Portable convection PCR amplification detection device with vibration function

Abstract

The invention discloses a portable convection PCR amplification detection device with a vibration function. The portable convection PCR amplification detection device mainly comprises a temperature control module, a photoelectric detection module, a movement module, a vibrating module and a control module, wherein the temperature control module comprises an upper layer heating module, an upper layer heating resistor film, an upper layer temperature measurement resistor, an upper layer perpendicular radiating fin, a lower layer heating module, a lower layer heating resistor film, a lower layertemperature measurement resistor and a lower layer perpendicular radiating fin. According to the portable convection PCR amplification detection device disclosed by the invention, the upper end and the lower end of a capillary reaction tube for convection PCR amplification are heated to different temperatures, and are kept in a constant temperature, the temperature condition is provided for a convection PCR reaction; and the vibrating module comprises a vibrating motor, and the upper heating module can be driven to quickly vibrate, so that bubbles in the capillary reaction tube are driven to the top of the capillary reaction tube, in the amplifying process, the bubbles produced in the convection PCR tube can be effectively eliminated, the situation that the heat convection is influenced bythe bubbles is avoided, the amplifying efficiency is reduced, and the influence on the fluorescence detection performance is avoided.

Description

technical field [0001] The invention relates to the fields of life medical detection and rapid diagnosis, in particular to a portable convective PCR amplification detection device with a vibration function. Background technique [0002] In traditional PCR amplification devices, amplification is carried out in a thermal cycle system, which requires repeated heating and cooling of amplification reagents to achieve thermal cycle and then achieve the purpose of PCR amplification. It means that a lot of time will be wasted on the temperature control of heating and cooling instead of nucleic acid amplification in the PCR amplification device adopting this kind of thermal cycle system, while the convection PCR technology only needs to carry out constant temperature control at the upper and lower ends of the amplification reagent, and the upper end It is around 55°C, and the lower end is around 95°C. The convection of the amplification reagent is realized through the temperature dif...

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Problems solved by technology

This detection method has relatively poor detection reproducibility because the PCR amplification has passed the logarithmic phase

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