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35 results about "Quantitative methodology" patented technology
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Quantitative Research examines types of quantitative research and the use quantitative methodology in research papers. Quantitative research is a type of research methodology that involves a collection of numerical data to answer a specific research question.
Methods and systems for analyzing images of specimens processed by a programmable quantitative assay
ActiveUS20120163681A1Material analysis by optical meansCharacter and pattern recognitionTissue sampleBioinformatics
Disclosed are methods and systems for analyzing images of specimens processed by a programmable quantitative assay or more specifically a robust programmable quantitative dot assay, PDQA, that enable specimens to be imaged and assessed across a wide variety of conditions and applications. Specific embodiments directed to immunohistochemical applications provide more quantitative methods of imaging and assessing biological samples including tissue samples.
Owner:AGILENT TECH INC
Predictive Key Risk Indicator Identification Process Using Quantitative Methods
Methods, computer-readable media, and apparatuses are disclosed for identifying predictive key risk indicators (KRIs) for organizations and / or firms through the application of specific statistical and quantitative methods that are well integrated with qualitative adjustment. An indicator is a variable with the purpose of measuring change in a phenomena or process. A risk indicator is an indicator that estimates the potential for some form of resource degradation using mathematical formulas or models. Organization / enterprise key risk indicators are an essential arsenal in the risk management framework of any firm or organization and may be required by regulatory agencies.
Owner:BANK OF AMERICA CORP
Method and experiment system for simulating tunneling gas migration rule of coal mine
InactiveCN102733843AIncrease authenticityImprove reliabilityGas removalQualitative analysisGas explosion
The invention relates to a method and an experiment system for simulating a tunneling gas migration rule of a coal mine, aiming at providing a theory basis for safely and fast discharging gas. The invention discloses the method and the experiment system for simulating tunneling gas migration rule, firstly the tunneling experiment system is built, and then gas migration rules under turbulent jet action can be deeply explored by using orientated and quantitative methods under semi-closed condition, wherein the qualitative method is of non-contact measurement, qualitative analysis is conducted on the basis of not interfering flow field running characteristic, and the analysis result can be used as a basis of sensor arrangement in quantitative experiments. The quantitative method comprises the steps of arranging various sensors through multifunctional operation holes on a tunnel model, and exploring the tunneling gas migration rules through data analysis. The method and the experiment system can provide theory and data bases for fan control strategy under the normal ventilation and gas emission condition of tunnels, and also can provide guidance for preventing the gas explosion accidents in the tunnels.
Owner:BEIJING UNION UNIVERSITY
Method for quantifying microorganisms for producing lipopeptide surfactant in microbial flooding reservoir
ActiveCN102925563AHigh sensitivityImprove accuracyMicrobiological testing/measurementFluorescence/phosphorescenceMicrobial enhanced oil recoveryLinear relationship
The invention relates to a method for quantifying indigenous microorganisms for producing lipopeptide surfactant in microbial flooding reservoir. Primers are designed according to lipopeptide synthetase genes of microorganisms for producing lipopeptide surfactant, and fluorescent quantitative PCR (polymerase chain reaction) is performed. In optimized reaction procedures and reaction system, the lipopeptide synthetase genes obtained from a standard lipopeptide-producing strain is connected with a carrier to construct a recombinant plasmid used as a standard sample for real-time quantitative PCR; the standard sample is subjected to 10-time gradient dilution and used as a template for fluorescent quantitative PCR, and a standard curve is drawn; a microbial flooding reservoir sample is subjected to simple treatment and used as a template for fluorescent quantitative PCR; and whether data of replicate samples are identical and whether initial templates having different numbers of copies have the same amplification efficiency are judged according to linear relationship R2 and amplification efficiency E. When being used for detecting microorganisms for producing lipopeptide surfactant in reservoir under optimized reaction conditions, the lipopeptide synthetase specific primers have higher stability and sensitivity, thereby being widely used in the field of microbial enhanced oil recovery.
