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A liquid nitrogen spray type quick-freezing method for fresh Cordyceps sinensis

A technology for fresh Cordyceps sinensis and Cordyceps sinensis is applied in the field of liquid nitrogen spray type quick freezing of fresh Cordyceps sinensis, which can solve the problems of decreased freshness of Cordyceps sinensis, high soluble protein content, low polyphenol oxidase activity, etc., and achieves low weight loss rate and soluble protein content. The effect of high, low polyphenol oxidase activity

Active Publication Date: 2022-02-01
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method provided by the invention can solve the problem that the freshness of Cordyceps sinensis decreases in the preservation process, the weight loss rate is low, the soluble protein content is high and the activity of polyphenol oxidase is low

Method used

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  • A liquid nitrogen spray type quick-freezing method for fresh Cordyceps sinensis

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preparation example Construction

[0045] After obtaining the quick-frozen Cordyceps sinensis, the present invention sprays a preservative on the quick-frozen Cordyceps sinensis; the preservative includes 30-100 mg / mL Cordyceps mycelium extract, 50-200 mg / L chitosan oligosaccharide, 100-200 mg / L Antimicrobial Peptides and Water. In the present invention, the function of the fresh-keeping agent is to inhibit the growth of bacteria on the surface of the sample and prevent the sample from spoiling during the fresh-keeping process. The antistaling agent of the present invention preferably includes 50-70 mg / mL of Cordyceps mycelium extract. In the present invention, the function of the Cordyceps mycelium extract is an antibacterial antistaling agent, which inhibits the growth of bacteria during the preservation process. In the present invention, the Cordyceps mycelium extract includes guanine, cordycepin, hypoxanthine, cytidine, xanthine and adenosine. In the present invention, the preparation method of the Cordyc...

Embodiment 1

[0054] (1) Washing with running water: Clean the head, sporocarpus and worm body of the Cordyceps sinensis with soil collected from the place of production with linear running water at a temperature of 0°C.

[0055] (2) Ultrasonic cleaning: the cleaned Cordyceps sinensis was placed in a 0.5% calcium lactate solution, ultrasonically cleaned 5 times (power 250W, frequency 120Hz, temperature 0°C, time 5min / time), and drained.

[0056] (3) Soaking: Soak in cryoprotectant for 2min, cryoprotectant: 0.5mol / L propylene glycol, 3mmol / L sorbitol, 3mmol / L iso-Vc, 9μmol / L phytic acid, L-cysteine ​​at a concentration of 0.8 mg / kg.

[0057] (4) Quick-freezing: use a liquid nitrogen spray quick-freezing machine, multi-stage freezing procedure, drop from ambient temperature to -20°C in 1 minute, drop to -60°C in 2 minutes, and keep the temperature for 15 minutes.

[0058] (5) Spray preservative: containing 100mg / mL Cordyceps mycelium extract, 200mg / L chitosan oligosaccharide, 200mg / L antimic...

Embodiment 2

[0062] (1) Cleaning with running water: Clean the head, sporocarpus and worm bodies of the Cordyceps sinensis with soil collected in the production area sequentially with linear running water at a temperature of 10°C.

[0063] (2) Ultrasonic cleaning: place the cleaned Cordyceps sinensis in a 0.1% calcium lactate solution, ultrasonically clean once (power 1200W, frequency 40k HzHz, temperature 10°C, time 10min / time), drain .

[0064] (3) Soaking: Soak in cryoprotectant for 5 minutes, cryoprotectant: 0.3mol / L propylene glycol, 1mmol / L sorbitol, 1mmol / L iso-Vc, 6μmol / L phytic acid, L-cysteine ​​at a concentration of 0.4 mg / kg.

[0065] (4) Quick-freezing: use a liquid nitrogen spray quick-freezing machine, multi-stage freezing procedure, drop from ambient temperature to -40°C in 3 minutes, drop to -90°C in 4 minutes, and keep the temperature for 10 minutes.

[0066] (5) Spray preservative: containing 30mg / mL Cordyceps mycelium extract, 50mg / L chitosan oligosaccharide, 100mg / L ...

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Abstract

The invention relates to a liquid nitrogen spraying method for quick-freezing fresh Cordyceps sinensis, which belongs to the technical field of preservation of Cordyceps sinensis. The method provided by the invention comprises the following steps: 1) cleaning the Cordyceps sinensis with water; 2) ultrasonically cleaning the cleaned Cordyceps sinensis; 3) soaking the Cordyceps sinensis in a cryoprotectant; 4) placing the Cordyceps sinensis soaked in step 3) in liquid nitrogen performing multi-stage quick-freezing in the spray quick-freezing device; 5) spraying a preservative on the quick-frozen Cordyceps sinensis; 6) performing modified atmosphere packaging on the sprayed Cordyceps sinensis; 7) refrigerating the modified atmosphere-packed Cordyceps sinensis. The method provided by the invention can solve the problem that the freshness of the Cordyceps sinensis decreases during the preservation process, and the weight loss rate is low, the content of soluble protein is high and the activity of polyphenol oxidase is low.

Description

technical field [0001] The invention relates to the technical field of fresh-keeping Cordyceps sinensis, in particular to a method for quick-freezing fresh Cordyceps sinensis by liquid nitrogen spraying. Background technique [0002] Cordyceps sinensis is a high-grade nourishing and precious Chinese medicinal material, which contains a variety of nutrients and active ingredients, including nucleosides, polysaccharides, sterols, fatty acids, proteins, peptides and volatile components, etc. It has anti-tumor, immune system regulation, and promotes body cell damage Repair, anti-free radicals, anti-oxidation, anti-aging, protect cardiac muscle and vascular cells, improve cardiovascular function, protect the liver, protect the kidneys, regulate the respiratory system, regulate the blood system and regulate blood lipids and other functions. [0003] Most of the Cordyceps currently on the market are dried Cordyceps. During the drying process of Cordyceps, a large number of volatile...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L3/375A23L3/349A23L3/3562A23L3/3544A23L3/3508A23L3/3535A23L3/3463A23L3/3526A23L3/358
Inventor 毕宏涛魏立新肖远灿杜玉枝杨红霞
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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