Molecular marker for quickly increasing green shell rate of duck groups

A technology of molecular markers and populations, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as slowing down the progress of duck eggshell color screening

Active Publication Date: 2020-01-17
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no relevant molecular marker for screening duck eggshell color, which greatly slows down the screening progress of duck eggshell color

Method used

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  • Molecular marker for quickly increasing green shell rate of duck groups
  • Molecular marker for quickly increasing green shell rate of duck groups
  • Molecular marker for quickly increasing green shell rate of duck groups

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1, Determination of the correlation between SNP molecular markers and eggshell color traits

[0088] Experimental materials: Peking duck M1 population female ducks (total 400) bred in Changping Breeding Base of Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences.

[0089] 1. Determination of eggshell color properties

[0090] The female ducks in the M1 group were raised until the egg-laying period, and each duck was transferred to an individual egg-laying cage for feeding before laying. During the egg-laying period, the eggshell color of each duck egg in each cage was recorded continuously for 5 days, and the eggshell color of each duck egg was finally determined. Eggshell color traits in female ducks.

[0091] 2. Genome Sequencing

[0092] 1. Blood sample collection: Use heparin sodium anticoagulant blood collection tubes to collect the venous blood of the duck wings to be tested, and store it at -20°C for l...

Embodiment 2

[0097] Example 2. Design of primers for identifying the nucleotide polymorphism at chr4:47418074

[0098] According to the whole genome sequencing results of Example 1, the primers for identifying the nucleotide polymorphism at the chr4:47418074 site on the duck genome were designed as follows:

[0099] Upstream primer: 5'-CGAGGCAGGGGACCTATCTT-3' (SEQ ID No.1);

[0100] Downstream primer: 5'-CTGTGCAGACTAAGAGGCGT-3' (SEQ ID No. 2).

[0101] For the situation that the nucleotide of chr4:47418074 site on the duck genome (i.e. the 507th deoxyribonucleotide of SEQ ID No.3) is A homozygote (recorded as A:A genotype), the theoretical amplification product There is only one DNA fragment shown in SEQ ID No.3 whose 507th deoxyribonucleotide is A.

[0102] For the situation of G homozygosity (denoted as G:G genotype) for the nucleotide of chr4:47418074 site (i.e. the 507th deoxyribonucleotide of SEQ ID No.3) on the duck genome, the theoretical amplification product There is only one D...

Embodiment 3

[0104] Example 3. Separation of M1 populations of Peking ducks according to genotypes to establish M1 white shell base populations and M1 green shell base populations respectively

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Abstract

The invention discloses a molecular marker for quickly increasing the green shell rate of duck groups and provides application of a substance for detecting whether 47418074th deoxyribonucleotide on anumber 4 chromosome in duck genome is A, G or A and G to identification of color traits of to-be-tested duck egg shells. By whole genome sequencing of a great quantity of duck samples, egg shell colorrelated SNP is researched, and specific SNP is discovered. Screening of green shell / white shell traits of laying ducks can be realized on the basis of genotypes of the SNP site, the genotypes of thegreen shell trait are AA and AG, and the genotype of the white shell trait is GG. By adoption of the molecular marker, quick purification of egg shell colors can be realized, a purified state can be achieved only through one-generation breeding, and early selection of the egg shell color traits can be realized. In addition, production cost can be saved while genetic progress is accelerated to better serve duck breeding, and high economic application values and scientific research values are achieved.

Description

technical field [0001] The invention relates to a molecular marker-assisted selection technology in the technical field of animal genetics and breeding, in particular to a molecular marker and its application for rapidly increasing the green shell rate of duck populations. Background technique [0002] my country's laying duck market and breeding scale are developing rapidly, and the species, quantity and output of breeding are all in the forefront of the world. Green-shelled duck eggs have high eggshell strength and are not easily damaged during processing. They are deeply loved by consumers. The breeding of green-shelled duck strains is a major demand for the industry. [0003] The study found that the eggshell color trait of poultry can be inherited stably, and the green shell trait of multiple poultry strains is determined by a dominant single gene. In poultry green-egg breeding, it is difficult to eliminate recessive genes by selecting the phenotype of female green-egg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 侯水生胡健刘贺贺周正奎郭占宝
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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