Owner:CHINA PETROLEUM & CHEM CORP +1
Quantitative method utilizing equivalent rock mass basic quality index to predict shield driving parameters
The invention relates to a quantitative method utilizing an equivalent rock mass basic quality index to predict shield driving parameters and relates to the geotechnical engineering and tunnel engineering investigation, design and construction technology field. According to the method, the equivalent rock mass basic quality index is taken as basis, geological segmentation of complex strata is carried out, and segmented statistics of the driving parameters is carried out; experience relationships among the driving rate, cutterhead torque and other driving parameters are calculated through gradual regression to acquire a general driving rate prediction model and a general cutterhead torque prediction model which adapt to uniform strata and the complex strata; through quantitative analysis on the corresponding relationships among a general driving rate prediction model coefficient, a general cutterhead torque prediction model coefficient and the equivalent rock mass basic quality index, the driving parameters are acquired through prediction. The method is advantaged in that the method has great theoretical meaning and engineering application values for construction scheme design, construction cost-construction period control and shield-surrounding rock interaction rule analysis.
Owner:NANJING UNIV OF TECH
Absolute quantitative analysis method of immunoglobulin G glycopeptide in serum
The invention provides an absolute quantitative analysis method of immunoglobulin G (IgG) glycopeptide in serum. The process includes the release of IgG glycopeptides in serum and the enrichment of IgG glycopeptides by chromatography. the collected IgG glycopeptide is divided into two parts, one part is subjected to profiling analysis of various glycoforms in the IgG glycopeptides, and another IgGglycopeptide is subjected to PNGase F enzymatic hydrolysis, and MRM quantitative analysis is performed on deglycosylated IgG peptides; and the content of glycopeptide corresponding to various glycoforms of IgG is calculated by combining the results of Profiling. The method of the invention can measure the molar concentration of each glycopeptide on each subtype of IgG in serum, and has the advantages of high sensitivity, good precision, high accuracy, high recovery rate, and good operability, and is suitable for high-flux absolute quantitative research of IgG glycopeptide in serum.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Fluorescence precise quantification method of integrity of DNA origami structure
ActiveCN107894411ADetermine completenessEasy to assess operationFluorescence/phosphorescenceDNA origamiFluorescence spectrometry
The invention relates to a fluorescence precise quantification method of the integrity of a DNA origami structure. The method includes the steps of synthesizing a DNA triangular origami structure andquantifying the change of the DNA triangular origami structure along with the change of ion concentration through a fluorescence spectrum. Specifically, an EvaGreen fluorescent dye serves as the quantification agent, and the integrity of the DNA triangular origami structure during environment changes is precisely quantified through fluorescence intensity. The invention belongs to the field of nanometer biological materials and biological detection. The method has the advantages of being precise, simple, simple in evaluating operation and capable of meeting the application requirements of the DNA nanometer origami structure in future.
Owner:SHANGHAI NAT ENG RES CENT FORNANOTECH
Operational Risk Back-Testing Process Using Quantitative Methods
Methods, computer-readable media, and apparatuses are disclosed for quantifying risk and control assessments. The risk includes both residual risk and direction of risk. Various aspects of the invention quantitatively compare the risk and control assessments against step-ahead losses using special regression models that are particularly applicable to this kind of data. The empirical comparison may be performed on both loss event frequency and severity in two different and separate dimensions. The empirical comparison may also be performed using losses extracted by even occurrence and event settlement dates in two separate dates.
Owner:BANK OF AMERICA CORP
Logging big data real time driving based in-service oil casing pipe defect automatic determination method
The invention discloses a Logging big data real time driving based in-service oil casing pipe defect automatic determination method. The method includes: determining the minimum wall thickness threshold and the maximum depth of an in-service oil casing pipe, the depth increment and the circumference increment recorded by a logging meter, and wall thickness big data of the oil casing pipe; performing a value compensation treatment and a smoothing treatment on the wall thickness big data of the oil casing pipe; determining a defect zone of the oil casing pipe; quantitatively calculating out basic parameters of the defect zone of the oil casing pipe; and determining the qualitative type of a defect of the oil casing pipe through a quantitative analysis method. The method can perform data treatment and analysis in real time according to the logging big data, can effectively detect the defect zone of the oil casing pipe, can quantitatively calculating out the basic parameters of the defect zone of the oil casing pipe so as to achieve accurate positioning of the defect zone, and can accurately determine the qualitative type of the defect of the oil casing pipe through the quantitative analysis method. The method is good in real-time performance, is high in accuracy, is high in reliability, is full in information, and is high in degree of automation.
