16 results about "Apoptotic gene" patented technology

The invention relates to application of WDR74 and / or ALYREF as molecular targets in esophageal squamous cell carcinoma diagnosis and treatment, and belongs to the technical field of biological medicine. Aiming at the problem that the esophageal squamous cell carcinoma lacks an effective targeted treatment means, the invention discovers that the expression quantity of WDR74 in tumor tissues is obviously higher than that in para-carcinoma tissues, and the high expression of WDR74 prompts poor prognosis of a patient, and reveals that WDR74 protein and ALYREF protein have specific binding, and the mRNA stability of EGFR is enhanced through ALYREF-mediated m5C RNA epigenetic modification, so that STAT3 phosphorylation is activated, and the treatment effect of the esophageal squamous cell carcinoma is enhanced. Further, the STAT3 is combined with the promoter region of the apoptosis-inhibiting gene MCL1, and finally cell apoptosis is inhibited and tumor formation is promoted. The invention provides a new molecular target and a solution for developing a WDR74 and ALYREF targeting medicine for treating esophageal squamous cell carcinoma and related diagnosis and prognosis evaluation products.

The invention discloses an application of SB216763 in improving the in-vitro proliferation capacity of porcine spermatogonium. Therefore, the SB216763 can be used as an additive of the porcine spermatogonial cell in-vitro culture medium, and the porcine spermatogonial cell in-vitro culture medium combined with various growth factors is prepared. Researches show that clone groups of the porcine spermatogonium cultured by the culture medium additionally added with the SB216763 are increased, the number of the clone groups is remarkably increased, the expression quantity of genes related to proliferation is remarkably increased, apoptosis genes are remarkably reduced, and the expression of genes related to reproductive specificity and pluripotent maintenance is also increased, so that the in-vitro proliferation and long-term maintenance of the porcine spermatogonium are improved, and the porcine spermatogonium can be used for preparing a novel culture medium for the porcine spermatogonium. Therefore, the in-vitro culture level of the PSCs is systematically improved. Therefore, the invention provides a reliable basis for establishing a long-term, stable and efficient in-vitro culture system of the porcine spermatogonium, and provides a stable and efficient cell model for gene editing of the porcine spermatogonium, preparation of transgenic animals and research on reproductive diseases; the method also has potential application value in the aspects of genetic improvement of pig breeds or preservation of male germplasm resources.

The invention belongs to the technical field of biology, and particularly relates to application of creatine in relieving heat stress injury of bovine mammary epithelial cells. It is innovatively found for the first time that creatine can improve the activity of bovine mammary epithelial cells suffering from heat stress, reduce the apoptosis amount, caused by heat stress, of the bovine mammary epithelial cells, inhibit expression of pro-apoptosis genes and promote expression of anti-apoptosis genes, and the method provides a new method for relieving or treating bovine heat stress injury, and has the advantages that the creatine is used for treating bovine heat stress injury; the composition can also be used as a protective agent for cattle to resist heat stress.

The application relates to a circular triplex-forming oligonucleotide, a preparation method and application in preparation of tumor-targeting drugs. The circular triplex-forming oligonucleotide (Cir-TFO) is composed of two oligonucleotide chains A and B, each of which comprises a first complementary sequence, a first connecting sequence, a target sequence, a second connecting sequence and a second complementary sequence from the 5' end to the 3' end. The first complementary sequences of the two oligonucleotide chains A and B are reversely and complementarily connected, and the second complementary sequences are reversely and complementarily connected to form a closed ring structure. The target sequence is a TFO sequence capable of forming a triplex structure with double-stranded DNA in the promoter region of a target gene through Hoogsteen hydrogen bonds, and the target gene is selected from a proto-oncogene, an anti-apoptotic gene or a tumor metabolism-related gene.

