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A kind of tissue culture method of Ganoderma lucidum

A tissue culture and Ganoderma lucidum technology, applied in the directions of botanical equipment and methods, cultivation, plant cultivation, etc., can solve the problems of low explant survival rate, low content of amino acids and polysaccharides, and low growth rate of mycelium, to prevent The effect of cell senescence, enhancement of stress resistance, and improvement of survival rate

Active Publication Date: 2021-09-28
GUANGXI XIANCAOTANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a tissue culture method for Ganoderma lucidum in view of the problems of low survival rate of explants, low growth rate of mycelium, and low content of amino acids and polysaccharides in the existing tissue culture method of Ganoderma lucidum

Method used

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  • A kind of tissue culture method of Ganoderma lucidum
  • A kind of tissue culture method of Ganoderma lucidum

Examples

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Embodiment 1

[0030] A method for tissue culture of Ganoderma lucidum, comprising the following steps:

[0031] a. Take the fruiting bodies of Ganoderma lucidum, soak them in the biological disinfectant on the ultra-clean bench for disinfection, the soaking time is 60 seconds, and then take them out;

[0032] Wherein, the biological disinfectant solution includes the following raw materials in parts by weight: 5 parts of oryzanol, 12 parts of chitosan, 10 parts of arachidonic acid, 0.01 part of Bacillus amyloliquefaciens, 0.03 part of isothiocyanate, and 0.01 part of artemisinin , 0.02 parts of kasugamycin, 0.08 parts of astaxanthin and 100 parts of water.

[0033] The preparation method of biological disinfectant is as follows: take oryzanol, chitosan, arachidonic acid and Bacillus amyloliquefaciens, add water and stir evenly, cultivate at 20°C for 80 hours, then take it out, add isothiocyanate, artemisinin, Kasugamycin and astaxanthin were stirred evenly, and then sterilized by filtratio...

Embodiment 2

[0043] A method for tissue culture of Ganoderma lucidum, comprising the following steps:

[0044] a. Take the fruiting bodies of Ganoderma lucidum, soak them in the biological disinfectant on the ultra-clean bench for disinfection, the soaking time is 120 seconds, and then take them out;

[0045] Wherein, the biological disinfectant solution includes the following raw materials in parts by weight: 15 parts of oryzanol, 24 parts of chitosan, 20 parts of arachidonic acid, 0.05 part of Bacillus amyloliquefaciens, 0.07 part of isothiocyanate, and 0.04 part of artemisinin , 0.07 parts of kasugamycin, 0.14 parts of astaxanthin and 200 parts of water.

[0046] The preparation method of the biological disinfectant is as follows: take oryzanol, chitosan, arachidonic acid and Bacillus amyloliquefaciens, add water and stir evenly, cultivate at 30°C for 100 hours, then take it out, add isothiocyanate, artemisinin, Kasugamycin and astaxanthin were stirred evenly, and then sterilized by fi...

Embodiment 3

[0056] A method for tissue culture of Ganoderma lucidum, comprising the following steps:

[0057] a. Take the fruiting bodies of Ganoderma lucidum, soak them in the biological disinfectant on the ultra-clean bench for disinfection, the soaking time is 75 seconds, and then take them out;

[0058] Wherein, the biological disinfectant includes the following raw materials in parts by weight: 7 parts of oryzanol, 15 parts of chitosan, 12 parts of arachidonic acid, 0.02 part of Bacillus amyloliquefaciens, 0.04 part of isothiocyanate, and 0.02 part of artemisinin , 0.03 parts of kasugamycin, 0.01 parts of astaxanthin and 125 parts of water.

[0059] The preparation method of biological disinfectant is as follows: take oryzanol, chitosan, arachidonic acid and Bacillus amyloliquefaciens, add water and stir evenly, cultivate at 22°C for 85 hours, then take it out, add isothiocyanate, artemisinin, Kasugamycin and astaxanthin were stirred evenly, and then sterilized by filtration with a ...

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Abstract

The invention provides a method for tissue culture of Ganoderma lucidum, and specifically relates to the technical field of edible fungus tissue culture. The present invention makes a breakthrough by focusing on the polyphenol oxidase in the fruit body tissue of Ganoderma lucidum. Before cutting the explants, use a biological disinfectant to reduce the activity of polyphenol oxidase in the fruit body to prevent browning, and then obtain After the explants are refrigerated and activated with fresh-keeping solution, the cellulase and polyphenolase in the explants are activated to promote the rapid growth of hyphae after inoculation of the explants, and red light and green light of specific wavelengths are used alternately during the cultivation period , Intermittent irradiation, and then try it with microwave irradiation to stimulate the growth of mycelium and the enrichment of active ingredients in it. The explant survival rate of the present invention is more than 72%, the obtained mycelium growth rate is 8mm / d, the total amino acid content is more than 16%, and the polysaccharide content is more than 4%.

Description

【Technical field】 [0001] The invention relates to the technical field of edible fungus tissue culture, in particular to a method for tissue culture of Ganoderma lucidum. 【Background technique】 [0002] Ganoderma lucidum has always been regarded as an elite plant in China, and has the reputation of "auspicious grass" and "immortal grass". It is called Wannian antler in Japan and is a precious medicinal (edible) fungus. "Shen Nong's Materia Medica" points out: Chizhi (Ganoderma lucidum) is "bitter, flat, and non-toxic", and it is mainly used to treat "chest knots, benefit the heart, nourish the middle, increase wisdom, and not forget", "long-term food makes light of body and does not grow old, prolongs life and immortality" . "Compendium of Materia Medica" records that Ganoderma lucidum has miraculous effects such as "nourishing and strengthening, prolonging life, benefiting joints, and curing deafness", and lists it as a top-grade medicine. [0003] In nature, Ganoderma luc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/20A01G18/30A01G18/40A01G18/69A01N37/38A01N43/16A01N37/06A01N63/22A01N47/46A01N43/90A01N49/00A01P3/00A01P1/00A01N3/00
CPCA01N3/00A01N37/06A01N37/38A01N43/16A01N43/90A01N47/46A01N49/00A01G18/00A01G18/20A01G18/30A01G18/40A01G18/69
Inventor 覃华兴刘长斯韦孟宝梁李韦珍
Owner GUANGXI XIANCAOTANG PHARMA
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