Lactobacillus plantarum DNB1 and extracellular polysaccharide and application thereof

A technology of Lactobacillus plantarum and extracellular polysaccharides, applied in the field of microorganisms, can solve the problems that lactic acid bacteria cannot regulate intestinal flora and anti-oxidation at the same time, and application limitations, and achieve significant in vitro anti-oxidation ability, high survival rate, and good tolerance affected by performance

Active Publication Date: 2020-05-05
中科利君股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the existing Lactobacillus plantarum needs to be compounded with other probiotics to regulate the intestinal flora, or the Lactobacillus plantarum only has anti-oxidation function.

Method used

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  • Lactobacillus plantarum DNB1 and extracellular polysaccharide and application thereof
  • Lactobacillus plantarum DNB1 and extracellular polysaccharide and application thereof
  • Lactobacillus plantarum DNB1 and extracellular polysaccharide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0056] Example 1 Isolation and identification of Lactobacillus plantarum DNB1

[0057] 1. Strain isolation and screening

[0058] Take the natural fermentation broth of grapefruit peel under aseptic conditions from 10mL to 90mL of sterile physiological saline, pipette evenly, and dilute it to an appropriate concentration by a 10-fold gradient, then take 100μL of the diluted bacterial solution and use a spreading rod to evenly Spread on the MRS solid medium and invert it in a constant temperature incubator at 37°C for 48 hours. Pick a single colony with the typical characteristics of lactic acid bacteria (round, milky white, neat edges, convex), and repeat the streaking and separation on the MRS solid medium to obtain a single colony;

[0059] Pick a single colony with a sterile bamboo stick, gently pull it out, measure the length of the colony drawing, and select the strain with the largest drawing length as the target strain.

[0060] 2. Identification of target strains

[0061] (1) ...

Example Embodiment

[0074] Example 2 Determination of acid and bile salt resistance of Lactobacillus plantarum DNB1

[0075] 1. Determination of acid tolerance of Lactobacillus plantarum DNB1

[0076] The acid resistance of lactic acid bacteria is one of the necessary conditions to ensure their survival through the gastric juice into the intestine, so the acid tolerance of lactic acid bacteria is an important indicator.

[0077] (1) Experimental method

[0078] Inoculate Lactobacillus plantarum DNB1 into MRS liquid medium, activate 3 times, culture in a constant temperature incubator at 37°C for 24 hours, centrifuge at 3000r / min and 4°C for 10 minutes to collect the bacteria, and wash the bacteria with an equal volume of sterile saline 2 times, centrifuge under the same conditions, pour out the supernatant, and suspend the bacteria in an equal volume of sterile saline to prepare the test bacteria liquid;

[0079] Inoculate the tested bacteria solution into MRS liquid medium with pH values ​​of 2.0, 3.0, 4...

Example Embodiment

[0104] Example 3 Extraction of Lactobacillus plantarum DNB1 extracellular polysaccharide and determination of yield

[0105] 1. Experimental method

[0106] (1) Extraction of extracellular polysaccharide of Lactobacillus plantarum DNB1

[0107] The extraction method of Lactobacillus plantarum DNB1 extracellular polysaccharide includes the following steps:

[0108] S1. Inoculate Lactobacillus plantarum DNB1 into MRS liquid medium at an inoculum of 3% (v / v), culture in a constant temperature incubator at 37°C for 24 hours, and after continuous activation for 3 times, centrifuge at 4°C and 10000r / min for 10 minutes Remove the bacteria and impurities in the fermentation broth to obtain the fermentation broth;

[0109] S2. Add 80% trichloroacetic acid solution to the fermentation broth so that the final concentration of trichloroacetic acid in the solution is 4% (m / v). After placing it in a refrigerator at 4°C for 7 hours, centrifuge at 4°C and 10000r / min for 15 minutes Remove the protein ...

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Abstract

The invention discloses lactobacillus plantarum DNB1 and an extracellular polysaccharide and application thereof. The lactobacillus plantarum DNB1 is obtained by separating and screening from naturalfermentation liquor of pomelo peel, and was deposited in Guangdong Microbial Culture Collection Center on July 15, 2019 with a deposit number of GDMCC No. 60728. The strain has good tolerance to acidand bile salt, and has good adaptability to the environment of gastrointestinal fluid, and a fermentation broth of the strain has a significant bacteriostatic effect. The extracellular polysaccharideproduced by the strain has significant in vitro antioxidant capacity, significantly improves glutathione oxidase activity and total antioxidant capacity in the blood, can regulate relative abundance of Lactobacillus in the intestinal flora and reduce relative abundance of Staphylococcus; therefore, extracellular polysaccharide has good popularization and application prospects in regulating intestinal flora or preparing intestinal flora regulators, improving antioxidant capacity or preparing antioxidants.

Description

technical field [0001] The invention belongs to the technical field of microorganisms. More specifically, it relates to a strain of Lactobacillus plantarum DNB1 and its exopolysaccharide and application. Background technique [0002] Lactic acid bacteria exopolysaccharides (Exopoly Saccharides, EPS) refers to the general term for mucus polysaccharides or capsular polysaccharides secreted by lactic acid bacteria outside the cell wall during the growth process. It has the characteristics of large molecular weight, high viscosity, and complex structure. The lactic acid bacteria that produce exopolysaccharides in nature It mainly includes Lactobacillus casei, Lactobacillus delbrueckii, Leuconostoc enterococci and Lactobacillus plantarum. Studies have shown that lactic acid bacteria exopolysaccharide is a safe natural product, which can improve the rheology, tissue state, texture characteristics and sensory flavor of fermented dairy products, making it more delicate, uniform, sm...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04A61K31/715A61P39/06A61P1/00C12R1/25
CPCC12N1/20C12P19/04A61K31/715A61P39/06A61P1/00C12R2001/25C12N1/205
Inventor 钟青萍上官文丹丁楠卢承蓉方祥廖振林王洁
Owner 中科利君股份有限公司
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