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Macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active ingredients and preparation method of macroporous bone tissue engineering scaffold

A technology of tissue engineering scaffolds and active ingredients, which can be used in pharmaceutical formulations, filament/thread forming, textiles and papermaking, etc. It can solve the problems of lack of large pores for cell penetration and tissue regeneration, large energy consumption, and cumbersome processes, and achieve improvement. Insufficient biological activity in the early stage, increased pore size and surface roughness, and simple preparation method

Active Publication Date: 2020-08-14
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process of this technology is cumbersome, consumes a lot of energy, and the prepared phosphorus electrospun fibers are brittle, and the mechanical properties do not meet the requirements. At the same time, there is also a potential risk of "blood calcium"
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Method used

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  • Macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active ingredients and preparation method of macroporous bone tissue engineering scaffold
  • Macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active ingredients and preparation method of macroporous bone tissue engineering scaffold
  • Macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active ingredients and preparation method of macroporous bone tissue engineering scaffold

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Embodiment 1

[0030] This embodiment provides a macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active components, which is characterized in that it consists of a composite structure of micron-scale beads and nano-scale fibers prepared in one step by electrospinning, and the nano-scale fibers The inorganic active substances are released preferentially, and the inorganic active substances in the beads are subsequently released after the organic matrix of the beads is gradually degraded.

[0031] The preparation method of the macroporous bone tissue engineering scaffold capable of sustainably releasing the inorganic active ingredient:

[0032]Step 1: Dissolve 0.8g of PCL (number-average molecular weight: 80,000) in 8mL of dichloromethane (purity≥99.5%), and magnetically stir for 6h to obtain a clear and transparent polymer solution.

[0033] Step 2: Add 0.08 g of tricalcium phosphate into 2 mL of dichloromethane (purity ≥ 99.5%), and ultrasonically disp...

Embodiment 2

[0038] This embodiment provides a macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active components, which is characterized in that it consists of a composite structure of micron-scale beads and nano-scale fibers prepared in one step by electrospinning, and the nano-scale fibers The inorganic active substances are released preferentially, and the inorganic active substances in the beads are subsequently released after the organic matrix of the beads is gradually degraded.

[0039] The preparation method of the macroporous bone tissue engineering scaffold capable of sustainably releasing the inorganic active ingredient:

[0040] Step 1: Dissolve 0.5g chitosan (number average molecular weight 50,000-300,000, deacetylation degree: 90%) in 5mL 98wt% acetic acid solution, and magnetically stir for 12h to obtain a clear and transparent polymer solution.

[0041] Step 2: Add 0.04 g of silicon dioxide into 5 mL of 98 wt% acetic acid solution, an...

Embodiment 3

[0046] This embodiment provides a macroporous bone tissue engineering scaffold capable of sustainably releasing inorganic active components, which is characterized in that it consists of a composite structure of micron-scale beads and nano-scale fibers prepared in one step by electrospinning, and the nano-scale fibers The inorganic active substances are released preferentially, and the inorganic active substances in the beads are subsequently released after the organic matrix of the beads is gradually degraded.

[0047] The preparation method of the macroporous bone tissue engineering scaffold capable of sustainably releasing the inorganic active ingredient:

[0048] Step 1: Dissolve 0.6g PLLA (number-average molecular weight: 20,000) in 8mL chloroform, and stir magnetically for 12 hours to obtain a clear and transparent polymer solution.

[0049] Step 2: Add 0.1 g of hydroxyapatite into 2 mL of chloroform, and disperse by ultrasonic wave for 6 hours, so that the tricalcium ph...

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Abstract

The invention discloses a macroporous bone tissue engineering scaffold capable of continuously releasing inorganic active ingredients and a preparation method of the macroporous bone tissue engineering scaffold. The macroporous bone tissue engineering scaffold is composed of a composite structure of micron-sized beads and nano-sized fibers, wherein the composite structure is prepared in one step through electrostatic spinning, inorganic active substances in the nano-sized fibers are released preferentially, and after organic matrixes of the beads are degraded step by step, the inorganic activesubstances in the beads are released subsequently. The preparation method comprises the following steps: dropwise adding a dispersion liquid into a polymer solution to prepare an electrostatic spinning solution containing inorganic active ingredients; carrying out electrostatic spinning on the electrostatic spinning solution; carrying out vacuum drying on an obtained electrostatic spinning fibrous membrane; and then carrying out ethylene oxide treatment to finally obtain the macroporous bone tissue engineering scaffold which is rich in inorganic active ingredients and has a composite structure of micron-sized beads and nano-sized fibers. Due to existence of the beads, the pore diameter and the surface roughness of the scaffold can be increased, and infiltration growth and osteogenic differentiation of cells are facilitated. The preparation method disclosed by the invention is simple and short in process and is suitable for mass production.

Description

technical field [0001] The invention relates to a macroporous bone tissue engineering support capable of sustainably releasing inorganic active ingredients and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] Treatment of critically sized bone tissue defects resulting from trauma, tumors, and congenital diseases remains a major clinical challenge. It is estimated that there are more than 30 million cases of bone repair that require clinical transplantation every year worldwide. Due to good osseointegration, osteoconductivity, and osteoinductivity, autologous / allograft transplantation remains the gold standard for the treatment of such diseases. However, the limited number of grafts, damage to the donor site, and potential cross-infection limit the clinical use of autologous / allogeneic transplantation. With the development of bone tissue engineering (BTE), scaffold-based tissue engineering approaches provide an opti...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/44A61L27/56A61L27/58D01D5/00
CPCA61L27/446A61L27/46A61L27/56A61L27/58A61L2300/622A61L2400/12D01D5/0038
Inventor 刘来俊王富军张天添李超婧王璐张宇周俊泽
Owner DONGHUA UNIV