A method and special device for measuring differences in cold pain behavior of different mice

A special device and behavioral technology, which is applied in the field of measuring the difference in cold pain behavior of different mice, the method and its special device, can solve the problems of mouse injury, single function, and influence on the test, so as to increase the types and improve the test accuracy Effect

Active Publication Date: 2022-04-08
JINHUA VOCATIONAL TECH COLLEGE
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The acetone low temperature test is highly subjective, and human factors have a greater influence. Therefore, the more objective cold plate test is more widely used; and the tail wagging test is usually performed by researchers pressing the back of the mouse and letting its tail hang down in ice water. The existing mouse cold pain test device usually only has the function of one of the tests, the structure is simple, and the function is single. Among them, the tail wagging test is manually pressed by the mouse, which is easily injured by the mouse and affects test conducted

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method and special device for measuring differences in cold pain behavior of different mice
  • A method and special device for measuring differences in cold pain behavior of different mice
  • A method and special device for measuring differences in cold pain behavior of different mice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Please refer to the attached Figure 1~4 , the present invention provides a technical solution:

[0036] A method for measuring differences in cold pain behavior of different mice, comprising the following steps:

[0037] S1, get two groups of 6-week-old mice, 10 in each group, the first group is used for cold plate test, wherein two mice are not treated, as control group 1 and control group 2, the remaining 8 mice After intraperitoneal injection of anesthesia, it was fixed on the processing table, and the nerves of its right hind limb were ligated; the second group was used for the tail wagging test, in which two mice were injected with normal saline of equal weight as control group 1 and control group 2, and the rest 8 mice were anesthetized by intraperitoneal injection, fixed on the processing table, and ligated their tail nerves;

[0038] S2, the control group one of the cold plate test group is placed on the first container 7 on the fixed plate 2, and the other 9...

Embodiment 2

[0046] The method of this embodiment is basically the same as that of Example 1, the difference is that in S1, the hindlimbs and tails of the mice treated with nerve ligation in the two groups of mice were injected with equal weight and different gradient concentrations of analgesic drugs, and the control group 1 and The mice in control group 2 were injected with the same weight of normal saline, repeating S2-S4; used to test the analgesic effect of different concentrations of analgesic drugs.

Embodiment 3

[0048] The structure of this embodiment is basically the same as that of Embodiment 1, the difference is that, as Figure 5 and Figure 6 As shown, the moving block 6 and the fixed plate 2 are fixed with a limit plate 601 corresponding to the two sides of each cylinder 9, and the limit plate 601 is provided with a chute 602, and the two sides of the cylinder 9 are symmetrically fixed with sliders. 905, the sliding block 905 is slidably connected to the slide grooves 602 on both sides, so that the cylinder body 9 can be installed on the moving block 6 and the fixed plate 2 correspondingly, and can be easily removed after the test is finished.

[0049] In the chute 602, a block 603 is slidably connected, and a spring is provided between the inner end surface of the block 603 and the inner surface of the chute 602, so that after the slide blocks 905 on both sides of the cylindrical body 9 slide into the chute 602, two The spring on the side and the clamping block 603 clamp the c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for measuring the difference in cold pain behavior of different mice in the technical field of cold pain test and a special device thereof, including a water tank, a fixed plate and a thermometer are arranged in the water tank, an electronic stopwatch and a moving assembly are arranged on the fixed plate, And both sides of fixed plate are provided with first container and second container respectively, the present invention is by setting first container and second container on both sides of fixed plate, so that carry out cold plate test and tail wagging test simultaneously; By arranging moving block And screw and other structures, it is convenient to move the first container and the second container, so that the glass plate and the tail of the mouse are immersed in ice water, and the cold pain test is carried out; by setting the control group 1 and the control group 2, it is used for the control without nerve ligation at room temperature As well as the cold feeling without nerve ligation control, the type of control group is increased to improve the accuracy of the test; by setting up multiple experimental groups injected with different concentrations of analgesic drugs, in order to test the analgesic effect of drugs at different concentrations.

Description

technical field [0001] The invention relates to the technical field of cold pain test, in particular to a method for measuring the difference in cold pain behavior of different mice and a special device thereof. Background technique [0002] Neuropathic pain is pain caused by lesions or dysfunctions in the peripheral or central nervous system. Neuropathic pain has a wide range of causes, complex pathological mechanisms, and various symptoms. The pathophysiological characteristics are mainly manifested as hyperresponsiveness of pain sensation, such as hyperalgesia, hyperalgesia and spontaneous pain. Diabetes, cancer, autoimmune disease, constant stress, toxic chemicals, alcoholism, and the use of certain drugs can all contribute to the occurrence of these disorders. The currently proposed pathogenesis involves both peripheral and central neural pathways. [0003] Neuropathic pain is relatively common, with an incidence rate of about 1% in the population; it is a chronic pai...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A01K29/00A61K49/00A61D3/00
CPCA01K29/005A61K49/0008A61D3/00
Inventor 吴玲玲陈浩浩徐庆丁明星
Owner JINHUA VOCATIONAL TECH COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap