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Multi-peak quantification method based on photoionization ion mobility spectrometry

A technology of ion mobility spectrometry and ionization, applied in the field of multi-peak quantification based on photoionization ion mobility spectrometry, can solve problems such as inapplicability, limitation of ion mobility spectrometry application scenarios, complex ionization reactions, etc., to expand the scope of application and shorten the data The effect of long analysis time and broad application prospects

Inactive Publication Date: 2021-04-13
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, on the one hand, the above methods use radioactive ionization sources, which have certain limitations on the application scenarios of ion mobility spectrometry; Substances with complex reactions and multiple product ion peaks are not suitable

Method used

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  • Multi-peak quantification method based on photoionization ion mobility spectrometry
  • Multi-peak quantification method based on photoionization ion mobility spectrometry
  • Multi-peak quantification method based on photoionization ion mobility spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] According to the above conditions, blank blood was injected and determined by ion mobility spectrometry. figure 1 is the reagent ion O 2 - The tracking trend line of the peak thermal analysis signal can easily get the O during a single analysis. 2 - Maximum signal strength (I m ) and the integral value within 0~60s.

Embodiment 2

[0037] According to the above conditions, the concentration of propofol was 5 ng μL -1 The blood samples were injected and determined by ion mobility spectrometry. figure 2 Propofol product ion M·O 2 - tracking trend line. Integrate the curve within the range of 0-60s, and subtract the integral value corresponding to the blank blood injection from the obtained integral value to obtain a concentration of 5 ngμL -1 The corresponding integral area of ​​the sample.

Embodiment 3

[0039] According to the above conditions, take the prepared 1, 2.5, 5, 7.5, 10, 12.5ngμL -1 of blood samples, determined by ion mobility spectrometry. The signals of the two product ion peaks of propofol were tracked simultaneously, and the integrated areas of the two peaks at each concentration were calculated separately. The integral area at each concentration is compared, and the ratio is plotted against the concentration of propofol, as image 3 shown. It can be seen that the integrated areas of the two product ion peaks of propofol do not fluctuate with the concentration, that is, propofol will undergo ion-molecule reactions R3 and R4 in the ion mobility spectrum of negative ion mode, and the generated [M-H] - and M·O 2 - The ratio of the number of ions is fixed.

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Abstract

The invention discloses a multi-peak quantification method based on photoionization ion mobility spectrometry. The method comprises the following steps: analyzing the maximum signal intensity of reagent molecules and the integral area of a certain product ion peak in the whole analysis process of a substance which generates two product ion peaks and has a fixed ion number ratio in an ion-molecular reaction in the ion mobility spectrometry of a negative ion mode; and establishing a linear quantitative relationship between the ratio of the integral area to the maximum signal intensity and the concentration of the substance to be detected in the sample. Propofol in blood is taken as an example, a standard quantitative curve of the photoionization ion mobility spectrometry is established in a concentration range of 0.1-20 ngmuL<-1 >, wherein Y= 0.05 X-0.0004 and a correlation coefficient R<2>= 0.997. The method enlarges the application range of the ion mobility spectrometry as a quantitative instrument, shortens data analysis time, simplifies quantitative operation, is quicker and more accurate, and has a wider application prospect.

Description

technical field [0001] The invention relates to the technical field of ion mobility spectrometry application and analysis and detection, in particular to a multi-peak quantitative method based on photoionization ion mobility spectrometry. Background technique [0002] Ion mobility spectrometry is a separation and detection technology based on the different moving speeds of gas phase ions in an external electric field under atmospheric pressure. It has the advantages of fast detection speed, high sensitivity, and low price. It is a real-time dynamic detection technology with great application prospects. . At present, ion mobility spectrometry technology is widely used in public security, environmental monitoring, clinical diagnosis and other fields. However, due to the influence of various external conditions and the conditions of the instrument itself, the quantitative accuracy of ion mobility spectrometry is limited. The ionization source provides energy to ionize the reag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/622
CPCG01N27/622
Inventor 李海洋肖瑶王新
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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