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Polymorphic Molecular Markers and Their Applications for Differentiation of Xinlu Zhong Series Cotton Varieties

A polymorphism marker and molecular marker technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve the effects of high accuracy, strong timeliness, and stable variation

Active Publication Date: 2022-05-03
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, most studies on the fingerprints of upland cotton varieties in Xinjiang focus on extra-early and early-maturing cotton varieties, and the construction of fingerprints for early-medium-maturing upland cotton varieties needs to be improved

Method used

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  • Polymorphic Molecular Markers and Their Applications for Differentiation of Xinlu Zhong Series Cotton Varieties
  • Polymorphic Molecular Markers and Their Applications for Differentiation of Xinlu Zhong Series Cotton Varieties
  • Polymorphic Molecular Markers and Their Applications for Differentiation of Xinlu Zhong Series Cotton Varieties

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Embodiment 1

[0117] 1. materials

[0118] Unless otherwise specified, the methods used in this example are conventional methods known to those skilled in the art, and the reagents and other materials used are commercially available products unless otherwise specified.

[0119] 2. Methods

[0120] According to the invention, the screened core primers with high polymorphism, good stability and strong discrimination ability are used, and 80 Xinluzhong series cotton varieties are used as test materials (Table 1) to construct DNA fingerprints of Xinluzhong series cotton varieties, so as to obtain molecular markers which can be used for identifying 80 Xinluzhong series cotton varieties. A total of 14 characteristic primers (Table 2) and 66 combination primers (Table 3) of new land varieties were obtained. At least 19 pairs of core primers can be used to completely distinguish 80 Xinluzhong cotton varieties, and the decimal digital fingerprint codes of 80 Xinluzhong cotton varieties under 19 pairs of...

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Abstract

The invention relates to a polymorphic molecular marker for identifying a series of cotton varieties in Xinluzhong and its application, and belongs to the technical field of molecular detection. The invention uses 29 pairs of polymorphic identification primers screened to realize the identification of 80 varieties of Xinluzhong Fingerprint construction and molecular identification of a series of cotton varieties, the 29 pairs of primers have the advantages of high polymorphism, good stability, simple operation, high accuracy, and good versatility; the present invention only needs 19 pairs of core primers to identify 80 A new series of cotton varieties in the land.

Description

Technical field [0001] The invention belongs to the technical field of molecular detection, and in particular relates to a polymorphic molecular marker for identifying a series of cotton varieties in Xinluzhong and its application. technical background [0002] Cotton is an important cash crop rich in fiber. Xinjiang is the main cotton producing area in China, with its planting area of about 50% contributing nearly 3 / 4 of the output, which plays an important role in ensuring the safety of cotton production in China. In recent years, with the rapid development of the cotton industry, the phenomenon of "many, chaotic, mixed and miscellaneous" in the cotton seed market has become increasingly serious, and the problems of cotton variety homogenization and brand registration are prominent, which seriously affect the healthy development of the cotton industry. In addition, the repeated utilization of backbone parents and the application of transgenic technology in cotton breeding have ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 薛飞李婧慧金彦龙孙杰刘峰张新宇王静
Owner SHIHEZI UNIVERSITY