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A method for evaluating the cross-compatibility between transgenic soybean and wild soybean

A technology of genetically modified soybeans and wild soybeans, applied in botany equipment and methods, plant genetic improvement, application, etc., can solve problems such as unscientific compatibility, different risks of planting genetically modified soybeans, differences in pod setting rate and seed setting rate, etc.

Active Publication Date: 2021-04-06
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to differences in the compatibility between genetically modified soybeans and wild soybeans with different genetic backgrounds, the risk of planting genetically modified soybeans in different production areas is different under the condition of overlapping flowering periods; There are also significant differences in the pod setting rate and seed setting rate under the conditions, and it is not scientific to use the pod setting rate and seed setting rate alone to evaluate the affinity

Method used

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  • A method for evaluating the cross-compatibility between transgenic soybean and wild soybean
  • A method for evaluating the cross-compatibility between transgenic soybean and wild soybean
  • A method for evaluating the cross-compatibility between transgenic soybean and wild soybean

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Such as figure 1 It can be seen that the pods formed by selfing of wild soybeans are significantly different from the pods formed by transgenic soybean pollen. The pods formed by selfing are full, while the pods formed by crossing transgenic soybeans with wild soybeans are all shrunken. state, the number of full pods is very small, which is caused by the incompatibility between the two, so it is extremely important to establish a method for evaluating the compatibility of transgenic soybeans and wild soybean hybrids.

[0040] Such as figure 2 As shown, there are obvious differences in morphology between the seeds produced by crossing transgenic soybeans and wild soybeans and the seeds produced by selfing of wild soybeans. Most of the seeds formed are full grains. This resulted in a very significant difference in the index of the number of full grains between the two, so the full grain ratio of the two is extremely critical for us to evaluate their hybrid compatibilit...

Embodiment 2

[0057] Compatibility Evaluation of Glyphosate-resistant Transgenic Soybean and 18 Wild Soybean Populations

Embodiment approach

[0059] Collection of experimental materials: glyphosate-resistant transgenic soybeans were provided by the National Soybean Improvement Center of Nanjing Agricultural University. Wild soybean populations were collected in 18 different geographical locations across the country, see Table 1.

[0060] Table 1 Basic information of tested wild soybean populations

[0061]

[0062] Flowering meet planting technology: On the basis of fully understanding the growth characteristics of genetically modified soybeans and wild soybeans, the genetically modified soybeans and wild soybeans are planted in batches. The sowing container is a plastic flowerpot with holes at the bottom, and the planting substrate is farmland soil and organic cultivation soil. 1:1 mix well. One wild soybean plant was planted in each pot, and 8 plants constituted a repetition, a total of 4 repetitions, and one batch was planted. In order to ensure that wild soybeans can meet the flowering period of glyphosate-...

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Abstract

The invention belongs to the field of ecological safety assessment of transgenic soybeans, and in particular relates to a method for evaluating the hybrid compatibility of transgenic soybeans and wild soybeans, and counting the number of full-grained seeds produced by crossing transgenic soybeans with male parents and wild soybeans under the same growth environment conditions The hybrid compatibility between transgenic soybeans and wild soybeans was evaluated by the ratio of full seeds to the number of full seeds podded in the wild soybean self-cross. The specific test operation steps include the planting technology of meeting at the flowering stage, the thorough detasseling technology, the successful pollination technology, the self-fertilization bud determination technology, the data statistics method, and the compatibility evaluation technology. The invention proposes the technology and evaluation standard for the implementation of the hybrid affinity evaluation test of transgenic soybeans and wild soybeans, which plays an important role in environmental risk management and prevention when transgenic soybeans are safely put into production. The invention has the advantages of strong test operability, simple data processing, objective results, high accuracy and the like.

Description

technical field [0001] The invention relates to a method for evaluating the hybrid compatibility of transgenic soybeans and wild soybeans, and belongs to the field of ecological safety evaluation of transgenic soybeans. Background technique [0002] Genetically modified crops were commercially grown in 1996. By 2018, biotech crops had grown to 191.7 million hectares. Among all genetically modified crops, the planting area of ​​Glycine max is the largest, reaching 95.9 million hectares in 2018, accounting for 50% of the global planting area of ​​genetically modified crops, and all the planted genetically modified soybeans contain herbicide resistance genes. In recent years, China's soybean imports have continued to grow, reaching 95.53 million tons in 2017. China is the main distribution area of ​​wild soybean resources. In order to prevent foreign genes from contaminating wild soybean resources, it is urgent to conduct a systematic assessment of the risk of gene drift that...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 宋小玲强胜胡玉琪盛泽文刘金悦刘琪
Owner NANJING AGRICULTURAL UNIVERSITY