Centipeda minima polysaccharide as well as preparation method and application thereof
A kind of technology for geese not to eat grass and polysaccharide, applied in the field of geese not to eat grass and polysaccharide and preparation thereof
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0045] Example 1: Preparation of goose herbivorous polysaccharide CMP-2B
[0046] (1) Polysaccharide extraction:
[0047] Take 1000g of washed and dried geese that do not eat grass, pass through a 60-mesh sieve after being crushed by a pulverizer, add 6L of absolute ethanol and reflux for two hours in a water bath at 70°C, repeat the reflux twice for degreasing and decolorization, filter, and filter the residue at 45°C Dried in a blast oven, added 20L of water, leached for 4 hours in a water bath at 80°C, centrifuged, filtered, concentrated to 2.5L, and slowly added four times the volume of 10L of absolute ethanol at a speed of 500r / min , stood still at -4°C for 12 hours, centrifuged, collected the precipitate, and washed three times with absolute ethanol and acetone in turn, and freeze-dried the precipitate to obtain 23.6 g of crude polysaccharide from Goose Bubivora.
[0048] (2) In addition to protein:
[0049] Take 20 g of the goose non-herbivorous crude polysaccharide p...
Embodiment example 2
[0066] Implementation Case 2: Antioxidative Activity Experiment of Goose Herbivorous Polysaccharide CMP-2B
[0067] The antioxidant activity of polysaccharides was evaluated by DPPH, ABTS method and superoxide free radical experiment, as follows:
[0068] Scavenging DPPH free radicals: Mix 150 μL CMP-2B aqueous solution (0.25, 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mg / mL) and 150 μL DPPH ethanol solution (60 μmol / L) in a 96-well plate, keep at room temperature and avoid light React for 30min, test the absorbance value of the mixture at 517nm, take ascorbic acid (Vc) as positive control, see the results Figure 8 a.
[0069] Scavenging ABTS free radicals: Mix 2 mL of ABTS (7 mmol / L) aqueous solution (with 2 mL of potassium persulfate aqueous solution (2.45 mmol / L) and react in the dark for 12 hours at room temperature to produce cationic ABTS free radicals. The cationic ABTS The free radical solution was diluted with ethanol until the absorbance at 734nm was 0.7±0.05. Add 20 μL of ...
Embodiment example 3
[0072] Implementation case 3: Goose non-herbivorous polysaccharide CMP-2B inhibits α-glucosidase activity experiment
[0073] Add 20 μL polysaccharide aqueous solution (0.1, 0.25, 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mg / mL) and 40 μL α-glucosidase aqueous solution (0.2 U / mL) to a 96-well plate, mix well, and store at 37 ° C Incubate for 10 min, then add 20.0 μL pNPG aqueous solution (10 mmol / L), mix well, react at 37 °C for 30 min, and finally add 100 μL Na 2 CO 3 (0.2mol / L) solution to terminate the reaction, and the absorbance of the mixture was tested at 405nm, and acarbose was used as a positive control. The results are shown in FIG. 11 .
[0074] The results showed that goose non-herbivorous polysaccharide CMP-2B had inhibitory activity of α-glucosidase in the concentration range of 0.1-3.0mg / mL, and its inhibitory activity was enhanced with the increase of concentration. When the concentration was 3.0mg / mL, the inhibition rate of CMP-2B was 90.03±1.49%. It shows that goos...
PUM
| Property | Measurement | Unit |
|---|---|---|
| molecular weight | aaaaa | aaaaa |
| molecular weight | aaaaa | aaaaa |
| absorbance | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More