Hyperspectral rapid imaging measurement system for molecular vibration mode

A molecular vibration and imaging measurement technology, applied in measurement devices, analytical materials, material excitation analysis, etc., can solve the problems of long imaging time, few scanning pixels, unable to meet the rapid imaging of living biological samples, etc., to meet imaging requirements, improve Imaging speed, the effect of fast hyperspectral imaging

Active Publication Date: 2020-05-08
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the point-by-point scanning imaging method, this method still has the disadvantages of few scanning pixels and long imaging time, which cannot meet the requirements of rapid imaging of living biological samples.

Method used

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  • Hyperspectral rapid imaging measurement system for molecular vibration mode
  • Hyperspectral rapid imaging measurement system for molecular vibration mode
  • Hyperspectral rapid imaging measurement system for molecular vibration mode

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Such as figure 1 As shown, it is a hyperspectral imaging measurement system for molecular vibrational modes according to Embodiment 1 of the present invention, including: an optical comb light source, used to generate pump light as a wide-field coherent Raman scattering process, Stokes Light and probe light; wide-field coherent Raman scattering microscopic optical path, used to form the light generated by the optical comb light source into a large spot beam to irradiate the sample, so that the sample generates anti-Stokes light under the effect of coherent Raman scattering signal; and a high-speed area array photodetector for collecting anti-Stokes light signals from the sample and performing fast hyperspectral imaging.

[0028] The optical comb light source is composed of a single optical comb 11, a beam splitter 13 and a Michelson interferometer 14 with a delay line device for controlling phase. The light generated by the single optical comb 11 is split by the beam s...

Embodiment 2

[0034] Such as figure 2 As shown, it is a hyperspectral imaging measurement system for molecular vibrational modes according to Embodiment 2 of the present invention, including: an optical comb light source, used to generate pump light as a wide-field coherent Raman scattering process, Stokes Light and probe light; wide-field coherent Raman scattering microscopic optical path, used to form the light generated by the optical comb light source into a large spot beam to irradiate the sample, so that the sample generates anti-Stokes light under the effect of coherent Raman scattering signal; and a high-speed area array photodetector for collecting anti-Stokes light signals from the sample and performing fast hyperspectral imaging.

[0035] The difference from Embodiment 1 is that the optical comb light source in this embodiment is composed of dual optical combs 21 (namely, optical comb 1 and optical comb 2 ), reflecting mirror 22 , and beam splitter 23 . The repetition frequency...

Embodiment 3

[0040] Such as image 3 As shown, it is a hyperspectral imaging measurement system for molecular vibrational modes according to Embodiment 3 of the present invention, including: an optical comb light source, used to generate pump light as a wide-field coherent Raman scattering process, Stokes Light and probe light; wide-field coherent Raman scattering microscopic optical path, used to form the light generated by the optical comb light source into a large spot beam to irradiate the sample, so that the sample generates anti-Stokes light under the effect of coherent Raman scattering signal; and a high-speed area array photodetector for collecting anti-Stokes light signals from the sample and performing fast hyperspectral imaging.

[0041] The optical comb light source in this embodiment is a dual optical comb light source, which is the same as that in Embodiment 2, and will not be repeated here.

[0042] In this embodiment, different from Embodiments 1 and 2, according to differ...

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Abstract

The invention provides a hyperspectral rapid imaging system for a molecular vibration mode. The hyperspectral rapid imaging system comprises an optical comb light source, a wide-field coherent Raman scattering microscopic light path and a high-speed area array photoelectric detector, wherein the optical comb light source is used for generating pump light, Stokes light and detection light in a wide-field coherent Raman scattering process; the wide-field coherent Raman scattering microscopic light path is used for forming light generated by means of the optical comb light source into a large-light-spot light beam to irradiate a sample, so that the sample generates an anti-Stokes light signal under the action of a coherent Raman scattering effect; and the high-speed area array photoelectric detector is used for collecting the anti-Stokes optical signal emitted from the sample and carrying out rapid hyperspectral imaging. By adopting the hyperspectral rapid imaging system, spectral measurement and imaging can be carried out at the same time, the imaging speed is greatly accelerated, and the imaging requirement of a living biological sample is met.

Description

technical field [0001] The invention relates to the field of coherent Raman scattering microscopic imaging, in particular to a hyperspectral fast imaging measurement system for molecular vibration modes. Background technique [0002] Coherent Raman scattering is a nonlinear optical process based on molecular vibrational energy levels. It has fingerprint recognition ability for the vibration and rotation of molecular functional groups. It is very suitable for label-free biological imaging, and has higher spatial resolution. It mainly includes coherent anti-Stokes Raman scattering (Coherent Anti-Stokes Raman Scattering, CARS) and stimulated Raman scattering (Stimulated Raman Scattering, SRS). Compared with spontaneous Raman scattering, it greatly enhances the Raman signal through coherent excitation and has higher detection sensitivity. [0003] Coherent anti-Stokes Raman scattering is a third-order nonlinear optical process that greatly enhances the sensitivity of single-mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N21/01
CPCG01N21/65G01N21/01G01N2021/0112Y02A40/10
Inventor 张三军闫明田阳武愕周黄梅
Owner EAST CHINA NORMAL UNIV
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