A kind of Lubao No. 1 mutant strain and its application

A technology of bacterial Lubao and preservation number, applied in the field of "Lubao No. 1" mutant strains, can solve the problems of dodder pathogenicity and control effect reduction, and achieve high spore production ability and stable pathogenicity inheritance

Active Publication Date: 2022-03-29
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of "Lubao No. 1" reducing the pathogenicity and control effect of dodder, the present invention provides a mutant strain of "Lubao No. 1", which has a relatively improved pathogenicity and control effect

Method used

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  • A kind of Lubao No. 1 mutant strain and its application
  • A kind of Lubao No. 1 mutant strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Screening of highly pathogenic mutants

[0016] 1.1 Acquisition of positive mutants

[0017] The "Lubao No. 1" strain can be obtained through commercial purchase, such as the China Center for Microorganism Culture Collection (CMCC), and the platform number is Bio-17420. After the above-mentioned bacterial strains were activated, they were cultured on a 9cm PDA culture plate. After 10 days, the conidia on the surface of the colony were washed with 6 mL / dish of sterile water. After filtration, the concentration of spores in the filtrate was observed under a microscope through a hemocytometer, and diluted to The concentration of spores is 1×10 5 pc / mL, spare.

[0018] A single colony of Agrobacterium AGL-1 containing the freshly activated binary vector pBHt2 was picked, inoculated in 2 mL of LB liquid medium (kanamycin 50 μg / mL), and cultured at 220 rpm and 28 °C for 1 day. Take 250 μL of the shaken Agrobacterium solution and place it in 50 mL of MM medium (co...

Embodiment 2

[0027] Example 2 Control effect of highly pathogenic mutants on dodder

[0028] Refer to Example 1.2 to obtain the relevant method of highly pathogenic mutants to verify the control effect of the strain. After 10 days of inoculation, the remaining dodders in the same area after the control of the mutant and the wild type were picked, weighed, and compared with those of the clean water control group. Control effect (%) = (weight of dodder in control group - weight of dodder in inoculation group) / weight of dodder in control group. The inoculation groups were mutant and wild type, respectively.

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Abstract

The invention provides a dodder biocontrol bacteria Lubao-TB182 ( Colletotrichum acutatum ), the deposit number is CGMCC No.17472. Can be used to control dodder ( Cuscuta chinensis Lam.), Southern dodder ( Cuscuta australis R.Br.) or Japanese dodder ( Cuscuta japonica Choisy). The dodder biocontrol fungus provided by the invention is obtained by means of molecular biology, has high sporulation ability, has stable pathogenicity inheritance to dodder, and provides a new tool for biological control of dodder.

Description

technical field [0001] The invention relates to the field of biological control of weeds, in particular to a "Lubao No. 1" mutant strain. Background technique [0002] In recent years, my country has also achieved a series of achievements in the biological control of weeds, such as the initial use of Curvularia teff ( Culvularia eragrostidis ) Control crabgrass ( Digitaria sanguinalis ); using Alternaria pseudoalpacia ( Nimbya alternantherae ) to prevent and control lotus seedlings ( Alternantera phyloxeroides ) and using the neat Sclerotinia ( Sclerotium rolfsii ) to control Solidago canadensis ( Solidago canadensis ) have achieved certain results. [0003] Cuscuta ( Cuscuta spp) is a class of malignant parasitic plants belonging to Convolvulaceae or Cuscutaceae, and there are about 14 species in my country. Cuscuta grows rapidly, produces a large amount of seeds, has strong seed viability, and has a wide range of hosts. It has serious and long-lasting damage t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01P13/00C12R1/645
CPCC12R2001/645C12N1/145
Inventor 李健李美高兴祥房锋
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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