Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of method for preparing casein calcium peptide

A casein calcium peptide and casein technology, applied in the field of bioactive peptides, can solve the problems of low purity, affecting shelf life, and low purity of CCP

Active Publication Date: 2022-07-08
GUANGZHOU MEDICAL UNIV +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, due to the poor specificity of the current CCP production methods, the quality of the isolated CCP is poor, such as low purity, large N / P molar ratio, bitter taste, heavy odor, easy to become viscous and poor stability when stored for a long time.
Due to the low purity of CCP and the large N / P molar ratio, it will affect its effect of preventing calcium phosphate precipitation in vivo or in vitro; CCP will easily become viscous after long-term storage, which will affect its shelf life; CCP’s bitter taste and heavy odor will affect its taste effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of method for preparing casein calcium peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 provides a method for preparing casein calcium peptide, comprising the following steps:

[0035] S1, get 10g of cow's milk casein, add water to it, stir and mix into cow's milk casein slurry, control the addition amount of water so that the substrate concentration of cow's milk casein is 15%;

[0036]S2, transfer the milk casein slurry in step S1 to a high pressure homogenizer, and homogenize at 85°C and 20MPa for 1.5h;

[0037] S3. Add compound enzyme A to the milk casein slurry of step S2, stir and mix, and said compound enzyme A is compounded by alkaline protease and trypsin in a weight ratio of 1.8:1. The addition amount is 2% of bovine milk casein, the pH is adjusted to 8.0, the reaction is placed at 50 °C for 1.5 h, and then placed at 90 °C for inactivation for 23 minutes after the reaction;

[0038] S4, transferring the enzymatic hydrolysis solution obtained after enzymatic hydrolysis in step S3 to a high-pressure homogenizer, and homogenizing at 55...

Embodiment 2

[0045] Embodiment 2 provides a method for preparing casein calcium peptide, comprising the following steps:

[0046] S1, get 10g cow's milk casein, add water to it, stir and mix into cow's milk casein slurry, control the addition amount of water so that the substrate concentration of cow's milk casein is 10%;

[0047] S2, transferring the milk casein slurry in step S1 to a high pressure homogenizer, and homogenizing at 80°C and 10MPa for 1h;

[0048] S3. Add compound enzyme A to the milk casein slurry of step S2, stir and mix, and said compound enzyme A is compounded by alkaline protease and trypsin in a weight ratio of 1.8:1. The addition amount is 1% of bovine milk casein, the pH is adjusted to 7.5, the reaction is placed at 45 °C for 2 hours, and then placed at 80 °C for inactivation for 30 minutes after the reaction;

[0049] S4, transferring the enzymatic hydrolysis solution obtained after enzymatic hydrolysis in step S3 to a high pressure homogenizer, and homogenizing a...

Embodiment 3

[0056] Embodiment 3 provides a method for preparing casein calcium peptide, comprising the following steps:

[0057] S1, get 10g of cow's milk casein, add water to it, stir and mix into cow's milk casein slurry, control the addition amount of water so that the substrate concentration of cow's milk casein is 20%;

[0058] S2, transferring the milk casein slurry in step S1 to a high pressure homogenizer, and homogenizing at 90°C and 30MPa for 2h;

[0059] S3. Add compound enzyme A to the milk casein slurry of step S2, stir and mix, and said compound enzyme A is compounded by alkaline protease and trypsin in a weight ratio of 1.8:1. The addition amount is 3% of bovine milk casein, the pH is adjusted to 8.5, the reaction is placed at 55 °C for 1 h, and then placed at 100 °C for 15 minutes of inactivation;

[0060] S4, transferring the enzymatic hydrolysis solution obtained after enzymatic hydrolysis in step S3 to a high-pressure homogenizer, and homogenizing at 60° C. and 8 MPa f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
working pressureaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of biologically active peptides, in particular to a method for preparing casein calcium peptides. The method combines homogenization treatment with compound enzyme A, compound enzyme B and compound enzyme C, and adopts a three-step enzymatic hydrolysis method to prepare CCP , the calculation result of CCP yield shows that the yield of CCP prepared by the method of the present invention is more than twice that of the conventional method; , the N / P molar ratio is smaller, the purity is higher, and the lactose content is greatly reduced; the measurement result of the bitterness value shows that, compared with the conventional method, the CCP prepared by the method of the invention has obvious debittering effect and good taste. It can be seen that the present invention not only improves the yield of casein calcium peptide, but also has low N / P molar ratio, less bitter taste, less stickiness, and greatly improved quality of the prepared casein calcium peptide.

Description

technical field [0001] The present invention relates to the technical field of biologically active peptides, in particular to a method for preparing casein calcium peptides. Background technique [0002] my country is one of the countries with a serious shortage of calcium intake, and finding an effective way to supplement calcium has become a major research area. Traditional calcium agents such as calcium gluconate, calcium lactate, etc., need to be added with vitamin D when taking them, and the calcium absorption rate is not high, and the effect of calcium supplementation is not good. In order to meet the body's need for calcium, in addition to eating calcium-rich foods, improving the absorption rate of calcium is the most effective calcium supplementation measure. [0003] Casein Calcium Pep-tide.CCP from bovine milk casein is a biologically active polypeptide containing phosphoserine residue, which contains about 12.5% ​​of casein calcium phosphate. CCP has the effect ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12P21/06C07K1/34
CPCC07K14/4732C12P21/06
Inventor 彭维刘飞
Owner GUANGZHOU MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com