Single-spore propagation method for Gigaspora rosea mycorrhiza fungi

Abstract

The invention provides a single-spore propagation method for Gigaspora rosea mycorrhiza fungi. The method includes the following steps: inoculating the Gigaspora rosea mycorrhiza fungi single spores into a sucrose-free M culture medium for culture to obtain germinated single spores; inoculating the germinated single spores into a carrot hairy root-M culture medium system, culturing and screening to obtain a high-spore-yield Gigaspora rosea mycorrhiza fungi single-spore propagation system; re-inoculating the single-spore propagation system into a carrot hairy root-M culture medium system, and expanding culture to obtain a solid culture; and mixing and incubating the solid culture with a gel dissolving solution, leaching and sieving to obtain a propagated Gigaspora rosea mycorrhiza fungi spore suspension. The method can quickly obtain a large number of progeny spores from a single spore, has good expanded spore germination and good host infection activity, and can provide ideal inoculantmaterials for basic research and application research such as arbuscular mycorrhizal fungi genetics and molecular biology.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, and in particular relates to a single-spore propagation method of the mycorrhizal fungus Megacystis roseum. Background technique [0002] Arbuscular mycorrhizal fungi (AMF) can significantly promote the uptake of soil phosphorus by host plants, so they have the potential to be used as biofertilizers. In addition, research on the interaction between arbuscular mycorrhizal fungi and host plants is still one of the current biological research hotspots. The current basic and applied research on arbuscular mycorrhizal fungi mainly focuses on a few species of Glomerales, which is mainly related to the ease of cultivation and propagation of species in this order. In fact, there are many species of arbuscular mycorrhizal fungi, and there may be significant physiological and ecological differences between each branch. Therefore, its basic and related applied research needs to include more s...

AI Technical Summary

Problems solved by technology

The traditional potted propagation method involves the process of seedling raising, inoculation and later plant growth management, and the operation is relatively complicated

Importantly, the entire cultivation process after inoculation cannot be monitored in real time, and the mycorrhizal infection status of the inoculated plants cannot be known until the final sample is collected

When the single-spore inoculation method is adopted and there are many inoculated plants, but the proportion of plants actually infected is not high, the potted method will undoubtedly increase a lot of unnecessary labor costs

In addition, the traditional potting method mostly adopts an open or semi-open greenhouse environment, and the proliferating bacteria are easily polluted by other miscellaneous bacteria.

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