Owner:BC P INC CHINA NAT PETROLEUM CORP +1
Method for performing accurate quantitative analysis on metal elements
ActiveCN110132916AReduce consumptionReduce system errorPreparing sample for investigationMaterial analysis by electric/magnetic meansQuantification methodsStandard methods
The invention relates to the field of analytical chemistry, in particular to a method for performing accurate quantitative analysis on metal elements by a patent technology combining a standard addition method and a differential method during accurate quantification. According to the analysis method, the standard addition method and the differential method are combined; a standard curve concentration calculation formula based on the differential method is designed; in combination with software calculation, a standard curve is established by using the standard addition method; the efficiency ofpreparing the standard curve is improved; and the interference caused by the matrix effect is effectively reduced, so that accurate quantification of the metal elements is realized. According to themethod, the calculated amount of preparing the standard curve by using the traditional standard addition method is greatly reduced, so that the quantitative analysis precision and speed of the harmfulmetal elements are improved; the standard addition method can replace the standard curve method (an external standard method); the accurate quantification method for the metal elements can be appliedto the detection of batch samples; and the method serves as a method for efficiently measuring the element concentration when a used instrument measures a special device without using the standard addition method.
Owner:李蒙
A DNA methylation degree quantifying method and application
InactiveCN109022556AAchieving absolute quantificationMeet the needs of biopsyMicrobiological testing/measurementDNA methylationModified dna
A DNA methylation degree quantifying method and application are provided. The method includes (1) adding sulfite modified DNA into a PCR system, and performing digital PCR; and (2) acquiring the DNA methylation degree according to the DNA methylation copy number and non-methylation copy number. The PCR system in the step (1) includes a Taqman probe, with the Taqman probe including a methylation Taqman probe or a non-methylation Taqman probe. The target gene modified with sulfite is detected with the methylation probe and the non-methylation probe separately, and the methylation copy number andthe non-methylation copy number of the gene to be detected are measured by the digital PCR accurately, thus achieving absolute quantification of the methylation degree of the target gene. The methodis suitable for detection of trace ctDNA in blood samples, is high in sensitivity, high in specificity, precise and rapid, and facilitates promotion in the technical field of liquid biopsy.
Owner:天津知因生物科技有限公司
Data analysis method for quantitative methods for b, y fragment ion pairs on second-level mass spectra from peptide fragments of same mass on first-level mass spectra
InactiveCN102809600AThe impact of correct certainty is reducedAdd optimization moduleMaterial analysis by electric/magnetic meansMass spectrometryQuantitative methodology
The invention relates to the technical field of proteomics, in particular to a data analysis method for quantitative methods for b, y fragment ion pairs on second-level mass spectra from peptide fragments of same mass on first-level mass spectra. The method is written by using a Matlab language and runs in a Matlab interface, and qualitative information of peptide fragments and protein is obtained by making ratio through extracting peak intensity information of b, y fragment ion pairs on tandem mass spectra and on the basis of a qualitative result of protein obtained by a SEQUEST search engine. According to the method, an optimization module for 'spectrogram resolution' is also added, so that the influence of a b, y fragment ion pair fragmentation spectrogram on the qualitative correctness of the peptide fragments is reduced. According to the method, a z statistical magnitude is also constructed to calculate the significance of quantitative protein, so that a reference evidence is provided for determining up-regulated and down-regulated changed protein in a practical sample. The invention provides a universal, open and accurate quantitative analysis means for development and application of novel quantitative proteomics methods for the b, y fragment ion pairs on second-level mass spectra from peptide fragments of same mass on first-level mass spectra.
Owner:FUDAN UNIV
Method for analysis metabolite difference between biological samples
Disclosed is a method of extracting all metabolites existing in a biological sample to detect a comprehensive difference between metabolites of a control group and a test group, thereby verifying significance thereof. More particularly, the invention comprises a first step of analyzing a fraction extracted with a solid phase extraction method, a fraction obtained by extracting the remnant fraction with a liquid-liquid extraction method and fractions extracted with the liquid-liquid extraction method at two pH different from each other after the hydrolysis, with a gas chromatography-mass spectrometry; a second step of converting the chromatogram result into numerical values capable of being statistically processed; and a third step of analyzing the numerical values with a principal component analysis (PCA) and a discriminant analysis (DA) to detect a difference between the control group and the test group. According to the invention, the difference between the two groups can be comprehensively detected with the areas of the peaks on the chromatogram, without a standard material or verified quantitative method. Therefore, it is possible to detect the metabolic change in the organism due to the disease or gene mutation, without an accurate quantitative analysis of the metabolites.
