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Quantitative method of microbial environmental change response force

A technology of environmental change and responsiveness, applied in the field of environmental science and engineering, capable of solving quantitative analysis and other problems

Pending Publication Date: 2021-08-31
NANJING INST OF GEOGRAPHY & LIMNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, under different spatial and temporal conditions, there are no relevant research reports on how the performance of microbial species responds to environmental changes, including the intensity and direction of response force, how to quantitatively analyze these response forces, etc.

Method used

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  • Quantitative method of microbial environmental change response force
  • Quantitative method of microbial environmental change response force
  • Quantitative method of microbial environmental change response force

Examples

Experimental program
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Effect test

Embodiment 1

[0072] Taking the soil samples of four altitude gradients (1750m, 2000m, 2250m and 2500m) as an example, a total of 12 samples (including 3 repetitions) were collected from low to high altitudes for subsequent labeling experiments, and the specific implementation steps of the labeling experiments for each sample for:

[0073] Step 1, by adding labeled substrate ( 13 C-glucose and 18 O-H 2 O) and conventional substrates ( 12 C-glucose, H 2 16 O, NH 4 NO 3 ) to carry out the soil labeling experiment, eight treatment groups are set altogether, specifically: H 2 18 O marker group (18O;b), 13 C-glucose+H 2 16 O marker group (13C; g), 12 C-glucose+H 2 18 O flag group (12C_18O; d), 13 C-glucose+H 2 16 O+NH 4 NO 3 marker set (13C_N; h), 12 C-glucose+H 2 18 O+NH 4 NO 3 flagset(12C_18O_N; f); H 2 16 O control group (16O; a), 12 C-glucose+H 2 16 O control group (12C; c), 12 C-glucose+H 2 16 O+NH 4 NO 3 Control group (12C_N; e), see figure 1 ;

[0074] ...

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Abstract

The invention relates to the technical field of environmental science and engineering, and discloses a quantitative method for environmental change response force of microorganisms. The method comprises the following steps: respectively setting soil samples with an isotope labeling group and an unlabeled control group corresponding to the isotope labeling group, the isotope labeling group containing at least one substrate corresponding to species elements, and the species elements in one of the substrates existing in an isotope form; obtaining the species performance of each species under different environmental conditions by using a stable isotope nucleic acid probe technology; and selecting a certain species as a target species A, setting a contrast environment condition a and a processing environment condition b, and calculating a standard effect value by utilizing the difference of species performances corresponding to the target species A under the two environment conditions, so as to measure the species environment response force of the target species A under the changing environment of the processing environment condition b. The method can realize quantitative analysis of microbial species function response force under environment change.

Description

technical field [0001] The invention relates to the field of environmental science and engineering technology, in particular to a quantitative method for the response force of microbial environmental changes. Background technique [0002] The physiological and metabolic functions of specific microorganisms are the result of long-term interactions with other organisms and the natural environment. Studying the molecular regulation mechanism of important physiological processes of microorganisms in complex environments at the community level can provide a more accurate understanding of the mechanism, driving force and possible evolutionary direction of microbial diversity formation. Compared with the pure culture system, the number and variety of microorganisms in the natural environment are large, and most species lack pure culture, which greatly hinders people from obtaining the physiological characteristics of the species, so it is impossible to know the functional role of t...

Claims

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Application Information

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IPC IPC(8): C12Q1/6851C12Q1/06C12Q1/04
CPCC12Q1/6851C12Q2531/113C12Q2563/107C12Q2535/122
Inventor 胡盎王建军
Owner NANJING INST OF GEOGRAPHY & LIMNOLOGY
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