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Venom-based peptide and application thereof

A venom and amino acid technology, applied in the direction of peptides, embryonic cells, germ cells, etc., to achieve the effect of promoting self-renewal

Active Publication Date: 2020-08-25
UNIVERSITY OF MACAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite their self-renewal potential, stem cells require extremely high regenerative conditions to maintain their pluripotency

Method used

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  • Venom-based peptide and application thereof
  • Venom-based peptide and application thereof
  • Venom-based peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] This example provides venom-based peptide P13 and mutant M6 of P13.

[0093] The amino acid sequence of P13 is shown in SEQ ID No.1, and the amino acid sequence of M6 is shown in SEQ ID No.2.

[0094] P13 is mutated to M6:

[0095] P13 was mutated to synthesize its derivative P13-M5 (sequence shown in SEQ ID No.3). It was reported in the literature that the binding affinity of the basic amino acid arginine to heparin is 2.5 times that of lysine, so further mutations resulted in P13-M6.

Embodiment 2

[0097] This embodiment provides a medium for culturing human embryonic stem cells, specifically as follows.

[0098] (1) E6 and P13;

[0099] (2) E6 and M6;

[0100] (3) E8 and P13;

[0101] (4) E8 and M6.

[0102] It should be noted that in (1) to (4), the concentrations of P13 and M6 were 100 μM. Specifically, the E6 medium includes: DMEM / F12, insulin, L-ascorbic acid, selenium, NaHCO 3 and transferrin;

[0103] E8 medium includes: DMEM / F12, FGF2, TGFβ1, insulin, L-ascorbic acid, selenium, NaHCO 3 and transferrin.

[0104] Human ES cell culture

[0105] Taking E8 medium as an example, human ES cells (hESC, H1) were maintained in E8 medium using matrigel-coated tissue culture plates. E8 medium has eight components: DMEM / F12, L-ascorbic acid-2-magnesium phosphate (64mg / L), sodium selenium (14μg / L), insulin (19.4mg / L), NaHCO3 (543mg / L), Transferrin (10.7 mg / L) and FGF2 (100 μg / L) and TGFβ1 (2 μg / L) growth factors.

[0106] Once the cells reached 80-85% confluency, cel...

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Abstract

The invention discloses a venom-based peptide and application thereof, and relates to the technical field of biomolecules, the amino acid sequence of the peptide is shown as formula 1: X1-X2-X3-X4-X5-X6-X7-X8-X9-X10-X11-X12-X13-X14-X15-X16-X17-X18; wherein X1, X5, X6, X9 and X11 are all selected from any one of A, V, L, I, M, F, W and P; X2, X3, X10, X15, X17 and X18 are selected from any one of G, C, S, T, Y, N and Q; X7 is selected from D or E; X4, X8 and X16 are all selected from any one of K, R and H; X12, X13 and X14 are all selected from any one of K, R, H, D and E. The peptide has the function of promoting self-renewal of human embryonic stem cells.

Description

technical field [0001] The invention relates to the technical field of biomolecules, in particular to a venom-based peptide and its application. Background technique [0002] The advantage of venom peptides from animals such as scorpions, spiders and snakes is that they have a stable structure and have undergone adaptive evolution over millions of years to be highly specific for target molecules. [0003] Human embryonic stem cell (hESC) therapy has been proposed for regenerative medicine and tissue replacement following injury or disease, such as Parkinson's disease, spinal cord injury and diabetes. Despite their self-renewal potential, stem cells require extremely high regenerative conditions to maintain their pluripotency. [0004] In view of this, the present invention is proposed. Contents of the invention [0005] The purpose of the present invention is to provide a venom-based peptide and its application. [0006] The present invention is achieved like this: [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C12N5/0735
CPCC07K7/08C12N5/0606C12N2501/998C12N2500/80C12N2500/05C12N2500/25C12N2501/10C12N2501/602C12N2501/603C12N2501/605
Inventor 郭珩辉马瑞陈国凯
Owner UNIVERSITY OF MACAU