The invention discloses a plant lactobacillus and application thereof in relieving aflatoxin B1 induced liver injury, and relates to the technical field of microorganisms. The lactobacillus plantarum disclosed by the invention is lactobacillus plantarum TY-1, and the preservation number of the lactobacillus plantarum TY-1 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M 20251682. The lactobacillus plantarum TY-1 disclosed by the invention has the advantages that the lactobacillus plantarum TY-1 is a lactobacillus plantarum TY-1, and the preservation number of the lactobacillus plantarum TY-1 in the China Center for Type Culture Collection (CCTCC); the bacterial strain has bacteriostatic activity and better oxidation resistance, and can effectively reduce the level of proinflammatory factors in serum, down-regulate the relative expression of pro-apoptosis genes and reduce the apoptosis of liver cells, thereby effectively relieving the damage to the liver caused by exposure to aflatoxin B1.

The application discloses application of TESC as an Alzheimer's disease prevention and treatment target and relates to the field of biological medicines. A TESC overexpression (TESC-OE) hippocampal neuron cell line is constructed through lentivirus transfection, and the result shows that TESC-OE has the ability to resist A beta-induced neuron apoptosis and synapse damage. A TESC overexpression model in the hippocampus of a mouse is constructed through stereotactic injection of an adeno-associated virus, and the result shows that the hippocampal gray matter volume in the brain of the TESC-OE mouse is significantly larger than that of a wild type mouse. A beta stereotactic modeling is performed on the basis of TESC overexpression in the hippocampus, and the result shows that the TESC-OE mouse can significantly resist A beta-induced hippocampal atrophy, learning and memory dysfunction and impaired synaptic plasticity. Immunoblotting analysis reveals the molecular mechanism that TESC can increase the expression of anti-apoptotic genes in the hippocampus and reduce the expression of pro-apoptotic genes to exert a neuroprotective effect.

This invention relates to the field of biomedical technology, and in particular to the application of hD1R protein in the preparation of drugs for the treatment of AML. Addressing the current lack of systematic and in-depth research on whether hD1R protein can directly act on AML cells and whether it has a synergistic effect with existing standard chemotherapy drugs (such as anthracyclines), this invention, through a series of in vivo and in vitro experiments and the construction of a NOD / SCID mouse transplantation model, confirms that hD1R protein alone can effectively inhibit AML cell proliferation, induce apoptosis, inhibit AML cell colony formation, and downregulate the expression of the key anti-apoptotic gene Bcl2 in AML cells. It also confirms that hD1R treatment can significantly prolong the survival of model mice. Furthermore, by setting up a Dox+hD1R group (combined experimental group), it is confirmed that when hD1R protein is used in combination with anthracycline chemotherapy drugs, it exhibits significant synergistic effects in inhibiting AML cell proliferation, promoting AML cell apoptosis, downregulating the expression of the anti-apoptotic gene Bcl2 in AML cells, and inhibiting AML cell colony formation.

The application belongs to the technical field of biological medicine, and particularly relates to application of an imidazoquinazoline small-molecule compound or a pharmaceutically acceptable salt thereof shown in formula (I) in preparation of a drug for treating KRAS mutant gastrointestinal tumors, wherein R is as described in the claims and the specification. The imidazoquinazoline compound can selectively inhibit proliferation, migration and invasion of KRAS mutant gastrointestinal tumor cells, and induce cell cycle arrest and apoptosis. Specifically, the compound exerts an anti-tumor effect by inhibiting the binding of p-ERK2 and p53, restoring p53 transcriptional activity, and up-regulating the expression of a pro-apoptotic gene PUMA downstream of p53. The mechanism of action is different from that of traditional KRAS or MEK inhibitors, and provides a new treatment option for KRAS mutant gastrointestinal tumor patients.

The invention discloses a composite single-cell protein feed for improving anti-infection immunity of micropterus salmoides and application of the composite single-cell protein feed, and belongs to the technical field of feed preparation. The feed comprises composite single-cell protein for replacing 40-100% of fish meal, and the composite single-cell protein comprises chlorella and clostridium ethanol protein in a mass ratio of 1: (4-5). The compound single-cell protein is adopted to replace part of fish meal in the feed, so that the cost of the feed can be remarkably reduced, the anti-infection immunity of the micropterus salmoides to aeromonas hydrophila can be improved, expression down-regulation of a pro-apoptosis gene casp8 and an antigen presentation related gene mhci in the micropterus salmoides can be promoted, and the apoptosis resistance of the micropterus salmoides can be improved. The expression up-regulation of the anti-apoptosis gene bcl2l1 and the anti-ferroptosis gene gpx4a is promoted. In addition, the liver metabolism function of the micropterus salmoides can be improved, and lipid metabolism is promoted.

The invention relates to the technical field of biology, and discloses an enhanced packaging cell for preparing lentiviruses of CAR and other toxic genes and application of the enhanced packaging cell, the enhanced packaging cell enables key genes in an endogenous apoptosis pathway to be completely inactivated through gene editing so as to improve the resistance of the packaging cell to the toxic genes including the CAR, the key genes in the endogenous apoptosis pathway comprise at least one pro-apoptosis gene of BAX and BAK or BIM, PUMA, NOXA, BAD and BID. When the lentiviral vector carrying CAR or other toxic genes such as PUMA is packaged, the lentiviral vector shows strong adaptability, the virus titer which is remarkably higher than that of a traditional packaging cell is obtained, the cell toxin production time can be remarkably prolonged, and the lentiviral vector can be used for preparing the lentiviral vector. And a more effective tool can be provided for solving the problem of high cost of large-batch preparation of viruses in clinical research of CAR-T and lentiviruses with certain toxic genes.

This invention provides a method for preparing and applying a tea polyphenol-crosslinked decellularized diaphragmatic tendon. The tendon is formed by decellularizing porcine diaphragmatic tendon with 1% TNBP and then crosslinking it with tea polyphenols. The tea polyphenol-crosslinked decellularized diaphragmatic tendon can downregulate the expression of apoptosis and inflammatory genes and upregulate the expression of antioxidant genes, exhibiting strong antioxidant properties, mechanical properties, anti-enzymatic properties, and low immunogenicity. It can enhance the antioxidant capacity of cardiomyocytes, promote myocardial tissue vascularization and inhibit fibrosis, inhibit ventricular dilation, improve fibrosis, and promote angiogenesis. It can be used in the preparation of patches for treating myocardial infarction. The tea polyphenol-crosslinked decellularized diaphragmatic tendon provided by this invention has promising applications in the treatment of myocardial infarction.

The invention provides application of amentotaxus biflavone in preparation of a medicine for treating bladder cancer, and provides application of amentotaxus biflavone in preparation of a medicine for treating bladder cancer, which has the advantages that by increasing the concentration of amentotaxus biflavone, the number of bladder cancer cells is gradually reduced, the cells are shrunk, nuclear shrinkage of different degrees is caused, the activity, proliferation, invasion and migration of bladder cancer T24 cells and bladder cancer 5637 cells are inhibited, and the bladder cancer is treated. The PI3K / AKT / NF-kappa B pathway promotes the expression of a pro-apoptosis gene Bax in bladder cancer cells, inhibits the expression of an apoptosis inhibitor gene Bcl-2, inhibits the proliferation of the bladder cancer cells by inducing G1 phase retardation of the bladder cancer cells, induces and reduces the expression of PI3K, AKT and NF-kappa B proteins in cell nucleuses through the PI3K / AKT / NF-kappa B pathway, and induces the apoptosis of the bladder cancer cells. The invention belongs to the field of medicine.

The invention discloses an anti-apoptosis CHO cell strain as well as a construction method and application thereof. The invention belongs to the technical field of bioengineering. According to the invention, three key apoptosis genes, namely BAK, BAX and Casp3 (Caspase-3), in a CHO-K1 cell line are knocked out at the same time through a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) gene editing technology, so that a CHO cell 3 gene knockout cell line is established, and the CHO cell 3 gene knockout cell line is named as a CHO-MT cell line. Experiments prove that the CHO-MT cell shows remarkable anti-apoptosis ability, the life cycle of the cell is prolonged, the overall activity of the cell is greatly improved, and the expression level of foreign protein is remarkably improved. The successful construction of the CHO-MT cell line provides an efficient and stable host cell for the biopharmacy industry, and is especially suitable for producing high-quality therapeutic proteins and vaccine products.

