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Microsatellite markers for detecting closed colony of Beagles and combination of microsatellite markers

A technology of microsatellite markers and Beagle dogs, applied in the field of microsatellite markers, can solve the problems of insufficient polymorphism, unsuitable detection and monitoring of genetic quality of closed group Beagle dogs, etc.

Active Publication Date: 2020-05-15
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(4) Carry out blood relationship identification and blood relationship control. In breeding, pedigree records are very important. They were first used in blood type and blood protein polymorphism analysis, but gave way to DNA fingerprinting technology due to insufficient polymorphism.
However, these microsatellite loci are completely unsuitable for the detection and monitoring of the genetic quality of closed beagle dogs

Method used

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  • Microsatellite markers for detecting closed colony of Beagles and combination of microsatellite markers
  • Microsatellite markers for detecting closed colony of Beagles and combination of microsatellite markers
  • Microsatellite markers for detecting closed colony of Beagles and combination of microsatellite markers

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1.1.1 Experimental animals

[0043] A total of 30 small beagle dogs of non-litmates and gender were randomly selected from Qingdao Bolong Experimental Animal Co., Ltd. 5mL of blood was collected from each individual vein into anticoagulant blood collection tubes, and stored in a 4°C refrigerator for later use.

[0044] Similarly, 6 medium-sized Beagle dogs without littermate and 6 large-sized Beagles without littermates purchased from Qingdao Bolong Experimental Animal Co., Ltd. were randomly selected. Coagulate blood collection tubes and store in a 4°C refrigerator for later use.

[0045] 1.1.2 Extraction and detection of genomic DNA from blood samples of Beagle dogs

[0046] Using a blood genomic DNA extraction kit (QIAamp DNA Blood Midi kit, Qiagen, Germany), the genomic DNA was extracted from the blood samples of the above-mentioned Beagle dogs according to the instructions. The concentration of the extracted genomic DNA was measured by a micro-ultraviolet spectr...

Embodiment 2

[0077] Purchased 30 closed groups of small Beagle dogs from 3 different domestic manufacturers in Xi'an Di Lepu, Beijing Masi and Jiangsu Zhaoshengyuan, and used the optimized combination of 20 microsatellite loci suitable for the genetic quality detection of closed group Beagle dogs It was evaluated for genetic quality verification.

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Abstract

The invention relates to microsatellite markers for detecting a closed colony of Beagles and combination of the microsatellite markers. The microsatellite markers comprise at least one of FH2776, PEZ1, FH3972, C08.618, C14.866, REN06C11, REN85N14, CPH17, PEZ3, FH2626, REN87O21, REN239K24, FH3082, AF8586 and PEZ2.

Description

technical field [0001] The invention relates to a microsatellite marker for detecting a closed group of beagle dogs, in particular to a combination of microsatellite markers for detecting a closed group of beagle dogs. Background technique [0002] The Beagle, also known as the Beagle Hound or Beagle, is native to England and is the smaller of the hounds. It was introduced to the United States in 1880 and is one of the internationally recognized breed dogs. It has the advantages of small size, docile personality, uniform response, good repeatability, well-developed brain and strong adaptability. It is very suitable for life science and medical research. It is an ideal experimental animal and an internationally recognized experimental dog. Beagle dogs are widely used in non-clinical drug research such as acute toxicity tests, and it has also been reported that beagle dogs are used to replicate animal models of oral and urinary system diseases. In addition, Beagles can be use...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杜小燕陈振文李长龙李银银路静蒋辉霍学云郭萌刘欣吕建祎
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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