Immune lateral chromatography detection method and device for carrying out external drive on biomarker

Abstract

The invention discloses an immune lateral chromatography detection method and device for carrying out external drive on a biomarker. The device comprises immune lateral chromatography test paper consisting of a bottom liner 1, a sample pad 2, a combination pad 3, an analysis membrane 4 and a water absorption pad 5, wherein the analysis membrane is provided with a detection belt 6 and a quality control belt 7; the device further comprises an upstream drive electrode 8 and a downstream drive electrode 9; the upstream drive electrode is connected with the end part of the upstream outer side of the sample pad; the downstream drive electrode is connected with the end part of the downstream outer side of the water absorption pad. According to the method disclosed by the invention, strong and uniform external drive force generated by the electrodes is used as a power source to carry out external drive on surging of the biomarker, so that the precision, the accuracy and the detection speed ofvarious existing immune lateral chromatography technologies are greatly improved; on the basis, the detection sensitivity is improved through promoting release of the biomarker.

Description

[0001] This application is a divisional application with an application date of December 29, 2014, application number 201410833035.3, and the title of the invention "an immune lateral flow chromatography device and method for externally driving biomarkers". technical field [0002] The invention belongs to the technical field of biological detection in in vitro diagnosis, and relates to an immune lateral flow detection method and device for externally driving biological markers. Background technique [0003] Immunolateral flow chromatography is one of the most classic on-site rapid detection methods in the field of in vitro diagnostic technology. The physical composition of the immunological lateral flow test paper includes three types of substances: biologically active molecules, markers, and solid-phase consumables. Its specific components and functions are as follows: [0004] Solid-phase consumables: a variety of microporous materials, which can be used as sample pads, ...

AI Technical Summary

Problems solved by technology

However, the production process of solid-phase consumables for immunological lateral flow chromatography determines that there must be great inhomogeneity in their microscopic pore size and distribution state, which also leads to the problem that current chromatography products face large variations in test results; at the same time, weak The strong capillary siphon force also makes the time required for lateral chromatography relatively long (about 10-15 minutes)

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