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Method for detecting transmission medium of Sugarcane white leaf phytoplasma

A technology of transmission medium and determination method, which is applied in the field of determination of sugarcane white leaf disease phytoplasma transmission medium, and achieves the effect of objective determination results and meeting habitat conditions.

Active Publication Date: 2020-05-22
SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI
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  • Claims
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Problems solved by technology

Sugarcane white leaf disease is a new disease that has rapidly expanded in recent years. At present, there is no effective method for determining the phytoplasma transmission vector of sugarcane white leaf disease. In actual production, research and development of a method that can effectively detect the phytoplasma vector of sugarcane white leaf disease will help in-depth research and effective prevention and control of sugarcane white leaf disease, enhance disaster reduction and disaster prevention capabilities, and ensure the safe production of sugarcane and the high-quality development of the sucrose industry in China Has great significance and broad application prospects

Method used

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  • Method for detecting transmission medium of Sugarcane white leaf phytoplasma
  • Method for detecting transmission medium of Sugarcane white leaf phytoplasma
  • Method for detecting transmission medium of Sugarcane white leaf phytoplasma

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Embodiment 1

[0029] Embodiment 1 The method of the present invention measures the application of sugarcane white leaf disease phytoplasma transmission medium in sugarcane area (high incidence area of ​​white leaf disease) in Gengma County, Yunnan Province

[0030] Step Ⅰ measures the selection of field plots: in Gengma Dai and Wa Autonomous County, Lincang City, Yunnan Province (abbreviation: Gengma County) Gengma sugarcane area, this Gengma sugarcane area is a high-incidence area in the sugarcane white leaf disease area, and the field plots The crop rotation corn has been idle for more than 1 year, and the soil quality and fertility of the fields are relatively consistent, which are used as the measurement fields.

[0031] Step Ⅱ Determination of material selection: According to Li Wenfeng, Wang Xiaoyan, Huang Yingkun et al. The quarantine disease sugarcane white leaf disease caused by phytoplasma was found in Yunnan sugarcane area. The sugarcane varieties reported in Phytopathology, 2014,...

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Abstract

The invention relates to a method for detecting a transmission medium of Sugarcane white leaf phytoplasma. The method includes the steps that a piece of field in a sugarcane white leaf occurrence areaand with crop rotation for more than 1 year is selected; susceptible varieties are used for screening out phytoplasma infected with white leaf disease and phytoplasma not infected with the white leafdisease to be sugarcane species carried with virus and sugarcane species not carried with virus; the piece of field is divided into three field ditches, one of the field ditches is equipped with a transmission medium measurement field device in which the sugarcane species not carried with virus are planted, the sugarcane species carried with virus and the sugarcane species not carried with virusare planted in the other two field ditches separately, and morbidity situation is investigated; after newly planted sugarcane detection is completed, and biennial roots are cut and harvested; and according to the disease condition of the sugarcane species carried with virus, the sugarcane species not carried with virus, and newly planted and biennial root sugarcane inside and outside the device, asystematic analyzes to determine the transmission medium of Sugarcane white leaf phytoplasma. According to the method, a set of simple, convenient and systematic effective methods for determination of the transmission medium of Sugarcane white leaf phytoplasma is firstly established, theoretical guidance and scientific basis are provided for the effective prevention and control of Sugarcane whiteleaf, and the measurement results are objective, true and reliable.

Description

technical field [0001] The invention belongs to the technical field of phytoplasma transmission medium measurement, and in particular relates to a method for measuring the phytoplasma transmission medium of sugarcane white leaf disease. Background technique [0002] Sugarcane white leaf (SCWL) is the first important new disease of quarantine risk caused by phytoplasma (Sugarcane white leaf phytoplasma) on sugarcane in my country. The main symptoms are leaf whitening, increased tillers and dwarfing. The spread is very rapid and seriously threatens the production and development of sugarcane in my country. Sugarcane white leaf disease was first discovered in Lampang, northern Thailand in 1954, and has now widely occurred in Southeast Asian countries such as India, Pakistan, Sri Lanka, Japan, Laos, Myanmar, Vietnam, and the Philippines. SCWL is an extremely dangerous phytoplasmic disease that can cause devastating disasters to sugarcane. In Thailand, the incidence rate of SCWL...

Claims

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Application Information

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IPC IPC(8): A01G7/00C12Q1/70C12R1/94G01N33/00
CPCA01G7/00C12Q1/701G01N33/0098
Inventor 黄应昆张荣跃单红丽王晓燕李婕尹炯王长秘罗志明仓晓燕
Owner SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI
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