Method for preparing in-situ and pulmonary metastasis tumor model through intra-tibial injection

Abstract

The invention discloses a method for preparing an in-situ and pulmonary metastasis tumor model through intra-tibial injection. A nude mouse is anesthetized by ketamine according to the amount of 100mg / kg, and an operation is performed after 5min. The method comprises the following steps: (1) enabling the left hind limb of the nude mouse to be bent, wherein the included angle of the tibia and thefemur is 45 degrees; (2) preparing a Hamilton LT type injection needle with a common 1ml syringe needle, enabling the needle to penetrate into the joint gap of the tibia and the femur, inserting the needle from the concave point of the joint fossa of the tibia, and slowly penetrating through the cancellous bone part of the tibia along the long axis direction of the tibia backbone to enter the marrow cavity, so that an obvious breakthrough feeling is achieved; (3) slowly injecting 10 microliters of human sarcoma cell suspension, wherein about 1 * 105 cells are injected; and (4) pulling out theneedle, and putting back the nude mouse into a cage after the nude mouse is revived. The success rate of the in-situ lung metastasis tumor prepared by the method is 100%, and the pathological characteristics of clinical osteosarcoma morbidity and lung metastasis can be simulated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing orthotopic and lung metastatic tumor models through intra-tibial injection. Background technique [0002] Osteosarcoma is a highly malignant bone-derived cancer characterized by osteoid production and is the most common primary malignant bone tumor and second cause of cancer-related death in children and adolescents (15-19 years). Osteosarcoma is the most common primary malignant tumor of the bone. The combination of surgery and chemotherapy has improved the survival of patients with non-metastatic osteosarcoma, and the 5-year survival rate has reached 75% to 77%. However, these therapeutic strategies have limited effect on patients with metastatic osteosarcoma and have not improved in the past 30 years. Specifically, the clinical prognosis of metastatic osteosarcoma is still poor, and patients with metastases survive 5 years after initial diagnosi...

AI Technical Summary

Problems solved by technology

[0006] Since subcutaneous transplanted tumors cannot reflect the clinical characteristics of osteosarcoma, it is necessary to use tumor animal models that can better reflect the clinical characteristics when studying the pathogenesis of osteosarcoma and evaluating the efficacy of anti-osteosarcoma. However, the tibia of mice has a small volume and is difficult to operate. Difficult and difficult to form tumors, it is hoped to provide a new and efficient method for preparing orthotopic and lung metastatic tumor models through intratibial injection

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