Electrochemical luminescence method for detecting diethylstilbestrol

A diethylstilbestrol, electrochemical technology, applied in the field of medical detection, can solve the problems of expensive, time-consuming, cumbersome sensitivity and the like, and achieve the effects of simple instrument, high luminous intensity and high sensitivity

Active Publication Date: 2020-04-10
CHANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are usually expensive, time-consuming and cumbersome and have low sensitivity

Method used

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  • Electrochemical luminescence method for detecting diethylstilbestrol
  • Electrochemical luminescence method for detecting diethylstilbestrol
  • Electrochemical luminescence method for detecting diethylstilbestrol

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Embodiment 1

[0037] An electrochemiluminescence method for detecting diethylstilbestrol, the specific steps are as follows:

[0038] (1) Perylenetetracarboxylic acid (PTCA) immobilized aminated Ti-based MOFs (NH 2 -MIL-125) Preparation of composite materials:

[0039] Add 2.4 mL of isopropyl titanate and 2.2 g of 2-aminoterephthalic acid to the mixed solution of 20 mL of DMF and 20 mL of methanol, stir and mix well with ultrasound, pour the resulting yellow milky liquid into a 50 mL autoclave, and dry it in a vacuum Heated in the box at 150℃ for 48h. After the kettle is cooled, it is washed three times with methanol and DMF, centrifuged, and dried at 60℃ to obtain a yellow powder, namely NH 2 -MIL-125.

[0040] Dissolve 0.1g perylenetetracarboxylic dianhydride (PTCDA) in 10mL 0.1M NaOH, stir at 80℃ for 2h to obtain a yellow-green solution, drop 1.0M HCl until complete precipitation, centrifuge and wash with deionized water three times, Dry at 60°C to obtain dark red powder, namely PTCA.

[0041] ...

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Abstract

The invention discloses an electrochemical luminescence method for detecting diethylstilbestrol, and particularly belongs to the field of electrochemical luminescence detection. The operation processcomprises the steps of: (1) preparing a composite material of perylenetetracarboxylic acid (PTCA) immobilized aminated Ti-based MOFs (NH2-MIL-125); (2) preparing an electrochemical luminescence aptamer (apy) sensor; and (3) detecting diethylstilbestrol by using an electrochemical luminescence method, wherein an pt/PTCA/NH2-MIL-125 modified glassy carbon electrode is used as an operating electrode,an Ag/AgCl electrode is used as a reference electrode, a platinum electrode is used as an auxiliary electrode, and a traditional three-electrode system is formed. The detection range of the electrochemical luminescence method is 1.0*10<-15> mol/L to 1.0*10<-6 > mol/L, and the lowest detection limit is 2.8*10<-16> mol/L. The electrochemical luminescence method for detecting diethylstilbestrol is low in cost, high in sensitivity, strong in specificity and simple to operate.

Description

Technical field [0001] The invention belongs to the technical field of medical detection, and relates to an electrochemiluminescence method for detecting diethylstilbestrol. Background technique [0002] With the rapid development of science and technology and people’s increasing material needs, a series of environmental problems have erupted. Environmental Endocrine Disrupting Chemicals (EDCs) are a class of chemical substances with hormone-like effects that exist in the natural environment. They can interfere with the synthesis, secretion, transport and degradation of endogenous hormones in organisms, thereby damaging organisms. The reproductive development, immunity, endocrine and nervous system of the body, and even the endocrine and metabolism disorders of its offspring. [0003] Among them, diethylstilbestrol (DES) is an endocrine disruptor of medical supplies. It is a synthetic non-steroidal estrogenic substance that can produce all the pharmacological and therapeutic effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48G01N21/76
CPCG01N27/30G01N27/48G01N21/76
Inventor 李静娴单学凌蒋鼎陈智栋
Owner CHANGZHOU UNIV
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