Kit, device, and method for lung cancer detection
A kit and technology for lung cancer, applied in the direction of measuring devices, biochemical equipment and methods, biochemical cleaning devices, etc., can solve the problem of heavy biopsy
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Embodiment 1
[1473]
[1474] In this example, using the learning sample group (b1 in Table 11) including lung cancer patients and subjects without lung cancer, after making a discriminant formula using one or two gene markers, the verification sample group (Table 11) In b2) of 11, the discriminant performance is evaluated, and genes used in the discriminant formula with high performance are extracted to obtain gene markers capable of detecting lung cancer.
[1475] Specifically, firstly, the miRNA expression levels of the learning sample group and the verification sample group obtained in the above reference example were combined, and normalized by global normalization. Furthermore, in order to obtain more reliable diagnostic markers, in any group of lung cancer patients or non-lung cancer subjects, only genes with 2 to the 6th power or more in more than 50% of the samples 396 genes with expression levels were used as analysis objects.
[1476]Next, Fisher discriminant analysis was perf...
Embodiment 2
[1510]
[1511] In this example, using a learning sample group (b1 in Table 11) including lung cancer patients and subjects without lung cancer, a discriminant formula using 1 to 5 gene markers is prepared, and then in the verification sample group (Table 11 In b2) of 11, the discriminant performance is evaluated, and genes used in the discriminant formula with high performance are extracted to obtain gene markers capable of detecting lung cancer.
[1512] Specifically, firstly, the miRNA expression levels of the learning sample group and the verification sample group obtained in the above reference example were combined, and normalized by global normalization. Furthermore, in order to obtain more reliable diagnostic markers, in any group of positive sample group (lung cancer patients) or negative sample group (healthy people, patients with benign bone and soft tissue tumors and benign breast diseases, and cancer patients other than lung cancer) Among them, only 396 genes wi...
Embodiment 3
[1554]
[1555] In this example, the expression levels of miRNAs in the serum of lung cancer patients and healthy subjects were compared to verify the gene markers obtained in Examples 1 and 2. In this case, the greater the number of samples, the greater the reliability of the statistical quantity of gene expression. Therefore, all the samples (a of Table 11) in which the gene expression was measured in the above reference example were used. Specifically, first, the miRNA expression levels of 1694 lung cancer patients and 4660 healthy people obtained from the above-mentioned reference example were combined and normalized by global normalization. Next, in order to evaluate more reliable diagnostic markers, in either the lung cancer patient group or the normal group, only genes with a gene expression level of 2 to the power of 6 or more were selected in 50% or more of the samples. Furthermore, in order to evaluate genes with statistically significant differences in the gene ex...
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