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Preparation method of magnetic microspheres with fluffy shell

A technology of magnetic microspheres and shell layers, applied in the preparation of microspheres, microcapsule preparations, etc., can solve the problems of wide particle size distribution, complex preparation process, uneven particle size, etc., and achieve wide particle size distribution and technological process. Simple and convenient effects

Active Publication Date: 2021-09-03
SUZHOU WEIDU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Magnetic microspheres can be prepared by a variety of methods, such as chemical precipitation and monomer polymerization, but these methods have certain limitations, including particle size inhomogeneity, wide particle size distribution, complex preparation process, magnetic leakage, etc. and other shortcomings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Weigh 10g of polystyrene microsphere (500nm) solution (10wt%) after magnetic separation, remove the supernatant liquid and disperse in 100ml of acetonitrile solution, then add 5g of acrylic acid, 0.25g of N,N-methylenebis acrylamide and 0.1 g of azobisisobutyronitrile. The whole solution was ultrasonicated on a cell pulverizer for 5 minutes, then poured into a three-necked flask, stirred evenly, heated to 70° C., and reacted for 5 hours to obtain polystyrene microspheres coated with a carboxyl shell.

[0022] The obtained polystyrene microspheres coated with carboxyl shell were centrifuged and washed three times with absolute ethanol and deionized water respectively to obtain carboxy polystyrene microspheres with core-shell structure, which were loaded into centrifuge tubes spare.

[0023] If you want to adjust the bulkiness of the shell, you can slowly add an acid solution or an alkali solution to the solution, and the pH value can be adjusted in the range of 4-11, or...

Embodiment 2

[0027] Weigh 10g of polystyrene microsphere (900nm) solution (10wt%) after magnetic separation, remove the supernatant liquid and disperse in 100ml of acetonitrile solution, then add 5g of acrylic acid, 0.25g of N,N-methylenebis acrylamide and 0.1 g of azobisisobutyronitrile. The whole solution was ultrasonicated on a cell pulverizer for 5 minutes, then poured into a three-necked flask, stirred evenly, heated to 70° C., and reacted for 5 hours to obtain polystyrene microspheres coated with a carboxyl shell.

[0028] The obtained polystyrene microspheres coated with carboxyl shell were centrifuged and washed three times with absolute ethanol and deionized water respectively to obtain carboxy polystyrene microspheres with core-shell structure, which were loaded into centrifuge tubes spare.

[0029] If you want to adjust the bulkiness of the shell, you can slowly add acid solution or alkali solution to the solution, and the pH value can be adjusted in the range of 4-11.

[0030...

Embodiment 3

[0033] Weigh 10g of polystyrene microsphere (5um) solution (10wt%) after magnetic separation, remove the supernatant liquid and disperse in 100ml of acetonitrile solution, then add 10g of methacrylic acid, 0.5g of divinylbenzene and 0.2 g of azobisisobutyronitrile. The whole solution was ultrasonicated on a cell pulverizer for 5 minutes, then poured into a three-necked flask, stirred evenly, heated to 70° C., and reacted for 5 hours to obtain polystyrene microspheres coated with a carboxyl shell.

[0034] The obtained polystyrene microspheres coated with carboxyl shell were centrifuged and washed three times with absolute ethanol and deionized water respectively to obtain carboxy polystyrene microspheres with core-shell structure, which were loaded into centrifuge tubes spare.

[0035] If you want to adjust the bulkiness of the shell, you can slowly add acid solution or alkali solution to the solution, and the pH value can be adjusted in the range of 4-11.

[0036] Add 0.5mo...

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Abstract

The present invention relates to a preparation method of magnetic microspheres with fluffy shells. 1) Synthesis of carboxyl shells: disperse seed microspheres in an organic solvent, then add carboxyl monomers, initiators and crosslinking agents, and heat in a water bath to a certain temperature and react for a certain period of time to obtain microspheres coated with carboxyl shells. 2) Preparation of amino magnetic particles: Weigh a certain amount of iron salt and dissolve it in a certain amount of deionized water. After stirring for a certain period of time, add an alkaline solution, and react for a certain period of time to obtain magnetic particles. After magnetic separation and washing, disperse in polyacrylamide , to obtain amino magnetic particles. 3) Preparation of magnetic microspheres: Mix a certain concentration of a polymer microsphere solution with a carboxyl shell and a certain concentration of an amino magnetic particle solution in a certain ratio, and stir overnight to obtain magnetic microspheres. The thickness of the shell layer can be adjusted and controlled by changing the feeding amount of carboxyl monomer; and the fluffy degree of the shell layer can also be changed by adjusting the ratio of carboxyl monomer and crosslinking agent; the whole process is simple and convenient.

Description

technical field [0001] The invention relates to a preparation method of magnetic polymer microspheres, in particular to a preparation method of magnetic microspheres with a fluffy shell. Background technique [0002] The surface shell of magnetic particles can be modified by different groups or recognition components, such as hydroxyl, amino, carboxyl, streptavidin, biotin and different antibodies. These groups and recognition components that can bind to biomolecules endow the surface functionalization of magnetic particles. With the close combination of magnetic nanotechnology and biomedicine, functionalized micro-nano magnetic particles have been widely used in the field of biomedicine, such as the separation and purification of nucleic acids and proteins, the separation and detection of cells, gene and drug delivery carriers and contrast agents, etc. . [0003] Magnetic microspheres can be prepared by a variety of methods, such as chemical precipitation and monomer poly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/14
CPCB01J13/14
Inventor 刘照关杨永哲杜德状
Owner SUZHOU WEIDU BIOTECH CO LTD