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Preparation method of injectable hydrogel, and application of injectable hydrogel to postoperative tumor treatment

A technology for injecting water and hydrogel, applied in the field of biomedicine, which can solve the problems of long hydrogel formation time, large enzyme dosage, and high enzymatic temperature, so as to prevent the formation time from being too long, high preparation efficiency, and good injection performance Effect

Active Publication Date: 2020-08-25
南京凯玛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the oligopeptide self-assembled hydrogels catalyzed by enzymes reported in the prior art often have problems such as long hydrogel formation time, large enzyme dosage, and high enzymatic temperature, which limit the use of hydrogels for postoperative cavity delivery. Application of Drug Carrier Construction

Method used

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  • Preparation method of injectable hydrogel, and application of injectable hydrogel to postoperative tumor treatment
  • Preparation method of injectable hydrogel, and application of injectable hydrogel to postoperative tumor treatment
  • Preparation method of injectable hydrogel, and application of injectable hydrogel to postoperative tumor treatment

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preparation example Construction

[0050] As mentioned above, the oligopeptide self-assembled hydrogels catalyzed by enzymes in the prior art often have problems such as long hydrogel formation time, large enzyme dosage, and high enzymatic temperature, which limit the use of hydrogels in the preparation of hydrogels. Application of postoperative cavity drug delivery system construction. Therefore, the present invention provides an enzyme-responsive injectable hydrogel and its preparation method and application. The enzyme-responsive injectable hydrogel has a short and suitable formation time, a suitable enzymatic temperature, and a small amount of enzyme. biocompatible drug carrier.

[0051] The enzyme-catalyzed cross-linked hydrogel constructed in a particularly excellent embodiment of the present invention has a 3D network structure, maintains high elasticity and is safe and harmless.

[0052] According to the present invention, the injectable hydrogel is injectable before incubation, can be incubated for a ...

Embodiment 1

[0061] Example 1: Enzyme-responsive injectable hydrogel preparation method

[0062] (1) Take a clean and dry 5mL transparent glass bottle, accurately weigh a certain amount of Fmoc-F and FF-Dopa powder, add 2mL pH=8.5 phosphate buffer solution, so that the final concentration of Fmoc-F is 20mM, and the final concentration of FF-Dopa Concentration is 40mM, miscible and add magnetic stir bar.

[0063]

[0064]

[0065] (2) Take a clean and dry 25mL small beaker, accurately weigh a certain amount of sodium hydroxide powder, add double distilled water and stir it with a glass rod to dissolve it, the final concentration of NaOH solution is 1M. Place the above-mentioned transparent glass bottle on a magnetic stirrer, stir at a constant speed of 50rpm / min, slowly add NaOH solution to the mixed solution in the glass bottle with a dropper while stirring, and constantly use a pH meter to detect the pH of the mixed solution during the dropping process , so that the overall solutio...

Embodiment 2

[0070] Example 2: Enzyme-responsive injectable hydrogel preparation method

[0071] (1) Take a clean and dry 5mL transparent glass bottle, accurately weigh a certain amount of Fmoc-F and FF-Dopa powder, add 2mL pH=8.5 bicarbonate buffer solution, so that the final concentration of Fmoc-F is 10 mM, FF-Dopa -Dopa final concentration is 30mM, mix and add magnetic stir bar.

[0072](2) Take a clean and dry 25mL small beaker, accurately weigh a certain amount of sodium hydroxide powder, add double distilled water and stir it with a glass rod to dissolve it, the final concentration of NaOH solution is 1M. Place the above-mentioned transparent glass bottle on a magnetic stirrer, stir at a constant speed of 60rpm / min, slowly add NaOH solution to the mixed solution in the glass bottle with a dropper while stirring, and constantly use a pH meter to detect the pH of the mixed solution during the dropping process , so that the overall solution pH does not exceed 8.5.

[0073] (3) Take a...

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Abstract

The invention belongs to the technical field of biomedicine, and relates to a preparation method of injectable hydrogel, and an application of the injectable hydrogel to of postoperative tumor treatment. Thermolysin is used for catalyzing N-( 9-N-( 9-fluorenylmethyloxycarbonyl)-L-phenylalanine(Fmoc-F) and L- phenylalanine-L- phenylalanine-L-L-dopa(FF-Dopa) to form an Fmoc-F-FF-Dopa unit. The unitfurther utilizes hydrogen bonds, hydrophobic interaction, phi-phi accumulation and other non-covalent bond force, so that self assembly under the temperature condition being similar to the temperatureof a human body within a short time is performed to form the hydrogel. The enzyme catalysis cross-linking hydrogel has syringeability, and can be implanted into a cavity formed by a tumor operation as a medicine storing house, so that the carried medicines are slowly released in the local part of the cavity formed after the operation at a stable and controllable rate and with an appropriate concentration, drug effects are sufficiently exerted, remaining tumor cells are killed, and toxic and side effect caused by systemic administration can be avoided.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method of an injectable hydrogel and its application in postoperative tumor treatment. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Malignant tumors have become one of the important social problems that seriously threaten people's health, with rapid disease progression, high mortality and low five-year survival rate. At present, for breast cancer, glioma, melanoma and other malignant tumors, surgical resection is often used clinically. Partial or most of the primary or metastatic lesions are removed to reduce the tumor burden and relieve the cl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/34A61P35/00
CPCA61K9/0019A61K9/06A61K47/34A61P35/00
Inventor 姜新义张晶陈晨
Owner 南京凯玛生物科技有限公司
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