Micro-fluidic channel, micro-fluidic chip and method for processing cells

A microfluidic channel and microfluidic chip technology, applied in the field of biomedical engineering, achieves the effects of strong controllability, good portability and fast onset

Active Publication Date: 2020-07-24
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Therefore, there is currently no cell pre-excitation method that can meet the

Method used

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  • Micro-fluidic channel, micro-fluidic chip and method for processing cells
  • Micro-fluidic channel, micro-fluidic chip and method for processing cells
  • Micro-fluidic channel, micro-fluidic chip and method for processing cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 provides a microfluidic chip, and the provided microfluidic chip includes a substrate and a microfluidic channel formed on the substrate. Provided microfluidic channels such as figure 1 shown. The microfluidic channel can be divided into four parts: inlet 1, screening area 2, extrusion area 3 and outlet 4. The inlet 1 is directly connected to the screening area 2 through 5 bifurcated flow channels, and enters the extrusion area 3 after passing through the first connecting area 5 with a length of 2 mm and a width of 0.32 mm. The flow channel of the extrusion area 3 is divided into 64 extrusion The passages merge again and connect to the exit after passing through the second connecting area 6 with a length of 1.2 mm and a width of 0.32 mm. The inlet 1 and the outlet 4 are both circular with a diameter of 1 mm; the screening area 2 is arranged with rhombic columnar structures in sequence according to the direction of cell flow, and the rhombic side length of...

Embodiment 2

[0059] Embodiment 2 provides a kind of cell mechanical pre-excitation method, comprising:

[0060] 1. Prepare 3% (w / v) bovine serum albumin (BSA) solution and 0.01% (w / v) Pluronic F-127 solution in sterile phosphate buffered saline (PBS), and filter it with 0.22 μm membrane after completely dissolving filter sterilization;

[0061] 2. Use a polytetrafluoroethylene (PTFE) catheter with an outer diameter of 0.9 mm and an inner diameter of 0.5 mm to connect the syringe and the microfluidic chip (the microfluidic chip is the microfluidic chip prepared in Example 1), successively with 75% Alcohol, PBS and 3% (w / v) BSA solution degass and pre-rinse the microfluidic channel;

[0062] 3. After the mesenchymal stem cells were digested and centrifuged, they were resuspended in 0.01% (w / v) Pluronic F-127 solution to a density of 2-3×10 6 mL -1 The cell suspension was transferred to a 1mL sterile syringe;

[0063] 4. Load the syringe on the micro-injection pump, set the size of the 1 mL...

Embodiment 3

[0074] Example 3 Comparison of Cell Deformability

[0075] Referring to the design of a quantitative analyzer for cell deformability (Kenddra D.Nyberg, et al.Biophys.J., 2017, Quantitative Deformability Cytometry: Rapid, Calibrated Measurements of Cell Mechanical Properties), use AutoCAD to design microchannels for quantitative analysis of cell mechanical phenotypes (Such as Figure 6 As shown in a), this channel is to record the deformation of cells through weak transient extrusion, so as to characterize the deformability of cells (cells that have undergone this characterization are no longer suitable for use, that is, they are no longer used for culture or other applications) , the minimum line width at the narrow part has three specifications of 5, 7, and 9 μm, which can be applied to the comparison of deformability between various cells of different diameters. In this example, the mechanical phenotype quantitative analysis microfluidic channel with a line width of 5 μm wa...

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Abstract

The invention relates to a micro-fluidic channel, a micro-fluidic chip and a method for treating cells. The provided microfluidic channel comprises an inlet, a screening area, an extrusion area and anoutlet; the screening area is connected with the inlet, the screening area is provided with a barrier, and the barrier forms a preset gap channel in the screening area; the extrusion area is connected with the screening area through a first connection area, the extrusion area comprises at least one extrusion channel, and the width of the extrusion channel is set to be a preset width; and the outlet is connected with the extrusion area through a second connection area. The micro-fluidic chip provided by the invention comprises a substrate, and a microfluidic channel formed on the substrate. The provided method for treating cells comprises the step of treating the cells by utilizing the micro-fluidic channel or the micro-fluidic chip. Therefore, mechanical pre-excitation treatment of cellscan be rapidly realized, instantaneous remodeling of cytoskeletons and cell nucleuses of the cells is realized, and the mechanical properties of the cells are changed in a short time.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and specifically relates to microfluidic technology and cell pre-excitation treatment, in particular to a microfluidic channel, a microfluidic chip and a method for treating cells. Background technique [0002] Cell pre-excitation treatment refers to the pretreatment of cells with physical, chemical, biological and other factors to change and maintain the expected cell phenotype within a certain period of time, so as to serve the downstream research and application. A good cell pre-excitation method should meet the requirements of rapid onset of action, strong controllability, and high throughput. [0003] The traditional cell pre-excitation method is mainly based on the induction of biological factors, such as the use of IL-4 or IFN-γ and LPS to activate macrophages and regulate their typing, and the use of CD3 / CD28 magnetic beads and IL-2 to activate T cells to facilitate their development...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCC12M23/16C12M35/04
Inventor 杜亚楠陈宇杨
Owner TSINGHUA UNIV
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