The present application relates to a kind of drug delivery system based on nucleic acid chemical modification and its preparation method and application.The drug delivery system includes nucleic acid aptamer coupling drug and antisense nucleic acid coupling drug, and the nucleic acid aptamer coupling drug and antisense nucleic acid coupling drug are co-assembled to form nanoparticle;The nucleic acid aptamer coupling drug includes nucleic acid aptamer and the chemotherapeutic drug coupled therewith;The antisense nucleic acid coupling drug includes antisense nucleic acid and the photosensitizer coupled therewith.The present application can reduce the up-regulation of the expression level of anti-apoptotic gene caused by chemotherapy and photodynamic therapy in tumor site by assembling tumor-targeting nucleic acid aptamer, chemotherapeutic drug, photosensitizer and antisense nucleic acid into drug delivery system, realizes the combination of chemotherapy, photodynamic therapy and gene therapy, efficiently inhibits tumor growth, and preparation method is simple and easy to operate, with universality, large drug loading, high production efficiency, it is expected to be applied in the treatment of various diseases, and promote the popularization and use of related products.

The present invention provides a method for producing human immortalized megakaryocytes and / or human immortalized megakaryocyte progenitor cells and / or human megakaryocyte cell lines (102) from adult CD34 + HSPC (100) capable of maturation into functional megakaryocytes (103) producing functional platelets (104), said method comprising at least the steps of: producing functional platelets (104) by transduction, in particular lentiviral (LV) transduction; an expression cassette comprising at least one gene is introduced into the CD34 + HSPC (100) to effectuate forced expression of the at least one gene wherein the transduced CD34 + HSPC (100) is configured to express at least one gene from the HOXL subclass of the ANTP homologous cassette family and / or the TALE homologous cassette family upon activator-induced expression, and constitutively expressing at least one anti-apoptotic gene from the BCL2 family wherein the transduced CD34 + HSPC (100) is immortalized by expression of the at least one gene from the ANTP homologous cassette family and / or the HOXL subclass of the TALE homologous cassette family, preferably wherein the at least one gene from the HOXL subclass comprises a combination of one of the HOXB8, HOXA7, HOXA10 or HOXB4 genes and at least one of the MEIS1, MEIS2, MEIS3, PBX1, PBX2, PBX3 and / or PBX4 genes, and the at least one anti-apoptosis gene from the BCL2 family comprises BCL2L1 or BCL2L2. The invention further provides a method of producing functional platelets by using the immortalized megakaryocytes and / or immortalized megakaryocyte progenitor cells and / or immortalized megakaryocyte cell lines obtained by the above method. Further, the present invention provides a pharmaceutical composition comprising functional platelets obtained by the above method and its therapeutic use.

This invention provides a brain polypeptide raw material with brain-boosting and intelligence-enhancing properties and its preparation method. The preparation method includes the following steps: S1, pretreatment; S2, first hydrolysis: adjusting the pH of the pre-hydrolysate to 6.5-7.5, adding neutral protease at 3.5-4.5% of bovine brain protein mass, hydrolyzing at 50-60℃ for 1-3 hours, and obtaining the first hydrolysate after enzyme inactivation; S3, second hydrolysis: adjusting the pH of the first hydrolysate to 6.0-7.0, adding papain at 1.0-3.0% of bovine brain protein mass, hydrolyzing at 45-65℃ for 1.5-2.5 hours, and obtaining the second hydrolysate after enzyme inactivation; S4, post-treatment, to obtain the brain polypeptide raw material powder. This brain polypeptide raw material can significantly scavenge DPPH and ABTS free radicals, inhibit acetylcholinesterase activity, repair PC12 nerve cells, inhibit the expression of the pro-apoptotic gene Bax and promote the expression of the anti-apoptotic gene Bcl-2, thus exerting a brain-boosting and intelligence-enhancing effect and protecting brain nerves.