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Lactobacillus plantarum and application thereof in preparation of miscanthus silage

A technology of Lactobacillus plantarum and Lactobacillus, applied in the field of microbiology, can solve the problems of high breeding cost, low digestibility, difficulty in silage of Miscanthus silage, etc., and achieve the effects of improving sensory quality, increasing organic acid content, and improving palatability

Active Publication Date: 2020-02-14
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide a kind of Lactobacillus plantarum and its application in the preparation of Miscanthus silage, to solve the difficulties of Miscanthus silage in the prior art, low digestibility, and high breeding costs.

Method used

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  • Lactobacillus plantarum and application thereof in preparation of miscanthus silage
  • Lactobacillus plantarum and application thereof in preparation of miscanthus silage
  • Lactobacillus plantarum and application thereof in preparation of miscanthus silage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified. The miscanthus variety used in the following examples for silage preparation is Nandi, which can be obtained from the variety owner and can only be used to repeat the experiment of the present invention.

[0020] Lactobacillus plantarum (ATCC 10241), Lactobacillus casei (JCM4926) and Lactobacillus casei (ATCC14435) purchase website http: / / www.biofeng.com / junzhu / biaozhunjunzhu;

[0021] (1) Rapid preparation of Lactobacillus mixed bacterial agent

[0022] 1) MRS medium (1L)

[0023] Liquid: peptone 10g, yeast extract powder 5g, K 2 HPO 4 2g, diammonium hydrogen citrate 2g, sodium acetate 5g, glucose 20g, beef extract 10g, MgSO 4 ·7H 2 O 0.58g, MnSO 4 ·H 2 O 0.25g, Tween 80 1ml;

[0024] Solid: agar 15g, CaCO 3 10g

[0025] Preparation method: Add the above ingredients into a triangular flask, add 1L of water, and stir and dissolve on a...

Embodiment 2

[0038] The added microbial agent of this embodiment is divided into four groups, is respectively the Lp-EC018 group that only adds Lactobacillus plantarum EC018, the L.casei group that only adds Lactobacillus casei (JCM4926), and the MIX1 group in Example 1 , the MIX2 group that added the mixture of Lactobacillus plantarum EC018 and Lactobacillus casei (JCM4926) (the ratio of the two strains is 1:1) at the same time, and its concentration was 10 9 cfu / ml, the silage method is the same as in Example 1.

[0039] The silage prepared from miscanthus as raw material of each embodiment is evaluated:

[0040] 1. Sensory evaluation

[0041] Table 1 Sensory evaluation of silage materials

[0042]

[0043] It can be seen from the above table that the microbial bacterial agent provided by the present invention can effectively improve the sensory image of miscanthus fermented, and greatly improve the appearance and appearance of Miscanthus forage after silage.

[0044] 2. Comparison o...

Embodiment 3

[0049] The microbial agent added in this embodiment is a mixture of Lactobacillus plantarum EC018 and Lactobacillus casei (ATCC144 35) (the ratio of the two strains is 1:1), and the concentration is 10 9 cfu / ml is defined as the MIX3 group, and all the other silage methods are the same as in Example 1.

[0050] The silage prepared from miscanthus as raw material of each embodiment is evaluated:

[0051] 1. Sensory evaluation

[0052] Table 3 Sensory evaluation of silage materials

[0053]

[0054] It can be seen from the above table that the microbial bacterial agent provided by the present invention can effectively improve the sensory image of miscanthus fermented, and greatly improve the appearance and appearance of Miscanthus forage after silage.

[0055] 2. Comparison of silage quality

[0056] Table 4 Comparison of Miscanthus silage quality under different microbial additives

[0057]

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Abstract

The invention discloses lactobacillus plantarum and application thereof in preparation of miscanthus silage, and belongs to the field of microorganisms. The lactobacillus plantarum EC018 is obtained by carrying out heavy ion radiation mutagenesis on lactobacillus plantarum, and is preserved in China General Microbiological Culture Collection Center on October 8th, 2018, and the preservation numberis CGMCC NO. 16556. The lactobacillus plantarum EC018 and lactobacillus casei are mixed and sprayed on the surface of miscanthus section in a thallus quantity ratio of 1:1, the pH value of the silagecan be reduced, the content of soluble carbohydrates and proteins in the fermented miscanthus feed can be increased, the content of ammoniacal nitrogen can be reduced, the degradation of proteins inthe feed can be prevented, the content of neutral detergent fibers and acidic detergent fibers in the feed can be reduced, the nutritional quality of the feed can be improved, and the feeding value ofthe feed can be increased; and meanwhile, the content of organic acid in the feed is increased, the palatability of the feed is improved, and the miscanthus becomes available silage.

Description

technical field [0001] The invention belongs to the field of microbes, in particular to a plant lactobacillus and its application in the preparation of Miscanthus silage. Background technique [0002] Miscanthus is a perennial C4 plant with the advantages of fast growth, high photosynthetic efficiency, high biomass, low water and fertilizer requirements, and few pests and diseases. Nandi is a unique miscanthus species in my country. It not only has all the excellent qualities of miscanthus, but also its young stems and leaves have high nutritional value, contain high concentrations of water-soluble carbohydrates and crude protein, and the total effective nutrient content is as high as 55%. %, very suitable for development as silage. At present, the lack of high-quality forage resources in my country has become a bottleneck restricting the development of animal husbandry. In order to speed up the development of animal husbandry, it has become an urgent task to develop and exp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/01C12N13/00A23K30/18C12R1/25
CPCA23K30/18C12N13/00C12N15/01C12R2001/25C12N1/205A23V2400/169
Inventor 周功克贺郭胡瑞波何康
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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