A kind of bulbous bud propagation method of revive plant Selaginella cuscus
A technology of Selaginella cuspidatum and recovery plants, applied in the field of plant propagation, can solve the problems of low natural fertility, reduction of wild population of Selaginella cuspidatum of recovery plants, difficulty in cutting propagation, etc., and achieve the effect of high colonization survival rate
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Embodiment 1
[0027] Embodiment 1 The method of the present invention
[0028] (1) Induction culture of Selaginella cuspidatum
[0029] The 3-year-old single plant (with roots) of wild Selaginella serrata was planted in a plastic flowerpot equipped with a mixed substrate for inducing culture of pearl buds, the inner diameter of the flowerpot was 15-16 cm, and the mixed substrate was composed of peat and perlite in a volume ratio= 10:1 mixed with 2kg / m added to the mixed matrix 3 The slow-release fertilizer, in terms of mass fraction, the slow-release fertilizer contains N14%, P 2 O 5 14%, K 2 O14% and S 4%. The flowerpots planted with the cushion-shaped selaginella plants are placed in a low-light greenhouse equipped with shading nets, supplementary lights and temperature control equipment. ~180μmol m -2 ·s -1 , the light intensity at noon on cloudy days and noon on rainy days is 40-60 μmol m -2 ·s -1 . Water every 4 days to keep the water content of the mixed substrate at 40-45%...
Embodiment 2
[0039] Embodiment 2 and embodiment 3 are methods of the present invention
[0040] Except for the different measures listed in Table 1, the other measures in Example 2 and Example 3 are the same as those in Example 1, and will not be repeated here.
[0041] Table 1 Differences between the steps of Example 2-Example 3 and Example 1
[0042]
[0043]
[0044] The reproductive effect of table 2 embodiment 1-3
[0045]
[0046] control
[0047] The control was the reproduction of the wild plants of Selaginella cuspidatum in the wild habitat.
[0048] (1) Observation objects: In the same growing area in the field, 30 2-3 year-old wild plants of Selaginella cuspidatum with the same growth pattern were selected as the observation group.
[0049] (2) Wild habitat: bare limestone surface, extremely thin soil layer; distinct dry season and rainy season, the average annual precipitation is about 600-800 mm, of which the dry season precipitation accounts for 10-12%; the noon l...
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