Preparation method of amnion-spongy chitosan composite double-layer wound dressing

A composite bilayer and chitosan technology, applied in the fields of medical science, absorbent pads, tissue regeneration, etc., can solve the problem of difficult to take into account the rapid healing of wound exudate and wound recovery, and achieve the effect of rapid absorption and prevention of wound infection.

Inactive Publication Date: 2020-03-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes combining two different types of materials together: an amnion membranes made up mainly of proteins called laminarin or gelatin, alongwith other substances like polysaccharides such as glucans found naturally occurring within them. These composites have several advantages over single layers due to their unique structure and ability to create strong bonds between these components without losing any functionality they provide for biological activity. They also increase blood clotting time effectively on both sides of wounding sites compared to traditional methods involving multiple layers of material.

Problems solved by technology

The technical problem addressed in this patented text relates how to develop better ways for filling deeper wounds with effective substances like proteins or other nutrients while maintaining their effectiveness over longer periods without causing damage from bacteria or viruses.

Method used

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  • Preparation method of amnion-spongy chitosan composite double-layer wound dressing
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  • Preparation method of amnion-spongy chitosan composite double-layer wound dressing

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Embodiment 1

[0030] The optimization of the decellularization condition of embodiment 1 amniotic membrane

[0031] Four conditions were used to decellularize the amniotic membrane. Rinse the fresh amniotic membrane with deionized water, then soak the amniotic membrane in 0.25% or 0.5% trypsin solution, stir for 2 hours, wash it with deionized water, and treat it in 1% SDS aqueous solution for 12 hours or 24 hours. After treatment, the amniotic membrane was fully washed with deionized water, and after natural air drying, it was stained with DAPI. The results of DAPI staining are shown in figure 1 . By observing the amount of DNA residues, it can be found that when 0.5% trypsin is used for 2 hours, and then 1% SDS is used for 24 hours, the cells in the amniotic membrane are efficiently and thoroughly removed, so 0.5% trypsin is selected for 2 hours to combine 1% SDS was treated for 24 hours as the decellularization condition of the amnion.

Embodiment 2

[0032] The synthesis of embodiment 2 double-layer dressings

[0033] For the synthesis flow chart of double-layer dressing, see figure 2 . The specific steps are as follows: cut the decellularized amniotic membrane into a rectangle of 3 cm × 10 cm and place it in a mold, dissolve chitosan in 1% acetic acid aqueous solution to prepare a 20 mg / ml solution, and prepare amniotic membrane: chitosan: cross Add chitosan and cross-linking agent solution to the amniotic membrane at a mass ratio of 1:1:0.2 or 1:3:0.6 (m / m / m) with PEGDGE to prepare double-layered membranes M1-1 with different thicknesses, M1-3, after freezing at -80°C, freeze-dry. After lyophilization, the mold was transferred to an oven at 40°C, and the reaction was continued for 6 hours. Finally, a cross-linked double-layer dressing is obtained, and the finished product of the double-layer dressing is shown in image 3 . The prepared double-layer dressing was characterized by scanning electron microscopy, and the...

Embodiment 3

[0034] The effect of embodiment 3 different double-layer dressings on mouse wound healing

[0035] 0.1mol / L sodium citrate buffer solution: add 2.10g of citric acid to 100ml of double distilled water to make a citric acid mother solution, which is called liquid A; add 2.94g of trisodium citrate to 100ml of double distilled water to make a sodium citrate mother solution, It is called liquid B; mix liquids A and B at a ratio of 1:1.32 (v / v), and adjust the pH of the solution to 4.0.

[0036] Streptozotocin (STZ) solution: Dissolve STZ in 0.1mol / L sodium citrate buffer to prepare a STZ solution with a concentration of 10mg / mL, and filter it with a 0.22μm bacteria filter. Pay attention to the preparation in the dark, and use it now.

[0037] All mice were randomly divided into control group and model group, and fasted for 12 hours before operation. The mice in the model group were injected intraperitoneally with STZ solution at a dose of 70 mg / kg, and the control group was given...

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Abstract

The invention discloses a preparation method of an amnion-spongy chitosan composite double-layer wound dressing, and belongs to the technical field of medical dressings. The amnion-chitosan compositedouble-layer wound dressing is prepared by combining amnion dressing and spongy chitosan with excellent antibacterial and swelling properties by using a crosslinking method. The dressing has a wound healing rate of 87.67% on a 8th-day mice, and the wound tissue skin thickness of the mice reaches 693.9233 microns. In addition, the dressing increases the enrichment concentration of hemoglobin from 72.8 g/L of an amniotic membrane group to 685.39 g/L, increases the swelling coefficient to 800% from 300% of the amniotic membrane group, increases the tensile stress and nominal strain of the dressing to 1.14-2.29 MPa and 6.34-6.97%, achieves the deep wound filling effect, rapidly absorbs wound exudate at the same time, prevents wound infection, keeps the wet environment of the wound, and promotes wound healing.

Description

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Claims

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Application Information

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Owner JIANGNAN UNIV
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