A kind of fresh Cordyceps sinensis quick-frozen preservation method with liquid nitrogen combined with liquid carbon dioxide
A technology of liquid carbon dioxide and Cordyceps sinensis, applied in food preservation, climate change adaptation, food science, etc., can solve the problems of decreased freshness of Cordyceps sinensis, low polyphenol oxidase activity, high soluble protein content, etc. Effect of low phenoloxidase activity and high soluble protein content
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[0049] After obtaining the quick-frozen Cordyceps sinensis, the present invention sprays a preservative on the quick-frozen Cordyceps sinensis; the preservative includes 30-100 mg / mL Cordyceps mycelium extract, 50-200 mg / L chitosan oligosaccharide, 100-200 mg / L Antimicrobial Peptides and Water. In the present invention, the function of the fresh-keeping agent is to inhibit the growth of bacteria on the surface of the sample and prevent the sample from spoiling during the fresh-keeping process. The antistaling agent of the present invention preferably includes 50-70 mg / mL of Cordyceps mycelium extract. In the present invention, the function of the Cordyceps mycelium extract is an antibacterial antistaling agent, which inhibits the growth of bacteria during the preservation process. In the present invention, the Cordyceps mycelium extract includes guanine, cordycepin, hypoxanthine, cytidine, xanthine and adenosine. In the present invention, the preparation method of the Cordyc...
Embodiment 1
[0058] (1) Washing with running water: Clean the head, sporocarpus and worm body of the Cordyceps sinensis with soil collected from the place of production with linear running water at a temperature of 0°C.
[0059] (2) Ultrasonic cleaning: place the cleaned Cordyceps sinensis in a 0.1% calcium lactate solution, ultrasonically clean 5 times (power 250W, frequency 40k Hz, temperature 0°C, time 5min / time), drain .
[0060] (3) Soaking: Soak in cryoprotectant for 2 minutes, cryoprotectant: 0.3mol / L propylene glycol, 1mmol / L sorbitol, 1mmol / L Vc, 6μmol / L phytic acid, L-cysteine at a concentration of 0.4mg / kg.
[0061] (4) Quick-freezing: Use a liquid nitrogen tunnel quick-freezing machine, the temperature of the freezing room is -120°C, spray liquid nitrogen and acetic acid / citric acid mist liquid into the freezing room, control the pH value of the freezing room to 4, and keep the material from entering and exiting for 5 minutes. When the material is exported, it is continuou...
Embodiment 2
[0066] (1) Cleaning with running water: Clean the head, sporocarpus and worm bodies of the Cordyceps sinensis with soil collected in the production area sequentially with linear running water at a temperature of 10°C.
[0067] (2) Ultrasonic cleaning: the cleaned Cordyceps sinensis is placed in a 0.5% calcium lactate solution, ultrasonically cleaned once (power 1200W, frequency 120Hz, temperature 10°C, time 10min / time), and drained.
[0068] (3) Soaking: Soak in cryoprotectant for 5 minutes, cryoprotectant: 0.5mol / L propylene glycol, 3mmol / L sorbitol, 3mmol / LVc, 9μmol / L phytic acid, L-cysteine at a concentration of 0.8mg / L kg.
[0069] (4) Quick-freezing: Use a liquid nitrogen tunnel quick-freezing machine, the temperature of the freezing room is -90°C, spray liquid nitrogen and acetic acid / citric acid mist liquid into the freezing room, control the pH value of the freezing room to 6, and keep the material from entering and exiting for 10 minutes. When the material is exporte...
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