Method for separating cannabidiol by using continuous chromatographic system

A continuous chromatography and cannabidiol technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve problems such as unfavorable industrial production, large environmental pollution, and high solvent consumption, and achieve low labor intensity and high product quality. The effect of high recovery rate and reduction of production cost

Pending Publication Date: 2020-02-04
河南汉麻生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the above problems, the present invention provides a method for separating cannabidiol using a continuous chromatographic system, which avoids the problems of much solvent consumption, high cost, large environmental pollution, and unfavorable i

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  • Method for separating cannabidiol by using continuous chromatographic system

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Effect test

Embodiment 1

[0032] A method for separating cannabidiol utilizing a continuous chromatographic system, said method comprising the steps of:

[0033] Step 1, reflux extraction:

[0034] Take marijuana flowers and leaves, crush them and extract them under heat reflux with an ethanol solution with a mass concentration of 60%, and then concentrate the obtained extract under reduced pressure to obtain a concentrated liquid extract; the total content of cannabidiol and tetrahydrocannabinol is 15%. 12% cannabidiol and 3% THC;

[0035] Step 2. Configure the solution:

[0036] Dissolve the concentrated liquid extract obtained in step 1 in an ethanol mobile phase with a mass fraction of 65%, and configure it as a raw material solution with a concentration of 100 mg / mL;

[0037] Step three, separation:

[0038] The mobile phase described in step 2 and the obtained raw material solution are continuously sent into the continuous chromatography system, and the cannabidiol solution is continuously col...

Embodiment 2

[0046] A method for separating cannabidiol utilizing a continuous chromatographic system, said method comprising the steps of:

[0047] Step 1, reflux extraction:

[0048] Take marijuana flowers and leaves, crush them and extract them under heat reflux with an ethanol solution with a mass concentration of 65%, and then concentrate the obtained extract under reduced pressure to obtain a concentrated liquid extract; the total content of cannabidiol and tetrahydrocannabinol is 15%, 12% cannabidiol and 3% THC;

[0049] Step 2. Configure the solution:

[0050] Dissolve the concentrated liquid extract obtained in step 1 in the acetone mobile phase with a mass fraction of 65%, and configure it as a raw material solution with a concentration of 300 mg / mL;

[0051] Step three, separation:

[0052]The mobile phase described in step 2 and the obtained raw material solution are continuously sent into the continuous chromatography system, and the cannabidiol solution is continuously col...

Embodiment 3

[0060] A method for separating cannabidiol utilizing a continuous chromatographic system, said method comprising the steps of:

[0061] Step 1, reflux extraction:

[0062] Take marijuana flowers and leaves, crush them and extract them under reflux with ethanol solution with a mass concentration of 100%, and then concentrate the obtained extract under reduced pressure to obtain a concentrated liquid extract; the total content of cannabidiol and tetrahydrocannabinol is 15%, 12% cannabidiol and 3% THC;

[0063] Step 2. Configure the solution:

[0064] The concentrated liquid extract obtained in step 1 is dissolved in the acetonitrile mobile phase with a mass fraction of 55%, and is configured into a raw material solution with a concentration of 600mg / mL;

[0065] Step three, separation:

[0066] The mobile phase described in step 2 and the obtained raw material solution are continuously sent into the continuous chromatography system, and the cannabidiol solution is continuousl...

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Abstract

The invention discloses a method for separating cannabidiol by using a continuous chromatographic system. The method comprises the following steps: extracting a raw material cannabis flowers and leaves with ethanol, then performing concentrating to obtain an extract, dissolving the extract rich in cannabidiol by using a mobile phase to make the concentration reach 10-600 mg/mL, feeding the obtained raw material solution and the mobile phase into the continuous chromatographic system through a raw material pump and an elution pump, continuously collecting a cannabidiol solution from the raffinate port of the continuous chromatographic system, continuously collecting a tetrahydrocannabinol solution from an extraction port, concentrating and crystallizing the obtained cannabidiol solution toobtain a dry cannabidiol powder with the purity of 99.0% or above, and destroying the dry cannabidiol powder after the tetrahydrocannabidiol is concentrated, wherein a filler used by the continuous chromatographic system is a polymer filler. The purification method has the advantages of high yield, simple process, low solution consumption, high filler utilization rate, and suitableness for industrial production.

Description

technical field [0001] The invention relates to the technical field of cannabidiol separation, in particular to a method for separating cannabidiol by using a continuous chromatographic system. Background technique [0002] Cannabis (scientific name: Cannabissativa L.) Moraceae, Cannabis genus, annual erect herb, 1-3 meters high. Branches with longitudinal grooves, densely gray-white appressed hairs. Leaves palmately split, lobes lanceolate or linear-lanceolate, especially the dried flowers and trichomes of female plants. Cannabis can be peeled and harvested. There are females and males. The male plant is called Ju, and the female plant is called Ju. Cannabidiol (CBD) is a monomer extracted from cannabis. It is the first cannabinoid drug approved by the US FDA. Studies have shown that cannabidiol has important clinical application prospects, such as: anti-spasmodic, anti-anxiety , anti-inflammatory, anti-oxidation and other pharmacological effects, it has certain curati...

Claims

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Application Information

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IPC IPC(8): C07C37/68C07C37/70C07C37/82C07C37/84C07C39/23
CPCC07C37/004C07C37/685C07C37/70C07C37/82C07C37/84C07C2601/16C07C39/23
Inventor 郭二影张金成朱晓彤
Owner 河南汉麻生物科技有限公司
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