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Application of microscopic linear array scanner for measuring rate of change of live organisms

A biological, automatic scanning technology, applied in microscopes, instruments, measuring devices, etc., can solve problems such as technical difficulties

Inactive Publication Date: 2011-08-17
斯蒂芬・立业・陈
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to technical difficulties, the vast majority of scientific research quantitative questions cannot be answered with effective quantitative tools.

Method used

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  • Application of microscopic linear array scanner for measuring rate of change of live organisms
  • Application of microscopic linear array scanner for measuring rate of change of live organisms
  • Application of microscopic linear array scanner for measuring rate of change of live organisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0047] Embodiment 1: Determining the rate of change of living organisms with a fully automatic microscanner.

[0048] Computer-programmed microscopic linear scanning is an expensive system. Its XYZ micron-level precise three-dimensional movement and instant auto-focus are controlled by special computer software. Micro-platform culture devices for 96-well plates are available from a number of suppliers, such as Warner Instruments, Inc., USA.

[0049] Based on the above-mentioned device, the present invention can perform secondary single-line scanning sampling on a part of living organisms, and the steps are as follows:

[0050] 1. Select an appropriate resolution and preset the above-mentioned computer program to perform non-overlapping single-line scanning sampling on a 96-well plate along a series of XY coordinates in order to reduce the light interference of the scanning on the above-mentioned living organisms.

[0051] 2. Position the 96-well culture plate in the platform...

Embodiment approach 2

[0058] Embodiment 2: Determining the rate of change of living organisms manually

[0059] After decades of long-term accumulation, thousands of manual inverted microscopes have been put into use worldwide. For everyday observation purposes, these low-end manual microscopes are more popular than motor-driven microscopes. They are attractive for their quick and free manual adjustments, but the problem is that they do not record the XY coordinates that the platform passes through. Therefore, it is impossible to perform secondary shooting on the same group of target groups in sequence. Unfortunately, the habit of low-end manual type work led to a loss of creativity. Indeed, the routine cellular observation process is a valuable opportunity to collect data from living cells.

[0060] The present invention uses comprehensive knowledge from instrumentation engineering and mechanical engineering to create a simple rotating mechanical system that can be combined with a low-speed man...

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Abstract

The invention relates to application of microscopic linear array scanner for measuring the rate of change of live organisms, and to methods of sampling and quantitative measurements for microscopic scanner imaging in biological research. Due to technical and economic difficulties, most of the qualitative questions in the biological field can not be effectively addressed by using a valid sampling and quantitative measuring tool. Most researchers still collect data from fixed dead cells, and precious information of live cells during cultivation and daily observation are ignored. The invention creates a method of single lane scanning of live organisms by CCD linear array scanner to collect valid data while minimizing photonic interruption to said live organisms. The invention further creates a simple and manually-operated rotary mechanical system, through which a manual inverted microscope can be empowered for quantitative measuring the rate of change of live organisms.

Description

technical field [0001] The invention relates to the application of a micro scanner in biology. More specifically, the present invention relates to a method of using a microscanner to measure the rate of change of a living organism while minimizing optical interference to the living organism during the scanning process. Background technique [0002] CCD array sensors are commonly used electronic components. It comes in two types: linear arrays and area arrays. Area array sensors are mostly used in digital cameras, while linear array sensors are used in scanning machines. At present, the research and development of linear array sensors in biological applications lags far behind the application level of area array sensors. [0003] For example, Aperio (Vista, CA, US Patent 7,457,446) uses line sensors on microscopes to create virtual microscopic images for clinical and educational use by pathologists. In order to combine the linear scan data into a complete virtual microsco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/36G01N21/84
CPCG02B21/36G02B21/002
Inventor 斯蒂芬 立业 陈
Owner 斯蒂芬・立业・陈
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