Owner:KOREA INST OF SCI & TECH
Quantitative method of atomic spectrometer
InactiveCN104713834AAvoid instabilityAvoid volatilityMaterial analysis using wave/particle radiationMaterial analysis by electric/magnetic meansInternal standardPhysical chemistry
The invention discloses a quantitative method of an atomic spectrometer. The quantitative method is characterized in that a standard addition method and an internal standard method are integrally combined. According to the quantitative method, in a quantitative test, all needed is to determine two or three data points, very accurate analysis determination results can be obtained. The quantitative method is suitable for determining samples under the conditions that the sample matrix interference is strong, and the determination conditions are unstable, and has the advantages of high speed, accurate results and the like.
Owner:TIANJIN NORMAL UNIVERSITY
Functional Platform for Rapid Capture and Removal of Nanoparticles
ActiveUS20180022604A1Facilitate rapid assessmentEasy to measureWater treatment parameter controlWater treatment compoundsBinding siteBiochemical engineering
Device, method, and system for nanoparticle capture, tracking, and / or detection. A functional paper-based platform is modified with capture ligands to create binding sites for nanoparticles. According to an embodiment, nanoparticle binding produces visual images of the particle content and distribution on the modified sensing surface, which provides capabilities for both NP sequestration and real-time detection. According to an embodiment the system may be utilized for environmental decontamination, fabrication of personal protective equipment, field monitoring, and epidemiological studies. The availability of inexpensive and easy-to-use quantitative methods can facilitate rapid assessment and measurement of NPs concentration and the level of exposure for large scale toxicological and epidemiological testing
Owner:CLARKSON UNIVERSITY
Virus titration method
InactiveCN101983244AQuantitatively accurateMicrobiological testing/measurementBiological material analysisContinuous evaluationBody fluid
Provided is a method for counting human herpes virus (HHV) harvested from body fluids and a kit for performing said method. In the past, skilled technique was required to accurately count HHV from body fluids. Provided is a novel titration method that allows simpler, and more accurate and efficient measurement of HHV counts in body fluids. Since the method can tolerate continuous evaluation of HHV counts in body fluids, it can also be used for quantitative evaluation of accumulated fatigue.
Owner:JAPAN TOBACCO INC
Intelligent qualitative and quantitative method for continuously monitoring gas chromatographic data on line
PendingCN113252828AProfessional Analysis ResultsIn-depth analysis of resultsComponent separationGas liquid chromatographicEngineering
The invention relates to the technical field of atmospheric monitoring, and discloses an intelligent qualitative and quantitative method for continuously monitoring gas chromatographic data on line, which comprises the following steps of: accessing data; performing regularization analysis; generating early warning information; and guiding production activities. The method is professional, and based on deep and comprehensive input data and a user-defined data analysis tool box, the analysis result of the system is deeper, more comprehensive and more professional; the expansibility and self-definition early warning rule engine can timely and effectively respond to the hard index requirement of the superior unit for the data production operation and maintenance work, and then problems can be timely found and solved; and the method is simple in structure, real-time and highly automatic in process, realizes a coordinated service of data production, analysis, auditing and early warning, and timely discovers known problems and generates early warning.
Owner:武汉市三藏科技有限责任公司
A quantitative method for steel samples combined with laser-induced breakdown spectroscopy
ActiveCN107632010BPlay an inhibitory roleReduce data dimensionalityAnalysis by thermal excitationCharacter and pattern recognitionData setLaser-induced breakdown spectroscopy
The invention discloses a method used for quantification of steel samples via combination with laser-induced breakdown spectroscopy. The method comprises following steps: 1, spectrum data is collectedso as to obtain two data sets; 2, the data sets are subjected to wavelet packet transformation, and spectrum peak intensity feature extraction so as to obtain wavelet packet energy, and the wavelet packet energy is adopted in a vector machine regression model as an input end; and 3, the vector machine regression model is established, and modeling prediction of content is carried out. According tothe method, wavelet packet transformation and an improved related vector machine kernel function quantitative analysis method are combined with laser-induced breakdown spectroscopy to realize rapid quantitative determination of steel samples.
