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Metal organic framework composite nanomaterial with targeting property and pH responsiveness as well as preparation method and application of metal organic frame composite nanomaterial

A metal-organic framework and composite nanomaterial technology, applied in the field of material science, can solve the problems of increasing T cell anti-tumor, lack of targeting ability, drug leakage, etc., achieving high drug loading rate, enhancing the content and effect of anti-tumor substances Good results

Inactive Publication Date: 2020-09-11
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some shortcomings in the simple application of MOF as a drug carrier: it does not have targeting ability, and it is easy to degrade in water and cause drug leakage.
So far, there has been no report on the use of materials to increase functionally active substances in T cells to complete anti-tumor

Method used

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  • Metal organic framework composite nanomaterial with targeting property and pH responsiveness as well as preparation method and application of metal organic frame composite nanomaterial
  • Metal organic framework composite nanomaterial with targeting property and pH responsiveness as well as preparation method and application of metal organic frame composite nanomaterial
  • Metal organic framework composite nanomaterial with targeting property and pH responsiveness as well as preparation method and application of metal organic frame composite nanomaterial

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1: CD63-apt@CaCO3@ZIF-8@BSA

[0064] (1) Preparation of ZIF-8-encapsulated bovine serum albumin (ZIF-8@BSA):

[0065] First, 4 mg of bovine serum albumin (BSA) was prepared into an aqueous solution with 8 mL of distilled water, 8 mg of polyvinylpyrrolidone (PVP) was added to the aqueous solution of bovine serum albumin, and stirred gently for 30 seconds; then 4 mg of L-cysteine (L-cys) was added to the above reaction system, and stirred gently for 30 seconds after ultrasonic dispersion; finally, 8 mL of an aqueous solution containing 105 mg of 2-methylimidazole (2-mIM) and an aqueous solution of 73 mg of zinc acetate dihydrate were added to the system 8mL. After standing at room temperature for 6 hours, the core particles were collected by washing with distilled water several times and centrifuged.

[0066] (2) Calcium carbonate-wrapped metal-organic framework material CaCO 3 Preparation of @ZIF-8@BSA:

[0067] First, 7.5mL of cyclohexane, 1.77mL of Triton X...

Embodiment 2

[0070] Example 2: ROX-CD63-apt@CaCO3@ZIF-8@BSA

[0071] (1) Preparation of ZIF-8-encapsulated bovine serum albumin (ZIF-8@BSA):

[0072] First, 4 mg of bovine serum albumin (BSA) was prepared into an aqueous solution with 8 mL of distilled water, 8 mg of polyvinylpyrrolidone (PVP) was added to the aqueous solution of bovine serum albumin, and stirred gently for 30 seconds; then 4 mg of L-cysteine (L-cys) was added to the above reaction system, and stirred gently for 30 seconds after ultrasonic dispersion; finally, 8 mL of an aqueous solution containing 105 mg of 2-methylimidazole (2-mIM) and an aqueous solution of 73 mg of zinc acetate dihydrate were added to the system 8mL. After standing at room temperature for 6 hours, the core particles were collected by washing with distilled water several times and centrifuged.

[0073] (2) Calcium carbonate-wrapped metal-organic framework material CaCO 3 Preparation of @ZIF-8@BSA:

[0074] First, 7.5mL of cyclohexane, 1.77mL of Trit...

Embodiment 3

[0077] Example 3: CD63-apt@CaCO3@ZIF-8@BSA-TRITC

[0078] (1) Preparation of ZIF-8-encapsulated fluorescent bovine serum albumin (ZIF-8@BSA-TRITC):

[0079]First, 4 mg of bovine serum albumin (BSA) was prepared into an aqueous solution with 8 mL of distilled water, 8 mg of polyvinylpyrrolidone (PVP) was added to the aqueous solution of bovine serum albumin, and stirred gently for 30 seconds; then 4 mg of L-cysteine (L-cys) was added to the above reaction system, and stirred gently for 30 seconds after ultrasonic dispersion; finally, 8 mL of an aqueous solution containing 105 mg of 2-methylimidazole (2-mIM) and an aqueous solution of 73 mg of zinc acetate dihydrate were added to the system 8mL. After standing at room temperature in the dark for 6 hours, the core particles were collected by washing with distilled water several times and centrifuged.

[0080] (2) Calcium carbonate-wrapped metal-organic framework material CaCO 3 Preparation of @ZIF-8@BSA-TRITC:

[0081] First,...

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Abstract

The invention discloses a metal organic framework composite nanomaterial with targeting property and pH responsiveness as well as a preparation method and application of the metal organic framework composite nanomaterial. The metal organic framework composite nanomaterial is prepared from protein-loaded ZIF-8 metal organic framework nano-particles, calcium carbonate structures wrapping the ZIF-8 nano-particles and aptamers fixed to calcium carbonate structures. The preparation method is simple and convenient to operate, and damage to protein activity is avoided. The composite nano material provided by the invention has good protein loading capacity and targeting performance, and can gradually release contents in a low pH environment. The metal organic framework composite nanomaterial disclosed by the invention can also target T cells to increase the number of intracellular anti-tumor active substances, and can be developed into a novel anti-tumor biological therapy.

Description

technical field [0001] The invention belongs to the technical field of material science, and in particular relates to a metal-organic framework composite nanomaterial with targeting and pH responsiveness, a preparation method and application thereof. Background technique [0002] Metal-organic frameworks (MOFs) are inorganic-organic hybrids formed by the regular coordination of metal ions and organic ligands. They have important applications in hydrogen storage, gas adsorption and separation, sensors, and catalytic reactions. Due to its fine chemical composition, uniform size and controllable porous structure, an advanced application of MOF in the biological field is to use MOF as a protective network to immobilize functional biomacromolecules, such as nucleic acids and proteins. This immobilization provides effective control over the reaction process and enhances the stability of biomacromolecules under storage and handling conditions, providing a promising platform for dru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/42A61K47/22A61K47/02A61K39/00A61P35/00
CPCA61K9/5115A61K9/5123A61K9/5169A61K39/0011A61P35/00
Inventor 张玉峰赵钦宫子健
Owner WUHAN UNIV
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