Application of protein molecular marker in fish germ cell development research

A protein molecule and germ cell technology, applied in the application field of protein molecular markers in the research of fish germ cell development

Active Publication Date: 2020-08-04
HUNAN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide the application of a protein molecular marker in the research of fish germ cel...

Method used

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  • Application of protein molecular marker in fish germ cell development research
  • Application of protein molecular marker in fish germ cell development research
  • Application of protein molecular marker in fish germ cell development research

Examples

Experimental program
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Embodiment 1

[0055] Example 1 Protein histochemical detection of fish in different developmental states

[0056] In this example, the protein molecule Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) purchased from Abcam Company, which will be abbreviated as RNA Pol II CTD (S5) later, was used for protein histochemical detection on testis tissues of fish in different developmental states.

[0057] The research objects of this embodiment adopt the original parent red crucian carp and hybrid crucian carp F 1 and allotetraploid crucian carp, among which, the original parent red crucian carp is a sexually fertile 1-year-old natural species red crucian carp, and the allotetraploid crucian carp is a sexually fertile 1-year-old artificially selected hybrid heterologous fourfold Body crucian carp, hybrid crucian carp F 1 A 1-year-old artificial hybrid carp carp F with fertile potential but not overcoming reproductive barriers was selected 1 , the specific production process of hybrid cruci...

Embodiment 2

[0079] Example 2 Immunofluorescence localization analysis of fish in different developmental states

[0080]In this example, the protein molecule Anti-RNA polymerase II CTD repeatYSPTSPS (phospho S5) purchased from Abcam Company, which will be referred to as RNA Pol II CTD (S5) later, was used to perform immunofluorescence localization analysis on germ cell chromosomes of different fish.

[0081] The research objects of this embodiment adopt the original parent red crucian carp and hybrid crucian carp F 1 and allotetraploid crucian carp, among which, the original parent red crucian carp is a sexually fertile 1-year-old natural species red crucian carp, and the allotetraploid crucian carp is a sexually fertile 1-year-old artificially selected hybrid heterologous fourfold Body crucian carp, hybrid crucian carp F 1 A 1-year-old artificial hybrid carp carp F with fertile potential but not overcoming reproductive barriers was selected 1 , The specific production process of hybrid...

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Abstract

The invention provides an application of a protein molecular marker in fish germ cell development research. The protein molecule is Anti-RNA polymerase II CTD repeat YSPTSPS (Phospho S5), and the application comprises the step of carrying out immunofluorescence localization analysis on fishes in different development states by adopting the protein molecule as a marker molecule. The immune probe isapplied to fish germ cell development research for the first time, cell facies characteristics of different fish germ cell developments are comprehensively analyzed, an important molecular marker isprovided for judging the development state of the fish germ cells, and a new development function detection strategy is provided for fish genetic breeding.

Description

technical field [0001] The invention belongs to the technical field of fish cell marking methods, and in particular relates to the application of protein molecular markers in the research of fish germ cell development. Background technique [0002] Functional molecular markers of germ cell development are helpful to study the reproductive mechanism and fertility of experimental subjects. They are widely studied in mammals and clinical medicine. Chinese scholars have systematically described the gene expression regulatory network in the process of human spermatogenesis from the single-cell level. and cell fate transition pathways, functional molecular markers of human germ cell development have been widely used. Germ cell marker genes that have been identified and analyzed in fish are also increasing, including vasa, nanos, dnd, dazl, sdf1 and cxcr, etc., but their expression is mostly concentrated in primordial germ cells (PGCs). During the development of male germ cells in...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N21/64
CPCG01N21/6486G01N33/53
Inventor 张纯刘少军朱书润胡方舟杨聪慧王石汤陈宸李琪贺旺超
Owner HUNAN NORMAL UNIVERSITY
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