Owner:段忆翔 +1
A kind of method that utilizes uplc-q-tof/ms technology to measure oligomeric guluronic acid rapidly
InactiveCN104897835BRealize accurate qualitative and quantitativeComponent separationTime-of-flight mass spectrometryMass Spectrometry-Mass Spectrometry
The invention discloses a method for rapidly determining oligomeric guluronic acid by using UPLC-Q-TOF / MS technology, which belongs to the technical field of ultra-high performance liquid chromatography-quadrupole tandem time-of-flight mass spectrometry UPLC-Q-TOF / MS detection. The invention quickly measures the oligoguluronic acid through the steps of preparation of standard products, sample pretreatment, chromatographic conditions, mass spectrometry conditions, establishment of a qualitative and quantitative method for the oligomeric guluronic acid, and the like. The present invention detects oligomeric guluronic acid quickly and accurately, and provides the characteristics of parent ions and fragment ions of oligomeric guluronic acid in mass spectrometry, thereby not only realizing the detection of guluronic acid monolayers in samples Accurate qualitative and quantitative analysis of sugars to tetrasaccharides. At the same time, the unknown oligomeric guluronic acid that may be contained in the sample can be speculated and analyzed based on the mass spectrometry information.
Owner:JIANGNAN UNIV
Chromatograph and quantitative method for chromatographic analysis
PendingCN110954637ASuppresses reduction in quantitative accuracyEasy to measureComponent separationSelective adsorptionInternal standardPhysical chemistry
The invention provides a chromatograph and a quantitative method for chromatographic analysis. The chromatograph not only uses the calibration line based on the internal standard method, but also doesnot require the operation of adding a standard substance to an unknown sample, thereby simplifying the measurement and suppressing the decrease of the quantitative accuracy. The chromatograph includes a control unit and a storage unit. The control unit quantifies the predetermined measurement object. The storage unit stores calibration curve data based on the internal standard method, which is obtained by adding an internal standard of a predetermined concentration to the measurement object and has an intensity ratio between the intensity of the measurement object and the intensity of the internal standard. When the chromatograph measures the chromatogram of a QC sample containing an internal standard of a specified concentration and the chromatogram of an unknown sample containing a measurement target whose concentration is unknown but does not contain an internal standard, the control unit calculates the intensity ratio based on the intensity of the internal standard of the QC sample and the intensity of the measurement target of the unknown sample. The control unit then quantifies the measurement target of the unknown sample based on the calibration curve.
Owner:HITACHI HIGH TECH SCI CORP
Automatic determination method of in-service tubing and casing defects based on real-time driving of well logging big data
ActiveCN105952434BImprove real-time performanceHigh precisionSurveyWell loggingQuantitative methodology
Owner:BC P INC CHINA NAT PETROLEUM CORP +1
Relative quantitative method of tubifex in sludge reduction technology and application of relative quantitative method
ActiveCN111676294AQuickly obtain the DNA copy number of the wormSolve the problem that it is difficult to complete the quantitative work of wormsMicrobiological testing/measurementBiological sludge treatmentTubifexSludge
The invention discloses a relative quantitative method of tubifex in sludge reduction technology and an application of the relative quantitative method. According to the relative quantitative method of tubifex, copy numbers of tubifex DNA in samples of different groups of objects to be tested can be quickly obtain by the simple operation of "sample mixing and drying + fluorescence quantitative PCR+ marking formula calculation", the relative amount of tubifex biomass in different groups of objects to be tested is judged by direct comparison of the copy numbers. The problem that quantitative work of the tubifex is difficult to finish by conventional methods such as counting and weighing at present is solved, and the objectives of comparative evaluation of sludge reduction effects of different sludge reduction reactors or judgment of the growth status of the tubifex in a single sludge reduction reactor are achieved. The method has broad application prospects.
Owner:SOUTH CHINA UNIV OF TECH
Application of microscopic linear array scanner for measuring rate of change of live organisms
The invention relates to application of microscopic linear array scanner for measuring the rate of change of live organisms, and to methods of sampling and quantitative measurements for microscopic scanner imaging in biological research. Due to technical and economic difficulties, most of the qualitative questions in the biological field can not be effectively addressed by using a valid sampling and quantitative measuring tool. Most researchers still collect data from fixed dead cells, and precious information of live cells during cultivation and daily observation are ignored. The invention creates a method of single lane scanning of live organisms by CCD linear array scanner to collect valid data while minimizing photonic interruption to said live organisms. The invention further creates a simple and manually-operated rotary mechanical system, through which a manual inverted microscope can be empowered for quantitative measuring the rate of change of live organisms.
