Method for establishing UPLC fingerprint spectrum of anti-hysteritis soft capsule

A technique for fingerprinting and establishing methods, which is applied in the field of Kanggongyan soft capsules, can solve the problems that the fingerprints and detection methods of Kanggongyan soft capsules have not been reported, and achieve good stability, high precision and good repeatability Effect

Pending Publication Date: 2021-06-29
GUIZHOU HUIZHENG PHARMA CO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, only the HPLC fingerprints of Kanggongyan series preparations (tablets, granules, and capsules) have been studied, and the fingerprints and detection methods of Kanggongyan Soft Capsules have not been reported yet.

Method used

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  • Method for establishing UPLC fingerprint spectrum of anti-hysteritis soft capsule
  • Method for establishing UPLC fingerprint spectrum of anti-hysteritis soft capsule
  • Method for establishing UPLC fingerprint spectrum of anti-hysteritis soft capsule

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Embodiment Construction

[0064] The present invention will be further described below in conjunction with the examples, but not as a basis for limiting the present invention.

[0065] Example. A method for establishing the UPLC fingerprint of anti-gongyan soft capsules, the establishment steps are:

[0066] (1) Preparation of mixed reference solution: Accurately weigh appropriate amounts of norisoboldine, leonurine hydrochloride, forsythoside B, verbascoside, chrysoside, and isorrascoside, put them in a volumetric flask, add 50% Diluted with methanol and shaken well, the obtained concentrations were 0.05 (norisoboldine), 0.15 (leonurine hydrochloride), 0.15 (forsythoside B), 0.15 (verbasoside), 0.15 (chryposide), 0.15(isoverbasin) mg·mL -1 mixed reference solution.

[0067] (2) Preparation of test solution: take 1.0g of anti-gongyan soft capsule content, accurately weigh it, place it in a round-bottomed flask, accurately add 50 mL of 50% methanol, weigh it, reflux for 1 hour, let it cool, and weigh...

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Abstract

The invention discloses a method for establishing an anti-hysteritis soft capsule UPLC fingerprint spectrum. The method comprises the following steps: (1) preparing a mixed reference substance solution; (2) preparing a test solution; (3) manufacturing a fingerprint spectrum; and (4) confirming a standard fingerprint spectrum: according to the method provided by the invention, establishing the UPLC fingerprint spectrum of the anti-hysteritis soft capsule, determining 12 common peaks through analysis and comparison, and forming fingerprint characteristics of the anti-hysteritis soft capsule by the common peaks as the UPLC fingerprint spectrum of the anti-hysteritis soft capsule. The method provided by the invention has the advantages of rapidness, convenience, high precision, good stability and good repeatability, and can be used for systematically and comprehensively evaluating the quality of the anti-hysteritis soft capsule. According to the method disclosed by the invention, the peaks of norisoboldine, leonurine hydrochloride, forsythiaside B, verbascoside, poliumoside and isoacteoside can be directly found by taking the fifth peak forsythiaside B as a reference peak.

Description

technical field [0001] The invention relates to the technical field of anti-gongyan soft capsules, in particular to a method for establishing a UPLC fingerprint of anti-gongyan soft capsules. Background technique [0002] Kanggongyan Soft Capsule is nine new types of traditional Chinese medicines derived from Kanggongyan Tablets. It consists of Guangdong Zizhu Dry Extract, Motherwort Dry Extract and Wuyao Dry Extract. The effect of blood stasis, heat-clearing and detoxification; Motherwort has the effect of promoting blood circulation and regulating menstruation, diuresis and swelling, clearing heat and detoxifying; black medicine has the effect of promoting qi and relieving pain, warming kidney and dispelling cold. Anti-metritis series preparations are effective in clearing heat, dispelling dampness, removing blood stasis and stopping vaginal discharge. They are mainly used for vaginal discharge caused by damp-heat injection, such as red vaginal discharge, excessive odor an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8631G01N30/8686
Inventor 高秀丽张敏王鹏娇罗伟张硕袁敏艳
Owner GUIZHOU HUIZHENG PHARMA CO
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