Methods and device for electrochemical luminescence detection

Abstract

The invention discloses a method and device for electrochemical luminescence detection, and the method comprises the steps: a sample processing step: preparing a detected target complex sample from ato-be-detected liquid sample, wherein the detected target complex sample comprises a complex comprising a marker, a detected target or a reference substance thereof, and magnetic particles; a detection step: mixing a luminescence agent with the detected target complex sample in a detection pool, and then carrying out electrochemical luminescence detection; wherein the sample treatment step is notcarried out in the detection cell. According to the technical scheme, the sample treatment step and the detection step are isolated from each other, so that the size of the detection instrument can begreatly reduced, and the requirements and difficulty for the service life of the detector electrode and flushing and / or cleaning of the electrode are reduced.

Description

technical field [0001] The present application relates to the field of biochemical detection, more specifically, to a method and device for electrochemiluminescence detection. Background technique [0002] At present, due to the advantages of wide detection range, high sensitivity, and relatively simple operation, the detection technology of electrochemiluminescence (ECL) has been widely used in biology, medicine, pharmacy, clinical, environmental, etc. , food, immunology and nucleic acid hybridization analysis and industrial analysis and other technical fields of detection work. In general, electrochemiluminescence detection technology can detect trace target analytes, such as microorganisms, drugs, hormones, viruses, antibodies, nucleic acids and other proteins, not only to make qualitative judgments on the existence of target analytes, but also to Accurate quantitative analysis of its concentration or content. [0003] Whether it is at the level of technology research a...

AI Technical Summary

Problems solved by technology

[0005] The main problem in the actual application of the ECL detection device based on the above-mentioned traditional technical solution is that the electrode is the core component of the detection cell with a complex structure and must be able to be used repeatedly thousands of times, so the detection accuracy is limited by the life of the electrode and the washing and / or Cleaning has a greater impact

If sufficient flushing and / or cleaning is not done, the detection accuracy will be reduced; and because the flushing and / or cleaning needs to be repeatedly flushed and cleaned, a large amount of waste liquid will be brought, and complex pipelines need to be configured system, resulting in complex instrument structure, large volume, high cost, and not suitable for rapid detection of small amount of samples

Therefore, although ECL has very good technological advancement, its current application is basically limited to the centralized detection of a large number of clinical samples such as laboratory departments in large hospitals.

Images