Owner:斯蒂芬・立业・陈
A design method for landslide and rockfall protection based on 3D laser scanning
ActiveCN111639384BImprove efficiencyLow costGeometric CADDesign optimisation/simulationEnergy rateSoil science
The invention discloses a landslide and rockfall protection design method based on three-dimensional laser scanning, comprising the following steps: S1. collection of three-dimensional topographical data; S2. three-dimensional rockfall simulation without protection; S3. preliminarily planning the installation position of a passive protection system; S4. Three-dimensional rockfall simulation with protection; S5. Statistics Based on the results of step S4, count the number of rockfalls after interception under each working condition, that is, the interception rate of rockfalls IR, the energy rate of escaping rockfalls ERER and the cost of the protective net Cost: S6. Protection effect Quantitative analysis: determine a new quantitative method based on IR, ERER and Cost to evaluate the passive protection net; S7. Determine the protection scheme: select the appropriate installation position of the protection net according to the comprehensive level of the protection net and the actual engineering needs. The present invention selects the site of the optimal rockfall protection net based on the interception rate IR, the remaining rockfall energy rate ERER and the cost Cost, so as to evaluate the protection efficiency and cost of passive protection nets with different site selection positions, and screen out the optimal installation position to achieve low cost. Cost-effective, high-efficiency protection system design effect.
Owner:SICHUAN UNIV
Debugging quantitative method
PendingCN113917127AEliminate skill levelImprove debugging efficiencyPreparing sample for investigationBiological testingSimulationElectric machinery
The invention discloses a debugging quantitative method. The method comprises the following steps: S1, collecting test data of a principle machine for a quantitative volume V; s2, determining a function relational expression V = AN + B of the quantitative volume V and the quantitative pulse value N through linear fitting, wherein A and B represent constants; s3, setting instrument parameters; s4, determining a benchmark debugging pulse value N1 corresponding to the quantitative volume V by referring to the test data of the principle machine in the step S1; s5, controlling the stepping motor to complete sample adding according to the reference debugging pulse value N1; s6, weighing the weight of the debugging container after sample adding, and automatically calculating to obtain a debugging volume V1; s7, judging whether the debugging volume V1 meets the accuracy requirement of the quantitative volume V or not; if not, correcting the debugging pulse value N1, and repeatedly executing the steps S5 to S7; s8, debugging the volume V1 to meet the accuracy requirement of the quantitative volume V, and outputting a debugging pulse value N1 as a quantitative pulse value of the quantitative volume V in the instrument.
Owner:AUTOBIO LABTEC INSTR CO LTD
Quantitative method of microbial environmental change response force
PendingCN113322309AOvercoming the technical problem that the environmental response of species cannot be quantifiedMicrobiological testing/measurementMicroorganismIsotopic labeling
The invention relates to the technical field of environmental science and engineering, and discloses a quantitative method for environmental change response force of microorganisms. The method comprises the following steps: respectively setting soil samples with an isotope labeling group and an unlabeled control group corresponding to the isotope labeling group, the isotope labeling group containing at least one substrate corresponding to species elements, and the species elements in one of the substrates existing in an isotope form; obtaining the species performance of each species under different environmental conditions by using a stable isotope nucleic acid probe technology; and selecting a certain species as a target species A, setting a contrast environment condition a and a processing environment condition b, and calculating a standard effect value by utilizing the difference of species performances corresponding to the target species A under the two environment conditions, so as to measure the species environment response force of the target species A under the changing environment of the processing environment condition b. The method can realize quantitative analysis of microbial species function response force under environment change.
