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PCR sampling detection device

A technology of detection device and detection box, which is applied in the fields of inoculation ovulation diagnosis, medical science, fluorescence/phosphorescence, etc. It can solve the problems of threatening the life safety of medical staff and inspection personnel, unable to realize popularization of the field, and many safety guarantee links. Achieve the effect of simple piercing mechanism, avoid manual extrusion, and avoid inaccurate detection

Pending Publication Date: 2020-08-07
朱建国
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The source of infection used for infectious diseases and other pathogen detection methods that may pose a threat to medical staff need to rely on professional-level laboratories and testing equipment, involving a series of processes such as pathogen collection, storage, transfer, processing and testing, and require professional The process is time-consuming and labor-intensive, and there are too many safety assurance links, which are prone to pathogen leakage and other pollution problems, threatening the lives of medical staff and inspection personnel; traditional PCR instruments are bulky and require professionals to use The pre-processing and operation of samples cannot be achieved in the field of popularization

Method used

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Embodiment 1

[0035] Embodiment 1: as Figure 1-Figure 9 As shown, a PCR sampling detection device includes a detection box 1, a PCR reaction prefabricated tube 2, a fluorescent probe 3, a heating device 4 and a sampler 9. The upper end of the detection box 1 is open, and a support ring 5 is arranged in the upper end, and the PCR reaction prefabricated The tube 2 is detachably fixedly connected to the support ring 5, and the middle part protrudes from the through hole in the center of the support ring 5. The fluorescent probe 3 is fixedly connected to the lower end surface of the support ring 5 and faces the downward convex part of the PCR reaction prefabricated tube 2. A heating device 4 is installed under the middle part of the reaction prefabricated tube 2, and the heating device 4 is fixedly connected in the detection box 1. The sampler 9 can be covered on the upper end of the detection box and can squeeze the liquid on the sampling test paper into the PCR reaction prefabricated tube. 1...

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Abstract

The invention discloses a PCR sampling detection device. The device comprises a detection box, a PCR reaction prefabricated tube, a fluorescent probe, a heating device and a sampler. The upper end ofdetection box is opened, and a support ring is arranged in the upper end; the PCR reaction prefabricated tube is fixedly connected to the supporting ring, and the middle part of the PCR reaction prefabricated tube downwards protrudes out of a through hole in the center of the supporting ring; the fluorescent probe is fixedly connected to the lower end face of the supporting ring and right faces the downward-protruding part of the PCR reaction prefabricated tube; the heating device is installed below the middle of the PCR reaction prefabricated tube and fixedly connected into the detection box,and the sampler can cover the upper end of the detection box and squeeze liquid on the sampling test paper into the PCR reaction prefabricated tube. After the detection device is covered with the sampled sampler, a sample on the sampling test paper head of the sampler is squeezed into the PCR reaction prefabricated tube and subjected to a heating reaction, the fluorescent probe is adopted for detection, PCR rapid sampling detection is achieved, the structure is greatly simplified, carrying is convenient, the cost is greatly reduced, and constant-temperature detection can be achieved.

Description

technical field [0001] The invention relates to a PCR sampling and detection integrated device, belonging to the technical field of PCR detection equipment. Background technique [0002] The source of infection used for infectious diseases and other pathogen detection methods that may pose a threat to medical staff need to rely on professional-level laboratories and testing equipment, involving a series of processes such as pathogen collection, storage, transfer, processing and testing, and require professional The process is time-consuming and labor-intensive, and there are too many safety assurance links, which are prone to pathogen leakage and other pollution problems, threatening the lives of medical staff and inspection personnel; traditional PCR instruments are bulky and require professionals to use Pre-processing and operation of samples cannot be achieved in the field of popularization. Contents of the invention [0003] The technical problem to be solved by the p...

Claims

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Application Information

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IPC IPC(8): G01N21/64A61B10/00A61B10/02
CPCA61B10/0051A61B10/02G01N21/64
Inventor 陈伟明刘章成江克华杨胜邦袁东波宋具昆朱国华焦珂陈潇月苏浩张畅罗福张伟陈卫红胡彬张帆刘恒
Owner 朱建国
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