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Method for investigating molecules such as nucleic acids

A nucleic acid analysis and molecular technology, applied in the field of molecular research such as nucleic acid, can solve the problem of the limitation of the degree to which the speed at which droplets can move can be changed

Inactive Publication Date: 2020-02-11
BASE4 INNOVATION LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Additionally, there are limits to how fast a droplet can move and how much the actual droplet path can change

Method used

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  • Method for investigating molecules such as nucleic acids
  • Method for investigating molecules such as nucleic acids
  • Method for investigating molecules such as nucleic acids

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Embodiment Construction

[0068] The method of the invention suitable for sequencing a DNA analyte according to a preferred third aspect of the invention is now illustrated by the following figures and the associated description.

[0069] figure 1 shows a top view of a microfluidic substrate comprising a droplet preparation zone 1 and a droplet manipulation zone 3 integrated into a single substrate. 1 includes regions 7, 8 and 9 containing fluids attached to inlets 4, 5 and 6 which respectively introduce into the substrate a flow of pyrophosphate hydrolysis, a flow of inorganic pyrophosphatase, and various Detects the flux of chemicals and enzymes required to identify nucleoside triphosphates according to one of our earlier patent applications. These streams are then delivered to orifices 7a, 8a and 9a, respectively, from which various droplets 7b, 8b and 9b are produced (eg, using a droplet dispense head or by cutting out from larger intermediate droplets). 10 is a reservoir containing paramagnetic...

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Abstract

A method for manipulating a microdroplet of a reaction medium in an immiscible carrier medium with a target molecule bound to a solid support for the purposes of effecting a chemical transformation isprovided. It is characterised by the steps of (a) bringing the microdroplet into contact with the solid support under conditions where the microdroplet and solid support are caused to combine, (b) allowing the reaction medium to react with the target molecule and (c) thereafter exerting a force to induce the reaction medium to become detached from the solid support and reform a microdroplet in the carrier fluid. In one embodiment the solid support is a particle, bead or the like.

Description

technical field [0001] In particular, the invention relates to a method of studying nucleic acid molecules; in particular a method for sequencing DNA or RNA of natural or synthetic origin. Background technique [0002] In our previous applications WO 2014 / 053853, WO 2014 / 053854, WO2014 / 167323, WO2014 / 167324 and WO2014 / 111723, we have described a novel sequencing method involving stepwise digestion of polynucleotide analytes ( polynucleotide analyte) to produce an ordered stream of single nucleotides, preferably single nucleoside triphosphates, each of which can be captured one by one into the corresponding in the droplet. Thereafter, each droplet can be chemically and / or enzymatically treated to reveal the specific single nucleotide it originally contained. In one embodiment, these chemical and / or enzymatic treatments comprise a method involving the use of one or more multi-component oligonucleotide probe types (multi-component oligonucleotideprobe types), each type being ...

Claims

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Application Information

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IPC IPC(8): B01L3/00C12Q1/6869
CPCB01L3/502792C12Q1/6869B01L2200/0673B01L2300/0864B01L2300/0887B01L2300/089B01L2300/161B01L2300/165B01L2300/168B01L2400/0427B01L3/502784
Inventor 卡梅伦·亚历山大·弗雷林佩德罗·昆哈托马斯·亨利·艾萨克
Owner BASE4 INNOVATION LTD
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