Owner:NANJING INST OF GEOGRAPHY & LIMNOLOGY
A Quantitative Method of Fish Diversity Based on Edna and Its Sampling and Filtering Device
ActiveCN109593829BAvoid cross contaminationIncrease concentrationBioreactor/fermenter combinationsBiological substance pretreatmentsZoologyQuantitative methodology
The invention discloses a quantitative method for fish diversity based on eDNA and a sampling and filtering device thereof, and belongs to the field of fish diversity research. According to the method, sample DNA extraction is performed by adopting a filtering method, the eDNA-based quantitative sampling and filtering device for fish diversity is adopted, and stirring is performed at the same timeof multiple suction filtration, so that an experimental period is greatly shortened, and meanwhile, samples can be effectively avoided from being cross-contaminated in the experimental stage; the filtered samples are used for DNA extraction within 24 hours, which can effectively reduce DNA degradation; by adopting human tissue and a blood extract as an extractant, the maximum extraction amount can be obtained, and the extracted DNA concentration and purity are relatively high; by adopting DNA clone sequencing, the experiment cost can be reduced; an absolute quantitative PCR method is used forquantitative research of fish, absolute quantification can be used for determining the copy number or concentration of genes in the samples by a standard curve and a dissolution curve, the specificity is high and the accuracy is good.
Owner:BEIJING NORMAL UNIVERSITY
A method for precise quantitative analysis of metal elements
ActiveCN110132916BReduce consumptionReduce system errorPreparing sample for investigationMaterial analysis by electric/magnetic meansChemical physicsBulk samples
The invention relates to the field of analytical chemistry, and more specifically relates to a method for precise quantitative analysis of metal elements using a patented technology combining a standard addition method and a differential method. This analysis method adopts the combination of standard addition method and difference method, and designs the standard curve concentration calculation formula based on the difference method. Combined with software calculation, the standard addition method is used to establish the standard curve, which improves the efficiency of preparing the standard curve and effectively reduces the matrix concentration. The interference caused by the effect, so as to realize the precise quantification of metal elements. This method greatly reduces the amount of calculation when using the traditional standard addition method to prepare a standard curve, thereby improving the accuracy and speed of quantitative analysis of harmful metal elements, so that the standard addition method can replace the standard curve method (external standard method), allowing metal elements to be accurately Quantitative methods can be applied to the detection of batch samples and as an efficient method of measuring element concentrations when the instrument used does not have a special device for measurement using the standard addition method.
Owner:李蒙
A method for protein quantification based on the dimethylation-tagged DIA strategy
ActiveCN109142737BImprove Quantitative AccuracyGood reproducibilityBiological testingQuantitative accuracyFLAG peptide
The present invention relates to a label-based DIA quantification method. It utilizes dimethylation reaction to label peptides, realizes multiple labeling of peptide samples through the organic combination of various isotope forms of dimethylation labeling reagents, uses data-independent (DIA) acquisition mode to collect data, and extracts the corresponding Extracted ion chromatograms of multiple parent ions and product ions in the DIA data, using the extracted peak areas for multiple quantitative analysis of proteins. The advantages of this method are: high quantitative accuracy, good reproducibility, high labeling efficiency, simple method, low requirements on the reproducibility of the liquid-mass system, easy multi-dimensional separation of samples, and high throughput of quantitative proteome analysis. The invention improves the quantitative accuracy and throughput of the DIA strategy by marking, and has obvious advantages in the analysis of large sample proteomes.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Multi-peak quantification method based on photoionization ion mobility spectrometry
InactiveCN112649494AReduce analysis timeSimplify Quantitative OperationsMaterial analysis by electric/magnetic meansParticle physicsIon-mobility spectrometry
The invention discloses a multi-peak quantification method based on photoionization ion mobility spectrometry. The method comprises the following steps: analyzing the maximum signal intensity of reagent molecules and the integral area of a certain product ion peak in the whole analysis process of a substance which generates two product ion peaks and has a fixed ion number ratio in an ion-molecular reaction in the ion mobility spectrometry of a negative ion mode; and establishing a linear quantitative relationship between the ratio of the integral area to the maximum signal intensity and the concentration of the substance to be detected in the sample. Propofol in blood is taken as an example, a standard quantitative curve of the photoionization ion mobility spectrometry is established in a concentration range of 0.1-20 ngmuL<-1 >, wherein Y= 0.05 X-0.0004 and a correlation coefficient R<2>= 0.997. The method enlarges the application range of the ion mobility spectrometry as a quantitative instrument, shortens data analysis time, simplifies quantitative operation, is quicker and more accurate, and has a wider application prospect.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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