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Sensor surface for surface plasmon resonance assays

A sensor, ligand technology, applied in the field of production and its use in SPR assay or interaction, can solve the problems of high background noise and non-specific binding

Pending Publication Date: 2020-01-17
CYTIVA SWEDEN AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] However, depending on the type of sample, several currently known matrices have several disadvantages, such as high background noise and non-specific binding

Method used

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  • Sensor surface for surface plasmon resonance assays
  • Sensor surface for surface plasmon resonance assays
  • Sensor surface for surface plasmon resonance assays

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Embodiment Construction

[0036] In preliminary studies, the present inventors have found that self-assembled monolayers (SAM) formed from oligo(ethylene glycol) (OEG)-derived alkanethiol compounds or mixtures of these compounds with alkanethiol compounds without OEG Shows high non-specific binding. Results from cyclic voltammetry analysis were poor, indicating unfavorable packing density and poor surface coverage, thus a high probability of unwanted and non-specific binding of the sample to the gold surface / damaged SAM. This result suggests that SAM alkanethiol-OEG-containing surfaces are not suitable for high-sensitivity applications.

[0037] The present invention provides a novel sensor surface in which the capture molecules will be tightly bound to the sensor surface without binding to any matrix molecules to minimize baseline drift. The capture molecules are bound directly to the alkanethiol SAM layer (or via a linker on the SAM), not via PEG or OEG or any other hydrophilic or protein resistant ...

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Abstract

The present invention relates to a method for production of an improved sensor surface for an SPR instrument, comprising forming a self assembled monolayer (SAM) on a surface and attaching ligands andprotein resistant groups, preferably polyethylene glycol (PEG),directly to functional groups on said surface. The invention also relates to a sensor surface produced by these methods use thereof in SPR (surface plasmon resonance) assays or interactions.

Description

[0001] field of invention [0002] The present invention relates to a method and apparatus for assay improvement. More precisely, the present invention relates to the production of an improved sensor surface and its use in SPR (Surface Plasmon Resonance) measurements or interactions. The invention also relates to a sensor surface produced by these methods. [0003] Background of the invention [0004] Analytical sensor systems that can monitor interactions between molecules such as biomolecules in real time are gaining increasing interest. These systems are usually based on optical biosensors and are often referred to as interaction analysis sensors or biospecific instruments that use surface plasmon resonance (SPR) to detect interactions between molecules in a sample and molecular structures immobilized on a sensing surface. interaction. As the sample passes over the sensor surface, the progress of binding directly reflects the rate at which interactions occur. The sample ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/557
CPCG01N33/54373G01N33/54393G01N33/557G01N21/554G01N2610/00
Inventor L.温特巴克A.拉格尔P.谢林T.达尔摩
Owner CYTIVA SWEDEN AB