The invention discloses an extraction method of total
saponin of Radix Ilicis Asprellae and a quality detection method thereof, and the extraction method comprises the following steps: taking a medicinal material of Radix Ilicis Asprellae root or stem, adding 4-12 times volume of 60%
ethanol, extracting for 1.5-2h, adding 2-10 times volume of 60%
ethanol, extracting for 1.0-1.5h, filtering and merging filtrate; concentrating under reduced pressure until no
alcohol taste exists, adding water, diluting
crude drug with the loading concentration of 0.1-0.5g / ml, and loading a pre-treated AB-8
macroporous resin column; washing the resin column to be cleared by water, and eluting by 60-80% of
ethanol; and collecting eluent of 60-80% ethanol and concentrating under reduced pressure,
drying to obtain the total
saponin of Radix Ilicis Asprellae. The quality detection method comprises the steps of TLC chromatograph identification and
content determination of ilexoside X XIX in the total
saponin of Radix Ilicis Asprellae. By using the refined technology of the invention, the retention rates of the total saponin of Radix Ilicis Asprellae and f ilexoside X XIX are improved greatly, and the obtained extract has higher purity; the obtained total saponin of Radix Ilicis Asprellae has the functions of
inflammation resistance, analgesis, koortswerend, antivirus and the like; by using the characteristic saponin ilexoside X XIX as a
reference sample, the accuracy is higher and the result has high reliability; and the quality detection method of the total saponin of Radix Ilicis Asprellae extract can provide a reference for
quality control of the total saponin of Radix Ilicis Asprellae extract.