Chromatograph and quantitative method for chromatographic analysis

Abstract

The invention provides a chromatograph and a quantitative method for chromatographic analysis. The chromatograph not only uses the calibration line based on the internal standard method, but also doesnot require the operation of adding a standard substance to an unknown sample, thereby simplifying the measurement and suppressing the decrease of the quantitative accuracy. The chromatograph includes a control unit and a storage unit. The control unit quantifies the predetermined measurement object. The storage unit stores calibration curve data based on the internal standard method, which is obtained by adding an internal standard of a predetermined concentration to the measurement object and has an intensity ratio between the intensity of the measurement object and the intensity of the internal standard. When the chromatograph measures the chromatogram of a QC sample containing an internal standard of a specified concentration and the chromatogram of an unknown sample containing a measurement target whose concentration is unknown but does not contain an internal standard, the control unit calculates the intensity ratio based on the intensity of the internal standard of the QC sample and the intensity of the measurement target of the unknown sample. The control unit then quantifies the measurement target of the unknown sample based on the calibration curve.

Description

technical field [0001] The invention relates to chromatographic instruments such as liquid chromatographs and gas chromatographs and a quantitative method for chromatographic analysis. Background technique [0002] Conventionally, an external standard method and an internal standard method are known as quantitative analysis in chromatography such as a liquid chromatograph (HPLC). Among them, the internal standard method prevents errors caused by daily changes in the sensitivity and state of the chromatograph, changes in sample injection volume, volatilization of solvents, etc., and its quantitative accuracy is higher than that of the external standard method. [0003] In addition, terms related to chromatographic analysis are specified in JIS K0214:2013. [0004] In this internal standard method, a sample containing a test substance with a known concentration and an (internal) standard substance at a certain concentration is measured in such a manner that the concentration ...

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Problems solved by technology

[0008] However, in the internal standard method, it is also necessary to add a certain concentration of standard substance to the sample when measuring an unknown sample, but there is such a problem: the operation of weighing a certain concentration of standard substance is complicated and requires proficiency, and if If the weighing of the standard substance is not correct, the quantitative accuracy will be reduced instead.

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