Quantitative detection device of protein interaction under control of membrane potential

Abstract

The invention discloses a quantitative detection device of protein interaction under control of the membrane potential. Hollows at two sides of an experimental containing cavity are used for a micropipette and a glass electrode to enter; cells in a semi-adherent state are on the bottom surface of the experimental containing cavity; the micropipette absorbs red blood cells; pippette solution is filled in the glass electrode; a patch clamp probe is fixed on an experimental platform through a patch clamp probe bracket; the recording electrode of the patch clamp probe is connected to the tail endof a glass electrode clamping device; the glass electrode is connected to the clamping end of the glass electrode clamping device; a reference electrode is connected into extracellular fluid in the experimental containing cavity; a bright-field mercury lamp light source is arranged over an inverted microscope; and the bright-field light path of the bright-field mercury lamp light source irradiatesthe experimental containing cavity, and then, is received by an industrial camera. According to the quantitative detection device of the protein interaction under control of the membrane potential inthe invention, influence of membrane potential change onto the dynamic function of membrane proteins can be directly detected; and furthermore, the coupling relationship of two pieces of spectral phase information can be recorded while the two pieces of spectral phase information are synchronously detected.

Description

technical field [0001] The invention relates to the integration of the patch clamp technology and the micropipette technology, in particular to a quantitative detection device for protein molecule interaction under the regulation of membrane potential. Background technique [0002] The membrane potential of the cell membrane is a key regulator of cell life activities. The membrane potential of neurons plays an important role in regulating the life activities of neurons, and is an important biophysical factor that dynamically regulates the structure and function of brain neural networks; in non-neuronal cells, the membrane potential of cells also regulates their proliferation , differentiation and other life activities. Patch clamp technology is an effective means to study membrane potential-related issues, and its whole-cell recording mode can realize accurate and rapid control of the membrane potential of the entire cell membrane. However, patch clamping is currently larg...

AI Technical Summary

Problems solved by technology

However, there is currently no method to study the dynamic regulation of membrane potential changes on the interaction between membrane proteins under physiological conditions.

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