34 results about "Quantitative methodology" patented technology

The invention relates to a fluorescence precise quantification method of the integrity of a DNA origami structure. The method includes the steps of synthesizing a DNA triangular origami structure andquantifying the change of the DNA triangular origami structure along with the change of ion concentration through a fluorescence spectrum. Specifically, an EvaGreen fluorescent dye serves as the quantification agent, and the integrity of the DNA triangular origami structure during environment changes is precisely quantified through fluorescence intensity. The invention belongs to the field of nanometer biological materials and biological detection. The method has the advantages of being precise, simple, simple in evaluating operation and capable of meeting the application requirements of the DNA nanometer origami structure in future.

The invention relates to the field of analytical chemistry, in particular to a method for performing accurate quantitative analysis on metal elements by a patent technology combining a standard addition method and a differential method during accurate quantification. According to the analysis method, the standard addition method and the differential method are combined; a standard curve concentration calculation formula based on the differential method is designed; in combination with software calculation, a standard curve is established by using the standard addition method; the efficiency ofpreparing the standard curve is improved; and the interference caused by the matrix effect is effectively reduced, so that accurate quantification of the metal elements is realized. According to themethod, the calculated amount of preparing the standard curve by using the traditional standard addition method is greatly reduced, so that the quantitative analysis precision and speed of the harmfulmetal elements are improved; the standard addition method can replace the standard curve method (an external standard method); the accurate quantification method for the metal elements can be appliedto the detection of batch samples; and the method serves as a method for efficiently measuring the element concentration when a used instrument measures a special device without using the standard addition method.

A DNA methylation degree quantifying method and application are provided. The method includes (1) adding sulfite modified DNA into a PCR system, and performing digital PCR; and (2) acquiring the DNA methylation degree according to the DNA methylation copy number and non-methylation copy number. The PCR system in the step (1) includes a Taqman probe, with the Taqman probe including a methylation Taqman probe or a non-methylation Taqman probe. The target gene modified with sulfite is detected with the methylation probe and the non-methylation probe separately, and the methylation copy number andthe non-methylation copy number of the gene to be detected are measured by the digital PCR accurately, thus achieving absolute quantification of the methylation degree of the target gene. The methodis suitable for detection of trace ctDNA in blood samples, is high in sensitivity, high in specificity, precise and rapid, and facilitates promotion in the technical field of liquid biopsy.

Disclosed is a method of extracting all metabolites existing in a biological sample to detect a comprehensive difference between metabolites of a control group and a test group, thereby verifying significance thereof. More particularly, the invention comprises a first step of analyzing a fraction extracted with a solid phase extraction method, a fraction obtained by extracting the remnant fraction with a liquid-liquid extraction method and fractions extracted with the liquid-liquid extraction method at two pH different from each other after the hydrolysis, with a gas chromatography-mass spectrometry; a second step of converting the chromatogram result into numerical values capable of being statistically processed; and a third step of analyzing the numerical values with a principal component analysis (PCA) and a discriminant analysis (DA) to detect a difference between the control group and the test group. According to the invention, the difference between the two groups can be comprehensively detected with the areas of the peaks on the chromatogram, without a standard material or verified quantitative method. Therefore, it is possible to detect the metabolic change in the organism due to the disease or gene mutation, without an accurate quantitative analysis of the metabolites.

Provided is a method for counting human herpes virus (HHV) harvested from body fluids and a kit for performing said method. In the past, skilled technique was required to accurately count HHV from body fluids. Provided is a novel titration method that allows simpler, and more accurate and efficient measurement of HHV counts in body fluids. Since the method can tolerate continuous evaluation of HHV counts in body fluids, it can also be used for quantitative evaluation of accumulated fatigue.

The invention discloses a method used for quantification of steel samples via combination with laser-induced breakdown spectroscopy. The method comprises following steps: 1, spectrum data is collectedso as to obtain two data sets; 2, the data sets are subjected to wavelet packet transformation, and spectrum peak intensity feature extraction so as to obtain wavelet packet energy, and the wavelet packet energy is adopted in a vector machine regression model as an input end; and 3, the vector machine regression model is established, and modeling prediction of content is carried out. According tothe method, wavelet packet transformation and an improved related vector machine kernel function quantitative analysis method are combined with laser-induced breakdown spectroscopy to realize rapid quantitative determination of steel samples.

