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Panoramic stitching system and method for microscopic images

A technology of microscopic image and splicing system, which is applied in the field of microscopic image panoramic splicing system, can solve problems such as affecting image accuracy and increasing workload.

Active Publication Date: 2020-02-18
WUHAN LANDING INTELLIGENCE MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the lack of hardware support leads to a substantial increase in the workload of the program
For example, some relatively backward microscopes with manual displacement, or the inconsistent setting of the displacement direction and path by different types of microscopic scanning devices can easily lead to the scheme can only be sorted manually, resulting in a substantial increase in workload
Furthermore, there may be slight position differences in the images stitched by this scheme, which will affect the accuracy of the images

Method used

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  • Panoramic stitching system and method for microscopic images
  • Panoramic stitching system and method for microscopic images

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] Such as figure 1 As shown in , a system for panoramic stitching of microscopic images is characterized in that it includes: a field of view sub-block matching module, a field of view position fitting module and a block extraction module;

[0131] The field of view sub-block matching module is used to identify the overlapping area between each image and judge the adjacent positional relationship between the sub-images, so that each sub-image acquired by the microscopic scanning device is automatically arranged according to the order of the stitching of the images;

[0132] The field of view position fitting module is used to fine-tune the position according to the overlapping area between each sub-image, so that the cell position can be accurately stitched together;

[0133] The block extraction module is used to automatically extract the stitched complete image.

[0134] In the preferred solution, the operation process of the visual field sub-block matching module is: ...

Embodiment 2

[0187] A method for microscopic image panorama stitching, comprising the following steps:

[0188] S1. Field of view sub-block matching; the field of view sub-block matching module is used to identify the overlapping area between the images and judge the adjacent positional relationship between the sub-images, so that the sub-images acquired by the microscopic scanning device are automatically arranged according to the order of the images. ;

[0189] S2, field of view position fitting; the field of view position fitting module is used to fine-tune the position according to the overlapping area between each sub-image, so that the cell position can be accurately stitched together;

[0190] S3. Block extraction; the block extraction module is used to automatically extract a complete stitched image.

[0191] 6. A method for microscopic image panorama stitching according to claim 5, characterized in that: the operation process of field of view sub-block matching is as follows:

...

Embodiment 3

[0244] On the basis of embodiment 1~2, as Figure 5~6 In , take a cytopathological analysis example as an example: the images automatically acquired from the micro scanning device are as follows: Figure 5As shown in the figure above, the ordering of each sub-image is irregular, which depends on the automatic acquisition path of the micro-scanning device. During the acquisition process, it is ensured that each image has overlapping positions. Analyze the pixel values ​​at overlapping positions, automatically match the image to the corresponding position through the intelligent algorithm of field of view sub-block matching, and calculate the two-dimensional transformation matrix from the platform offset to the pixel offset according to the matched feature points in the adjacent field of view Initial value, to get splicing parameters. Specifically, each view sub-block is determined, that is, the adjacent position of the sub-image relative to other sub-images. Cut the common pa...

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Abstract

The invention provides a panoramic stitching system and method for microscopic images. The panoramic stitching system comprises a view sub-block matching module, a view position fitting module and a block extraction module, the view sub-block matching module is used for identifying an overlapping region between the images and judging an adjacent position relationship between the sub-images, so that the sub-images acquired by the microscopic scanning device are automatically arranged according to a splicing sequence of the images; the visual field position fitting module is used for finely adjusting the position according to the overlapping area between the sub-images so as to accurately splice the cell positions; and the block extraction module is used for automatically extracting a completely spliced image. By the adoption of the scheme, automatic splicing can be achieved, high-definition images can be obtained, in the splicing process, the method can adapt to disorderly-arranged images without clearly scanning the sequencing problem of the images, high compatibility is achieved, and labor intensity is greatly reduced. The splicing process of the method can be completed in a second-level range so as to adapt to the data processing intensity of large-scale diagnosis on the cloud.

Description

technical field [0001] The invention relates to the field of intelligent identification of cells by using computers, in particular to a system and method for panorama stitching of microscopic images. Background technique [0002] Cytological detection can quickly distinguish diseases according to the number and shape of cells, especially for the resolution and detection of tumor diseases. However, the efficiency of manual inspection in clinical practice is extremely low, and experienced doctors can only inspect about 50 samples per day. With the development of science and technology, automatic image analyzers have been used in cytopathological analysis. Can greatly improve efficiency. A high-magnification microscopic scanning device can generate multiple images with independent focal points for a sample. These images with independent focal points need to be stitched together. In the prior art, a combination of software and hardware is usually used for stitching, such as th...

Claims

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Application Information

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IPC IPC(8): G06T3/40G06T5/50
CPCG06T3/4038G06T5/50G06T2200/32G06T2207/10056G06T2207/20221G06T2207/30024G06T2207/20021Y02P90/30
Inventor 孙小蓉庞宝川肖笛
Owner WUHAN LANDING INTELLIGENCE MEDICAL CO LTD
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