InDel marker related to dominant bright green characters of Chinese cabbage, primer of InDel marker, and application of InDel marker and primer

A technology of Chinese cabbage and traits, which is applied in the application field of InDel markers and crop breeding, and can solve problems such as the inability to develop functional markers and the failure to separate related functional genes

Active Publication Date: 2020-06-09
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

However, none of the above studies have been able to isolate related functional genes, let alone develop functional markers.

Method used

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  • InDel marker related to dominant bright green characters of Chinese cabbage, primer of InDel marker, and application of InDel marker and primer
  • InDel marker related to dominant bright green characters of Chinese cabbage, primer of InDel marker, and application of InDel marker and primer
  • InDel marker related to dominant bright green characters of Chinese cabbage, primer of InDel marker, and application of InDel marker and primer

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Embodiment 1

[0049] 1. Discovery of dominant bright green mutation in flower stems of Chinese cabbage and acquisition of mutant strains

[0050] In the inventor's previous research, a recombinant inbred line population RIL150 (Liu et al., 2019) was constructed using Chinese cabbage inbred lines 06-247 (bright green skin) and He102 (bright green skin) as parents (Liu et al., 2019). During this process, the inventors found that there was a segregation of bright green / waxy epidermis in the flower stem epidermis of F2 plants at the bolting stage, implying that the bright green epidermal flower stems of 06-247 and He102 were controlled by different alleles. In the process of constructing recombinant inbred lines using the method of single-grain transmission, 150 F3 individual plants were tracked and investigated to F7. During this process, it was found that the changes in the epidermal properties of these lines have the following three situations: The first situation is Most of the (75) strains...

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Abstract

The invention relates to an InDel marker related to dominant bright green characters of Chinese cabbage, a primer of the InDel marker, and an application of the InDel marker and the primer. A brassicacrop is a common crop with scapes as edible parts, and plants having a bright green epidermis trait are generally preferred by consumers. According to the invention, a mutant with a bright green character controlled by a dominant and single gene, and a bright green gene locus GL A09-2 and a unique candidate functional gene Bra024697 are determined through the bright green mutant gene controlled by the dominant gene through a bioinformatics technology. As a parent material, the mutant individuals can obviously reduce the workload of screening bright green character individuals, and have important significance for breeding of brassica crops.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to an InDel marker closely linked with the dominant bright green trait of Chinese cabbage, a primer sequence for detecting the molecular marker and its application in crop breeding. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily to be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Cabbage heart, cabbage stalk or cabbage moss all belong to different subspecies or varieties of Brassica in the family Brassicaceae. They all use young and delicious flower stems, flower buds and attached leaves after bolting as their main edible organs, of which the largest proportion is The edible part is the flower stem ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/04
CPCC12Q1/6895A01H1/04C12Q2600/156C12Q2600/13
Inventor 刘栓桃赵智中张志刚王立华王荣花李巧云王树彬
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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