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Storage agent, calibrator for detecting LH and detection kit

A storage agent and calibrator technology, applied in the field of storage agents, can solve the problems of complex production process, difficult preservation of calibrators, poor stability of LH antigen, etc.

Pending Publication Date: 2020-04-10
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the LH antigen is very unstable and difficult to store in solution
Therefore, the calibrator in the immunoassay needs to be prepared on site, especially the calibrator of LH in the detection kit is difficult to provide directly in the form of solution
Many manufacturers' calibrators are in the form of freeze-dried products, but the use of freeze-dried products leads to more complicated production processes and correspondingly cumbersome detection steps
In addition, in addition to the problem of LH, there are also some common detection items that are difficult to store in solution for a long time.

Method used

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  • Storage agent, calibrator for detecting LH and detection kit
  • Storage agent, calibrator for detecting LH and detection kit
  • Storage agent, calibrator for detecting LH and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0197] Prepare the calibrator with the storage agent (pH7.4) of the present invention, the formula of the used storage agent of the calibrator of the present embodiment is as follows:

[0198]

[0199] Calibrators with different LH concentrations (0 (control), 0.2, 1, 5, 20, 100, 200 IU / L) were prepared respectively for use.

Embodiment 2

[0213] Prepare calibrator with storage agent (pH6.0) of the present invention, the calibrator formula of the present embodiment is as follows:

[0214]

[0215]

[0216] Calibrators with different LH concentrations (0 (control), 0.2, 1, 5, 20, 100, 200 IU / L) were prepared respectively for use.

Embodiment 3～4

[0223] Prepare calibrator with storage agent (pH7.4) of the present invention, the calibrator formula of present embodiment 3 and 4 is as follows respectively:

[0224]

[0225]

[0226] Calibrators of Example 3 and Example 4 with different LH concentrations (0 (control), 0.2, 1, 5, 20, 100, 200 IU / L) were prepared respectively for use.

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Abstract

The invention discloses a storage agent, a calibrator for detecting LH and a detection kit. The storage agent is used for stably storing antigens, and comprises 10-50 mmol / L of a buffer solution witha pH value of 5.5-8, 0.5-3 g / L of 8-aniline-1-naphthalene sulfonic acid and / or a salt thereof, 5-70 g / L of a protective agent, and 0.05-1 mL / L of a surfactant. The storage agent provided by the invention can stably store a plurality of antigens, shows excellent signal retention rate in an accelerated stability test, solves the problem that LH cannot be stored in a solution form for a long time, and avoids the problem of tedious detection steps caused by providing a calibration product in a freeze-drying form.

Description

technical field [0001] The invention relates to a storage agent for stably preserving antigens in biochemical detection, in particular to a calibrator and a kit for stable storage in the detection of luteinizing hormone. Background technique [0002] Luteinizing hormone (LH) is a glycoprotein hormone synthesized and secreted by the anterior pituitary gland and consists of two subunits, α and β. LH synthesis is regulated by sex hormone-releasing hormone (GnRH) in the hypothalamus, with feedback from steroid sex hormones. LH stimulates female ovulation and ovaries to secrete estrogen and progesterone; controls male testicular Leydig cells to secrete testosterone. In women, LH content is low during the follicular phase; it peaks in the middle of menstruation, triggering ovulation, and drops to a minimum after ovulation. LH is highest in postmenopausal women, even higher than the midmenstrual LH peak; LH levels are also high in gonadically ablated men. LH is important for the...

Claims

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Application Information

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IPC IPC(8): G01N33/76G01N33/577G01N33/543G01N33/531G01N21/78
CPCG01N33/76G01N33/577G01N33/54326G01N33/531G01N21/78
Inventor 胡容张凌燕
Owner SICHUAN MACCURA BIOTECH CO LTD
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