The invention discloses a method for rapidly determining oligomeric guluronic acid by using UPLC-Q-TOF / MS technology, which belongs to the technical field of ultra-high performance liquid chromatography-quadrupole tandem time-of-flight mass spectrometry UPLC-Q-TOF / MS detection. The invention quickly measures the oligoguluronic acid through the steps of preparation of standard products, sample pretreatment, chromatographic conditions, mass spectrometry conditions, establishment of a qualitative and quantitative method for the oligomeric guluronic acid, and the like. The present invention detects oligomeric guluronic acid quickly and accurately, and provides the characteristics of parent ions and fragment ions of oligomeric guluronic acid in mass spectrometry, thereby not only realizing the detection of guluronic acid monolayers in samples Accurate qualitative and quantitative analysis of sugars to tetrasaccharides. At the same time, the unknown oligomeric guluronic acid that may be contained in the sample can be speculated and analyzed based on the mass spectrometry information.

The invention discloses a Logging big data real time driving based in-service oil casing pipe defect automatic determination method. The method includes: determining the minimum wall thickness threshold and the maximum depth of an in-service oil casing pipe, the depth increment and the circumference increment recorded by a logging meter, and wall thickness big data of the oil casing pipe; performing a value compensation treatment and a smoothing treatment on the wall thickness big data of the oil casing pipe; determining a defect zone of the oil casing pipe; quantitatively calculating out basic parameters of the defect zone of the oil casing pipe; and determining the qualitative type of a defect of the oil casing pipe through a quantitative analysis method. The method can perform data treatment and analysis in real time according to the logging big data, can effectively detect the defect zone of the oil casing pipe, can quantitatively calculating out the basic parameters of the defect zone of the oil casing pipe so as to achieve accurate positioning of the defect zone, and can accurately determine the qualitative type of the defect of the oil casing pipe through the quantitative analysis method. The method is good in real-time performance, is high in accuracy, is high in reliability, is full in information, and is high in degree of automation.

The invention relates to application of microscopic linear array scanner for measuring the rate of change of live organisms, and to methods of sampling and quantitative measurements for microscopic scanner imaging in biological research. Due to technical and economic difficulties, most of the qualitative questions in the biological field can not be effectively addressed by using a valid sampling and quantitative measuring tool. Most researchers still collect data from fixed dead cells, and precious information of live cells during cultivation and daily observation are ignored. The invention creates a method of single lane scanning of live organisms by CCD linear array scanner to collect valid data while minimizing photonic interruption to said live organisms. The invention further creates a simple and manually-operated rotary mechanical system, through which a manual inverted microscope can be empowered for quantitative measuring the rate of change of live organisms.

The invention discloses a landslide and rockfall protection design method based on three-dimensional laser scanning, comprising the following steps: S1. collection of three-dimensional topographical data; S2. three-dimensional rockfall simulation without protection; S3. preliminarily planning the installation position of a passive protection system; S4. Three-dimensional rockfall simulation with protection; S5. Statistics Based on the results of step S4, count the number of rockfalls after interception under each working condition, that is, the interception rate of rockfalls IR, the energy rate of escaping rockfalls ERER and the cost of the protective net Cost: S6. Protection effect Quantitative analysis: determine a new quantitative method based on IR, ERER and Cost to evaluate the passive protection net; S7. Determine the protection scheme: select the appropriate installation position of the protection net according to the comprehensive level of the protection net and the actual engineering needs. The present invention selects the site of the optimal rockfall protection net based on the interception rate IR, the remaining rockfall energy rate ERER and the cost Cost, so as to evaluate the protection efficiency and cost of passive protection nets with different site selection positions, and screen out the optimal installation position to achieve low cost. Cost-effective, high-efficiency protection system design effect.

The invention discloses a debugging quantitative method. The method comprises the following steps: S1, collecting test data of a principle machine for a quantitative volume V; s2, determining a function relational expression V = AN + B of the quantitative volume V and the quantitative pulse value N through linear fitting, wherein A and B represent constants; s3, setting instrument parameters; s4, determining a benchmark debugging pulse value N1 corresponding to the quantitative volume V by referring to the test data of the principle machine in the step S1; s5, controlling the stepping motor to complete sample adding according to the reference debugging pulse value N1; s6, weighing the weight of the debugging container after sample adding, and automatically calculating to obtain a debugging volume V1; s7, judging whether the debugging volume V1 meets the accuracy requirement of the quantitative volume V or not; if not, correcting the debugging pulse value N1, and repeatedly executing the steps S5 to S7; s8, debugging the volume V1 to meet the accuracy requirement of the quantitative volume V, and outputting a debugging pulse value N1 as a quantitative pulse value of the quantitative volume V in the instrument.

