Extraction method of total saponin of Radix Ilicis Asprellae and quality detection method thereof
A quality detection method and an extraction method technology, applied in the quality detection field of Gangmei total saponins, can solve the problems such as the blank quality detection method of Gangmei total saponins
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Embodiment 1
[0043] Example 1 The extraction process of the total saponins of Prunus officinalis
[0044] 1. Orthogonal experimental design
[0045] The retention rates of total saponins and ilexoside X X IX were used as indicators respectively, and an orthogonal experiment design with four factors and three levels was adopted. Weigh 20 g of Gangmei medicinal material, carry out the experiment according to the orthogonal design in Table 1, combine the filtrates, and measure the volume.
[0046] Table 1 Factor level table of orthogonal experiment for the extraction process of Gangmei medicinal materials
[0047]
[0048] 2. Preparation of the test solution
[0049] 2.1 Preparation of total saponins test solution
[0050] Accurately measure the appropriate amount of medicinal liquid under each orthogonal test item, concentrate under reduced pressure until there is no alcohol smell, dilute with water until the loading concentration is 0.2mg of crude drug / ml, accurately measure ...
Embodiment 2
[0073] Example 2 AB-8 Macroporous Adsorption Resin Refining Process of Gangmei Total Saponins Extract
[0074] 1. Investigation of sample concentration
[0075] 1.1 Preparation of Gangmei Extract and Determination of Gangmei Total Saponins in Extract
[0076] Get 905g of Gangmei medicinal material coarse powder (batch number 20080401 of Chinese medicine beverage factory of Guangzhou medicinal material company), add 8 times of 60% ethanol, heat and reflux for 1.5 hours, filter, add 6 times of 60% ethanol for filter residue, heat and reflux for 1 hour, filter, combine the filtrates, and measure the volume to obtain 11020ml of extract, which is set aside. The total saponin content was determined.
[0077] 1.2 Investigation of the retention rate of total saponins at different loading concentrations (centrifugation, static adsorption)
[0078] Measure 608.8ml of Gangmei extract (equivalent to containing 50g of crude drug), recover ethanol under reduced pressure, concentrate to 1...
Embodiment 3
[0133] Example 3 Verification of the purification process of Gangmei total saponins extract AB-8 macroporous resin
[0134] 1. Preparation of Gangmei Extract and Determination of Gangmei Total Saponins and Ilexoside X X IX in the Extract
[0135] According to the proposed conditions, get 250g of Gangmei medicinal material coarse powder (Chinese medicine beverage factory of Guangzhou medicinal material company, batch number: 20080401), add 8 times of 60% ethanol, heat and reflux for 1.5 hours, filter, and add 6 times of 60% ethanol to the filter residue. % ethanol, heated to reflux for 1 hour, filtered, combined the filtrates, and measured the volume to obtain 3016ml of extract, which was set aside. Determination of the content of total saponins and ilexosideX X IX of Gangmei.
[0136] 2. Verification of the refining process of Gangmei AB-8 macroporous adsorption resin
[0137] According to the maximum loading amount of Gangmei medicinal liquid macroporous resin determined in...
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