Flavonoid glycosides, active extract of rose gold, preparation method and application
A technology of flavonoid glycosides and Phnom Penh rose, which is applied in the fields of antibacterial and antioxidation, and can solve problems such as safety allergies and hidden dangers of antibacterial agents
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Embodiment 1
[0053] The present embodiment prepares a kind of rose gold active extract, and the concrete steps are as follows:
[0054] Choose the dried rose of Phnom Penh, pulverize it, and extract it three times with an ethanol-water solution whose volume fraction is 95%. After filtration, the filtrate is concentrated to recover the solvent to obtain the first extract (that is, the 95% ethanol extract) The remaining plant residues (ie, filter residues) were extracted three times with a volume fraction of 65% ethanol-water solution, and after filtration, the filtrate was concentrated and recovered to obtain a second extract (ie, 65% ethanol extract).
[0055] After the first extract is suspended in water, it is extracted three times with petroleum ether to remove small polar components; the extraction residue is combined with the second extract, freeze-dried, and finally an ethanol extract is obtained.
[0056] The ethanol extract was dissolved in water, separated by macroporous resin (fi...
Embodiment 2
[0058] The present embodiment mainly separates the flavonoid glycosides from the rose gold; specifically includes the following steps:
[0059] 1) Preparation of Rose Phnom Penh active extract: For details of this step, refer to Example 1, which will not be repeated here.
[0060] 2) Separation of flavonoid glycosides from the active extracts of Rose Phnom Penh: take 15.68 g of Rose Phnom Penh active extracts, and then carry out silica gel column chromatography separation on the active extracts of Rose Phnom Penh; wherein, gradient washing is performed with a chloroform-methanol solvent system. The volume ratio of chloroform and methanol: 1:1-9:1; wherein, during gradient elution: the initial volume ratio of chloroform and methanol is 9:1, and the final volume ratio is 1:1; during gradient elution , the concentration of chloroform decreases); after elution, the elution fractions with a volume ratio of chloroform and methanol of 2:1-5:1 are combined to obtain the first fraction...
Embodiment 3
[0074] In this example, the antioxidative activity determination experiment is mainly carried out on the flavonoid glycosides compound prepared in Example 2 and the active extract of Rose Phnom Penh prepared in Example 1, and the determination method is as follows:
[0075] Antioxidant activity was measured using the DPPH free radical scavenging activity test. On a 96-well plate, the sample to be tested at the set concentration was mixed with DPPH (final concentration of 100 mM), and three replicate wells were set. At the same time, a blank control without drug and a positive control of Trolox were set, and placed at 30 °C for 1 h. After that, the OD value was measured by a microplate reader, and the detection wavelength was 515 nm.
[0076] Among them, the calculation formula of antioxidant rate is: antioxidant rate (%)=(1-experimental hole OD 515nm / blank hole OD 515 nm ) × 100%.
[0077] Among them, the measurement results of antioxidant activity are shown in Table 2. ...
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Abstract
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