The invention discloses a quantitative method for fish diversity based on eDNA and a sampling and filtering device thereof, and belongs to the field of fish diversity research. According to the method, sample DNA extraction is performed by adopting a filtering method, the eDNA-based quantitative sampling and filtering device for fish diversity is adopted, and stirring is performed at the same timeof multiple suction filtration, so that an experimental period is greatly shortened, and meanwhile, samples can be effectively avoided from being cross-contaminated in the experimental stage; the filtered samples are used for DNA extraction within 24 hours, which can effectively reduce DNA degradation; by adopting human tissue and a blood extract as an extractant, the maximum extraction amount can be obtained, and the extracted DNA concentration and purity are relatively high; by adopting DNA clone sequencing, the experiment cost can be reduced; an absolute quantitative PCR method is used forquantitative research of fish, absolute quantification can be used for determining the copy number or concentration of genes in the samples by a standard curve and a dissolution curve, the specificity is high and the accuracy is good.

The invention relates to the field of analytical chemistry, and more specifically relates to a method for precise quantitative analysis of metal elements using a patented technology combining a standard addition method and a differential method. This analysis method adopts the combination of standard addition method and difference method, and designs the standard curve concentration calculation formula based on the difference method. Combined with software calculation, the standard addition method is used to establish the standard curve, which improves the efficiency of preparing the standard curve and effectively reduces the matrix concentration. The interference caused by the effect, so as to realize the precise quantification of metal elements. This method greatly reduces the amount of calculation when using the traditional standard addition method to prepare a standard curve, thereby improving the accuracy and speed of quantitative analysis of harmful metal elements, so that the standard addition method can replace the standard curve method (external standard method), allowing metal elements to be accurately Quantitative methods can be applied to the detection of batch samples and as an efficient method of measuring element concentrations when the instrument used does not have a special device for measurement using the standard addition method.

The present invention relates to a label-based DIA quantification method. It utilizes dimethylation reaction to label peptides, realizes multiple labeling of peptide samples through the organic combination of various isotope forms of dimethylation labeling reagents, uses data-independent (DIA) acquisition mode to collect data, and extracts the corresponding Extracted ion chromatograms of multiple parent ions and product ions in the DIA data, using the extracted peak areas for multiple quantitative analysis of proteins. The advantages of this method are: high quantitative accuracy, good reproducibility, high labeling efficiency, simple method, low requirements on the reproducibility of the liquid-mass system, easy multi-dimensional separation of samples, and high throughput of quantitative proteome analysis. The invention improves the quantitative accuracy and throughput of the DIA strategy by marking, and has obvious advantages in the analysis of large sample proteomes.

The invention discloses a multi-peak quantification method based on photoionization ion mobility spectrometry. The method comprises the following steps: analyzing the maximum signal intensity of reagent molecules and the integral area of a certain product ion peak in the whole analysis process of a substance which generates two product ion peaks and has a fixed ion number ratio in an ion-molecular reaction in the ion mobility spectrometry of a negative ion mode; and establishing a linear quantitative relationship between the ratio of the integral area to the maximum signal intensity and the concentration of the substance to be detected in the sample. Propofol in blood is taken as an example, a standard quantitative curve of the photoionization ion mobility spectrometry is established in a concentration range of 0.1-20 ngmuL<-1 >, wherein Y= 0.05 X-0.0004 and a correlation coefficient R<2>= 0.997. The method enlarges the application range of the ion mobility spectrometry as a quantitative instrument, shortens data analysis time, simplifies quantitative operation, is quicker and more accurate, and has a wider application prospect.

Device, method, and system for nanoparticle capture, tracking, and / or detection. A functional paper-based platform is modified with capture ligands to create binding sites for nanoparticles. According to an embodiment, nanoparticle binding produces visual images of the particle content and distribution on the modified sensing surface, which provides capabilities for both NP sequestration and real-time detection. According to an embodiment the system may be utilized for environmental decontamination, fabrication of personal protective equipment, field monitoring, and epidemiological studies. The availability of inexpensive and easy-to-use quantitative methods can facilitate rapid assessment and measurement of NPs concentration and the level of exposure for large scale toxicological and epidemiological testing.