spectra of some dyes, metal complexes, biomolecules, and inorganic molecules have also been extensively studie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 杜一平李青青唐慧容汪宣陈贵平吉布仁・伊克巴尔张维冰
Owner EAST CHINA UNIV OF SCI & TECH